<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Blasco, M.</style></author><author><style face="normal" font="default" size="100%">Barra, A.</style></author><author><style face="normal" font="default" size="100%">Brisa, C.</style></author><author><style face="normal" font="default" size="100%">Corredoira, E.</style></author><author><style face="normal" font="default" size="100%">Segura, J.</style></author><author><style face="normal" font="default" size="100%">Toribio, M.</style></author><author><style face="normal" font="default" size="100%">Arrillaga, I.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Somatic embryogenesis in holm oak male catkins</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Growth Regulation</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">adult trees</style></keyword><keyword><style  face="normal" font="default" size="100%">developmental stage</style></keyword><keyword><style  face="normal" font="default" size="100%">flow cytometry</style></keyword><keyword><style  face="normal" font="default" size="100%">forest biotechnology</style></keyword><keyword><style  face="normal" font="default" size="100%">histology</style></keyword><keyword><style  face="normal" font="default" size="100%">micropropagation</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">secondary embryogenesis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2013///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://link.springer.com/10.1007/s10725-013-9826-3</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">71</style></volume><pages><style face="normal" font="default" size="100%">261 - 270</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Somatic embryogenesis (SE) of tree species is the most promising method for the implementation of multivarietal forestry and for biotechnological approaches. To date, however, the application of this technology to mature trees is restricted to a few species. This is the first report on the induction of SE from male catkins of 100-year-old holm oaks (Quercus ilex L.). Embryogenic competence was mainly dependent on genotype and restricted to the most advanced catkin developmental stage with distinguishable closed flowers along the axis. Fol- lowing a three-stage treatment procedure, embryogenic response (frequencies up 3.3 %) was obtained in three [Remedio, Villar del Arzobispo (VA) and Hunde (HU)] out of the five genotypes evaluated. In the culture conditions tested, the preferred protocol to induce SE in holm oak catkins should include: induction on MS medium with 6-benzyladenine and naphthaleneacetic acid, subculture onto medium with a reduced concentration of both plant growth regulators and a final transference to medium without growth regulators. Under these conditions cotyledonary-stage cotyledonary-stage somatic embryos developed from brown calli with or without nodular structures. Secondary SE, favored by the addition of sorbitol to the manifestation medium, allowed the establishment of 14 embryogenic lines belonging to VA and HU genotypes. Histological observations of the proliferating cultures revealed the presence of globular, torpedo and cotyledonary somatic embryos. Somatic embryos were diploid as verified by flow cytometry analysis, suggesting that they originated from the perianthic tissue of the male flower</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>47</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Fernández Guijarro, B.</style></author><author><style face="normal" font="default" size="100%">Celestino, C.</style></author><author><style face="normal" font="default" size="100%">Toribio, M.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">OBTENCION DE PLANTAS A PARTIR DE EMBRIONES SOMATICOS DE QUERCUS SUBER L</style></title><secondary-title><style face="normal" font="default" size="100%">Congreso Forestal Español, Lourizán - Pontevedra. 1993</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">adventive embryogenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">culture media</style></keyword><keyword><style  face="normal" font="default" size="100%">plant regeneration</style></keyword><keyword><style  face="normal" font="default" size="100%">secondary embryogenesis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1993</style></year><pub-dates><date><style  face="normal" font="default" size="100%">1993///</style></date></pub-dates></dates><pages><style face="normal" font="default" size="100%">337 - 341</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">This paper gives a procedure to regenerate plants of cork oak through somatic embryogenesis. The somatic embryos were obtained in leaves from young seedlings and cotyledonary fragments from mature acorns of cork oak. The explants were cultured in three stage process in wich BA and NAA concentration were decreasing. Different methods were used to germinate the embryos. Dessication and culture in low salt concentration medium were chosen to give the best results</style></abstract><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;periodical: Congreso Forestal Español, Lourizán - Pontevedra. 1993</style></notes></record></records></xml>