<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Pereira-Leal, José B</style></author><author><style face="normal" font="default" size="100%">Abreu, Isabel a</style></author><author><style face="normal" font="default" size="100%">Alabaça, Cláudia S</style></author><author><style face="normal" font="default" size="100%">Almeida, Maria Helena</style></author><author><style face="normal" font="default" size="100%">Almeida, Paulo</style></author><author><style face="normal" font="default" size="100%">Almeida, Tânia</style></author><author><style face="normal" font="default" size="100%">Amorim, Maria Isabel</style></author><author><style face="normal" font="default" size="100%">Araújo, Susana</style></author><author><style face="normal" font="default" size="100%">Azevedo, Herlânder</style></author><author><style face="normal" font="default" size="100%">Badia, Aleix</style></author><author><style face="normal" font="default" size="100%">Batista, Dora</style></author><author><style face="normal" font="default" size="100%">Bohn, Andreas</style></author><author><style face="normal" font="default" size="100%">Capote, Tiago</style></author><author><style face="normal" font="default" size="100%">Carrasquinho, Isabel</style></author><author><style face="normal" font="default" size="100%">Chaves, Inês</style></author><author><style face="normal" font="default" size="100%">Coelho, Ana Cristina</style></author><author><style face="normal" font="default" size="100%">Costa, Maria Manuela Ribeiro</style></author><author><style face="normal" font="default" size="100%">Costa, Rita</style></author><author><style face="normal" font="default" size="100%">Cravador, Alfredo</style></author><author><style face="normal" font="default" size="100%">Egas, Conceição</style></author><author><style face="normal" font="default" size="100%">Faro, Carlos</style></author><author><style face="normal" font="default" size="100%">Fortes, Ana M</style></author><author><style face="normal" font="default" size="100%">Fortunato, Ana S</style></author><author><style face="normal" font="default" size="100%">Gaspar, Maria João</style></author><author><style face="normal" font="default" size="100%">Gonçalves, Sónia</style></author><author><style face="normal" font="default" size="100%">Graça, José</style></author><author><style face="normal" font="default" size="100%">Horta, Marília</style></author><author><style face="normal" font="default" size="100%">Inácio, Vera</style></author><author><style face="normal" font="default" size="100%">Leitão, José M</style></author><author><style face="normal" font="default" size="100%">Lino-Neto, Teresa</style></author><author><style face="normal" font="default" size="100%">Marum, Liliana</style></author><author><style face="normal" font="default" size="100%">Matos, José</style></author><author><style face="normal" font="default" size="100%">Mendonça, Diogo</style></author><author><style face="normal" font="default" size="100%">Miguel, Andreia</style></author><author><style face="normal" font="default" size="100%">Miguel, Célia M</style></author><author><style face="normal" font="default" size="100%">Morais-Cecílio, Leonor</style></author><author><style face="normal" font="default" size="100%">Neves, Isabel</style></author><author><style face="normal" font="default" size="100%">Nóbrega, Filomena</style></author><author><style face="normal" font="default" size="100%">Oliveira, Maria Margarida</style></author><author><style face="normal" font="default" size="100%">Oliveira, Rute</style></author><author><style face="normal" font="default" size="100%">Pais, Maria Salomé</style></author><author><style face="normal" font="default" size="100%">Paiva, Jorge a</style></author><author><style face="normal" font="default" size="100%">Paulo, Octávio S</style></author><author><style face="normal" font="default" size="100%">Pinheiro, Miguel</style></author><author><style face="normal" font="default" size="100%">Raimundo, João Ap</style></author><author><style face="normal" font="default" size="100%">Ramalho, José C</style></author><author><style face="normal" font="default" size="100%">Ribeiro, Ana I</style></author><author><style face="normal" font="default" size="100%">Ribeiro, Teresa</style></author><author><style face="normal" font="default" size="100%">Rocheta, Margarida</style></author><author><style face="normal" font="default" size="100%">Rodrigues, Ana Isabel</style></author><author><style face="normal" font="default" size="100%">Rodrigues, José C</style></author><author><style face="normal" font="default" size="100%">Saibo, Nelson Jm</style></author><author><style face="normal" font="default" size="100%">Santo, Tatiana E</style></author><author><style face="normal" font="default" size="100%">Santos, Ana Margarida</style></author><author><style face="normal" font="default" size="100%">Sá-Pereira, Paula</style></author><author><style face="normal" font="default" size="100%">Sebastiana, Mónica</style></author><author><style face="normal" font="default" size="100%">Simões, Fernanda</style></author><author><style face="normal" font="default" size="100%">Sobral, Rómulo S</style></author><author><style face="normal" font="default" size="100%">Tavares, Rui</style></author><author><style face="normal" font="default" size="100%">Teixeira, Rita</style></author><author><style face="normal" font="default" size="100%">Varela, Carolina</style></author><author><style face="normal" font="default" size="100%">Veloso, Maria Manuela</style></author><author><style face="normal" font="default" size="100%">Ricardo, Cândido Pp</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A comprehensive assessment of the transcriptome of cork oak (Quercus suber) through EST sequencing.</style></title><secondary-title><style face="normal" font="default" size="100%">BMC genomics</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">EST sequencing</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">transcriptome</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year></dates><volume><style face="normal" font="default" size="100%">15</style></volume><pages><style face="normal" font="default" size="100%">371</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">BACKGROUND: Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic information. Cork oak forests are of great ecological importance and represent a major economic and social resource in Southern Europe and Northern Africa. However, global warming is threatening the cork oak forests by imposing thermal, hydric and many types of novel biotic stresses. Despite the economic and social value of the Q. suber species, few genomic resources have been developed, useful for biotechnological applications and improved forest management. RESULTS: We generated in excess of 7 million sequence reads, by pyrosequencing 21 normalized cDNA libraries derived from multiple Q. suber tissues and organs, developmental stages and physiological conditions. We deployed a stringent sequence processing and assembly pipeline that resulted in the identification of ~159,000 unigenes. These were annotated according to their similarity to known plant genes, to known Interpro domains, GO classes and E.C. numbers. The phylogenetic extent of this ESTs set was investigated, and we found that cork oak revealed a significant new gene space that is not covered by other model species or EST sequencing projects. The raw data, as well as the full annotated assembly, are now available to the community in a dedicated web portal at http://www.corkoakdb.org. CONCLUSIONS: This genomic resource represents the first trancriptome study in a cork producing species. It can be explored to develop new tools and approaches to understand stress responses and developmental processes in forest trees, as well as the molecular cascades underlying cork differentiation and disease response.</style></abstract><accession-num><style face="normal" font="default" size="100%">24885229</style></accession-num><notes><style face="normal" font="default" size="100%">APS</style></notes><research-notes><style face="normal" font="default" size="100%">APS</style></research-notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Graça, José</style></author><author><style face="normal" font="default" size="100%">Pereira, Helena</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">The periderm development in Quercus suber</style></title><secondary-title><style face="normal" font="default" size="100%">IAWA Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cork</style></keyword><keyword><style  face="normal" font="default" size="100%">epidermis</style></keyword><keyword><style  face="normal" font="default" size="100%">lenticels</style></keyword><keyword><style  face="normal" font="default" size="100%">phellem</style></keyword><keyword><style  face="normal" font="default" size="100%">phellogen</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2004</style></year></dates><volume><style face="normal" font="default" size="100%">25</style></volume><pages><style face="normal" font="default" size="100%">325-335</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">In the cork oak (Quercus suber L.), the phellogen differentiates during the first year of growth in the cell layer immediately under the epidermis and divides to form 3–6 suberized phellem cells. Division of the phellogen only occurs after suberization of the previous divided cell. During the first four years of growth, the phellem cells have tannin-filled lumens and it is only in the 5th to 7th years that they acquire the characteristics of ʻadultʼ cork cells with empty lumens and thin suberized walls. The len- ticels are formed by the lenticular phellogen, which differentiates under the stomata and has a high meristematic activity. In this region, the cells are unsuberized, with a loose arrangement and intercellular voids, consti- tuting the filling or complementary tissue. After three years, the lenticels appear as small protuberances that soon become conspicuous. Inclusions of sclerenchymatous nodules and isolated sclereids occur occasionally mostly in the vicinity of, or in, the lenticels.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Graça, José</style></author><author><style face="normal" font="default" size="100%">Pereira, Helena</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cork Suberin: A Glyceryl Based Polyester</style></title><secondary-title><style face="normal" font="default" size="100%">Holzforschung - International Journal of the Biology, Chemistry, Physics and Technology of Wood</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cork</style></keyword><keyword><style  face="normal" font="default" size="100%">Glycerol</style></keyword><keyword><style  face="normal" font="default" size="100%">Monoacylglycero</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">suberin</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1997</style></year></dates><volume><style face="normal" font="default" size="100%">51</style></volume><pages><style face="normal" font="default" size="100%">225-234</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Total depolymerization of cork from the cork oak (Quercus suber L.) by using a sodium methoxide catalysed methanolysis solubilized 53.2 % of the material, including 5.2 % of glycerol, 48,0 % of suberinic fatty acids and alcohols and minor amounts of ferulic acid. A very mild depolymerization using calcium oxide treated methanol, which solubilized only 2.0% of the cork material was studied by GC-MS. In the solubilized material, the total amount of aliphatic acids was 43.8 % (including alkanoic acids 4.0%. ω-hydroxyacids 13.2% and α,ω-diacids 26.6%), of 1-alkanols 2.1 % and of monoacylglycenols 32.1%. It was possible to identify 1-monoacylglycerols and 2-monoacylglycerols of alkanoic acids (1,2%), ω-hydroxyacids (3.7 %) and α,ω-diacids (22.8 %). It is proposed that suberin is a glyceryl based polymer and that its insoluble character is given, at least in part, by the cross-linking of dicarboxylic fatty acids with glycerol. The term suberin should be used for this aliphatic polyester component of the cell wall.</style></abstract></record></records></xml>