<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors></contributors><titles><title><style face="normal" font="default" size="100%">Growth data from a field trial of Quercus suber plants regenerated from selected trees and from their half-sib progenies by somatic embryogenesis</style></title><secondary-title><style face="normal" font="default" size="100%">Acta Horticulturae</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2009</style></year></dates><volume><style face="normal" font="default" size="100%">812</style></volume><pages><style face="normal" font="default" size="100%">493-498</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The development of reliable clonal propagation technologies is a requisite for performing Multi-Varietal Forestry (MVF). Somatic embryogenesis is considered the tissue culture based method more suitable for operational breeding of forest trees. Vegetative propagation is very difficult when tissues are taken from mature donors, making clonal propagation of selected trees almost impossible. We have been able to induce somatic embryogenesis in leaves taken from mature oak trees, including cork oak (Quercus suber). This important species of the Mediterranean ecosystem produces cork regularly, conferring to this species a significant economic value. In a previous paper we reported the establishment of a field trial to compare the growth of plants of somatic origin vs zygotic origin, and somatic plants from mature trees vs somatic plants from juvenile seedlings. For that purpose somatic seedlings were regenerated from five selected cork oak trees and from young plants of their half-sib progenies by somatic embryogenesis. They were planted in the field together with acorn-derived plants of the same families. After the first growth period, seedlings of zygotic origin doubled the height of somatic seedlings, showing somatic plants of adult and juvenile origin similar growth. Here we provide data on height and diameter increases after two additional growth periods. In the second one, growth parameters of zygotic seedlings were also significantly higher than those of somatic ones, but there were not significant differences in height increase between seedlings and somatic plants of mature origin. In the third growth period, height and diameter increases of somatic seedlings cloned from the selected trees did not differ from those of zygotic seedlings, which were still higher than data from plants obtained from somatic embryos from the sexual progeny. Therefore, somatic seedlings from mature origin seem not to be influenced by a possible ageing effect, and plants from somatic embryos tend to minimize the initial advantage of plants from acorns.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Celestino, Cristina</style></author><author><style face="normal" font="default" size="100%">Fernandez-Guijarro, B.</style></author><author><style face="normal" font="default" size="100%">Hernández, Inmaculada</style></author><author><style face="normal" font="default" size="100%">López Vela, Dolores</style></author><author><style face="normal" font="default" size="100%">Carneros, Elena</style></author><author><style face="normal" font="default" size="100%">Jiménez García, Jesús</style></author><author><style face="normal" font="default" size="100%">Cardo, L.</style></author><author><style face="normal" font="default" size="100%">Alegre, Jesús</style></author><author><style face="normal" font="default" size="100%">Toribio, Mariano</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Growth data from a field trial of Quercus suber plants regenerated from selected trees and from their half-sib progenies by somatic embryogenesis</style></title><secondary-title><style face="normal" font="default" size="100%">Acta Horticulturae</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">field establishment</style></keyword><keyword><style  face="normal" font="default" size="100%">forest improvement</style></keyword><keyword><style  face="normal" font="default" size="100%">rejuvenation</style></keyword><keyword><style  face="normal" font="default" size="100%">vegetative propagation</style></keyword><keyword><style  face="normal" font="default" size="100%">woody plant micropropagation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2009</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2009///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.actahort.org/books/812/812_71.htm</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">812</style></volume><pages><style face="normal" font="default" size="100%">493 - 498</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The development of reliable clonal propagation technologies is a requisite for performing Multi-Varietal Forestry (MVF). Somatic embryogenesis is considered the tissue culture based method more suitable for operational breeding of forest trees. Vegetative propagation is very difficult when tissues are taken from mature donors, making clonal propagation of selected trees almost impossible. We have been able to induce somatic embryogenesis in leaves taken from mature oak trees, including cork oak (Quercus suber). This important species of the Mediterranean ecosystem produces cork regularly, conferring to this species a significant economic value. In a previous paper we reported the establishment of a field trial to compare the growth of plants of somatic origin vs zygotic origin, and somatic plants from mature trees vs somatic plants from juvenile seedlings. For that purpose somatic seedlings were regenerated from five selected cork oak trees and from young plants of their half-sib progenies by somatic embryogenesis. They were planted in the field together with acorn-derived plants of the same families. After the first growth period, seedlings of zygotic origin doubled the height of somatic seedlings, showing somatic plants of adult and juvenile origin similar growth. Here we provide data on height and diameter increases after two additional growth periods. In the second one, growth parameters of zygotic seedlings were also significantly higher than those of somatic ones, but there were not significant differences in height increase between seedlings and somatic plants of mature origin. In the third growth period, height and diameter increases of somatic seedlings cloned from the selected trees did not differ from those of zygotic seedlings, which were still higher than data from plants obtained from somatic embryos from the sexual progeny. Therefore, somatic seedlings from mature origin seem not to be influenced by a possible ageing effect, and plants from somatic embryos tend to minimize the initial advantage of plants from acorns.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>7</ref-type><contributors></contributors><titles><title><style face="normal" font="default" size="100%">Cork Oak Trees (Quercus suber L.).</style></title><secondary-title><style face="normal" font="default" size="100%">Methods in molecular biology (Clifton, N.J.)</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2006</style></year></dates><publisher><style face="normal" font="default" size="100%">Humana Press</style></publisher><pub-location><style face="normal" font="default" size="100%">Totowa</style></pub-location><volume><style face="normal" font="default" size="100%">344</style></volume><pages><style face="normal" font="default" size="100%">113-123</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">A transformation system for selected mature Quercus suber L. trees using Agrobacterium tumefaciens has been established. Embryos obtained from recurrent proliferating embryogenic masses are inoculated with AGL1 strain harbouring the plasmid pBINUbiGUSint, which carries the nptII and uidA genes. Evidence of stable transgene integration is obtained by polymerase chain reaction for nptII and uidA genes, Southern blotting and expression of the uidA gene. The transgenic embryos are germinated and successfully transferred to soil.</style></abstract><accession-num><style face="normal" font="default" size="100%">17033056</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>5</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Alvarez, Rubén</style></author><author><style face="normal" font="default" size="100%">Toribio, Mariano</style></author><author><style face="normal" font="default" size="100%">Cortizo, Millán</style></author><author><style face="normal" font="default" size="100%">Ordás Fernández, Ricardo-Javier</style></author></authors><secondary-authors><author><style face="normal" font="default" size="100%">Wang, K.</style></author></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Cork Oak Trees (Quercus suber L.).</style></title><secondary-title><style face="normal" font="default" size="100%">Methods in molecular biology (Clifton, N.J.)</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">AGL1</style></keyword><keyword><style  face="normal" font="default" size="100%">Agrobacterium tumefaciens</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Fagaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">kanamycin resistance</style></keyword><keyword><style  face="normal" font="default" size="100%">pBINUbiGUSint</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">Somatic embryogenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Tree genetic transformation</style></keyword><keyword><style  face="normal" font="default" size="100%">β-glucuronidase</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2006</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2006///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/17033056</style></url></web-urls></urls><publisher><style face="normal" font="default" size="100%">Humana Press</style></publisher><pub-location><style face="normal" font="default" size="100%">Totowa</style></pub-location><volume><style face="normal" font="default" size="100%">344</style></volume><pages><style face="normal" font="default" size="100%">113 - 123</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">A transformation system for selected mature Quercus suber L. trees using Agrobacterium tumefaciens has been established. Embryos obtained from recurrent proliferating embryogenic masses are inoculated with AGL1 strain harbouring the plasmid pBINUbiGUSint, which carries the nptII and uidA genes. Evidence of stable transgene integration is obtained by polymerase chain reaction for nptII and uidA genes, Southern blotting and expression of the uidA gene. The transgenic embryos are germinated and successfully transferred to soil.</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;periodical: Methods in molecular biology (Clifton, N.J.)&lt;br/&gt;accession-num: 17033056&lt;br/&gt;electronic-resource-num: 10.1385/1-59745-131-2:113</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Dı́ez, Jesús</style></author><author><style face="normal" font="default" size="100%">Manjón, José Luis</style></author><author><style face="normal" font="default" size="100%">Kovács, Gabor M.</style></author><author><style face="normal" font="default" size="100%">Celestino, Cristina</style></author><author><style face="normal" font="default" size="100%">Toribio, Mariano</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Mycorrhization of vitroplants raised from somatic embryos of cork oak (Quercus suber L.)</style></title><secondary-title><style face="normal" font="default" size="100%">Applied Soil Ecology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">ectomycorrhizas</style></keyword><keyword><style  face="normal" font="default" size="100%">Mycorrhization</style></keyword><keyword><style  face="normal" font="default" size="100%">Somatic embryogenesis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2000</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2000///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://linkinghub.elsevier.com/retrieve/pii/S0929139300000871</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">15</style></volume><pages><style face="normal" font="default" size="100%">119 - 123</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The technique described herein allows in vitro ectomycorrhizal synthesis in Quercus suber vitroplants raised from somatic embryos with Pisolithus tinctorius and Scleroderma polyrhizum strains. Only strains of this species coming from fruit bodies collected in Quercus suber stands (strain QS241 and strain QS247) formed ectomycorrhizas, and hence these species seem to exhibit host adaptation. The in vitro mycorrhization facilitated the development of secondary roots and the ex vitro weaning of cork oak vitroplants.</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors></contributors><titles><title><style face="normal" font="default" size="100%">Mycorrhization of vitroplants raised from somatic embryos of cork oak (Quercus suber L.)</style></title><secondary-title><style face="normal" font="default" size="100%">Applied Soil Ecology</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2000</style></year></dates><volume><style face="normal" font="default" size="100%">15</style></volume><pages><style face="normal" font="default" size="100%">119-123</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The technique described herein allows in vitro ectomycorrhizal synthesis in Quercus suber vitroplants raised from somatic embryos with Pisolithus tinctorius and Scleroderma polyrhizum strains. Only strains of this species coming from fruit bodies collected in Quercus suber stands (strain QS241 and strain QS247) formed ectomycorrhizas, and hence these species seem to exhibit host adaptation. The in vitro mycorrhization facilitated the development of secondary roots and the ex vitro weaning of cork oak vitroplants.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Fernández-Guijarro, Bárbara</style></author><author><style face="normal" font="default" size="100%">Celestino, Cristina</style></author><author><style face="normal" font="default" size="100%">Toribio, Mariano</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Influence of external factors on secondary embryogenesis and germination in somatic embryos from leaves of Quercus suber</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Cell, Tissue and Organ Culture</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">culture media</style></keyword><keyword><style  face="normal" font="default" size="100%">plant regeneration</style></keyword><keyword><style  face="normal" font="default" size="100%">recurrent embryogenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">repetitive embryogenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Somatic embryogenesis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1995</style></year><pub-dates><date><style  face="normal" font="default" size="100%">1995///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.springerlink.com/index/10.1007/BF00051578</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">41</style></volume><pages><style face="normal" font="default" size="100%">99 - 106</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Somatic embryogenesis was obtained in cultures of leaves from young seedlings of Quercus suber L. A two- stage process, in which benzyladenine and naphthaleneacetic acid were added first at high and then at low concentrations, was required to initiate the process. Somatic embryos arose when the explants were subsequently placed on medium lacking plant growth regulators. The embryogenic lines remained productive, by means of secondary embryogenesis, on medium without growth regulators. However, this repetitive induction was influenced by the macronutrient composition of the culture medium. Both low total nitrogen content and high reduced nitrogen concentration decreased the percentage of somatic embryos that showed secondary embryogenesis. Our results suggest that alternate culture on medium that increases embryo proliferation and a low salt medium prohibiting embryo formation will partially synchronize embryo development. Chilling slightly reduced secondary embryogenesis but gave a modest increase in germination. Maturation under light followed by storage at 4 °C for at least 30 days gave the best results in switching embryos from an embryogenic pathway to a germinative one. Under these conditions 15% of embryos showed coordinated root and shoot growth and 35% formed either shoots or mostly roots. These percentages were higher than those of embryos matured in darkness. This result indicates that a specific treatment is required after maturation and before chilling to activate the switch from secondary embryo formation to germination.</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors></contributors><titles><title><style face="normal" font="default" size="100%">Influence of external factors on secondary embryogenesis and germination in somatic embryos from leaves of Quercus suber</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Cell, Tissue and Organ Culture</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">1995</style></year></dates><volume><style face="normal" font="default" size="100%">41</style></volume><pages><style face="normal" font="default" size="100%">99-106</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Somatic embryogenesis was obtained in cultures of leaves from young seedlings of Quercus suber L. A two- stage process, in which benzyladenine and naphthaleneacetic acid were added first at high and then at low concentrations, was required to initiate the process. Somatic embryos arose when the explants were subsequently placed on medium lacking plant growth regulators. The embryogenic lines remained productive, by means of secondary embryogenesis, on medium without growth regulators. However, this repetitive induction was influenced by the macronutrient composition of the culture medium. Both low total nitrogen content and high reduced nitrogen concentration decreased the percentage of somatic embryos that showed secondary embryogenesis. Our results suggest that alternate culture on medium that increases embryo proliferation and a low salt medium prohibiting embryo formation will partially synchronize embryo development. Chilling slightly reduced secondary embryogenesis but gave a modest increase in germination. Maturation under light followed by storage at 4 °C for at least 30 days gave the best results in switching embryos from an embryogenic pathway to a germinative one. Under these conditions 15% of embryos showed coordinated root and shoot growth and 35% formed either shoots or mostly roots. These percentages were higher than those of embryos matured in darkness. This result indicates that a specific treatment is required after maturation and before chilling to activate the switch from secondary embryo formation to germination.</style></abstract></record></records></xml>