<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Correia, Barbara</style></author><author><style face="normal" font="default" size="100%">Rodriguez, José Luis</style></author><author><style face="normal" font="default" size="100%">Valledor, Luis</style></author><author><style face="normal" font="default" size="100%">Almeida, Tânia</style></author><author><style face="normal" font="default" size="100%">Santos, Conceição</style></author><author><style face="normal" font="default" size="100%">Cañal, Maria Jesus</style></author><author><style face="normal" font="default" size="100%">Pinto, Glória</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Analysis of the expression of putative heat-stress related genes in relation to thermotolerance of cork oak</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Plant Physiology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">high temperature</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant performance</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">Recovery</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year></dates><volume><style face="normal" font="default" size="100%">171</style></volume><pages><style face="normal" font="default" size="100%">399-406</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Cork oak (Quercus suber L.) is a research priority in the Mediterranean area and because of cork oaks’ dis- tribution these stands are experiencing daily stress. Based on projections of intensifying climate change and considering the key role of exploring the recovery abilities, cork oak seedlings were subjected to a cumulative temperature increase from 25◦C to 55◦C and subsequent recovery. CO2 assimilation rate, chlorophyll fluorescence, anthocyanins, proline and lipid peroxidation were used to evaluate plant per- formance, while the relative abundance of seven genes encoding for proteins of cork oak with a putative role in thermal/stress regulation (POX1, POX2, HSP10.4, HSP17a.22, CHS, MTL and RBC) was analyzed by qPCR (quantitative Polymerase Chain Reaction). A temperature change to 35◦C showed abundance alterations in the tested genes; at 45◦C, the molecular changes were associated with an antioxidant response, possibly modulated by anthocyanins. At 55◦C, HSP17a.22, MTL and proline accumulation were evident. After recovery, physiological balance was restored, whereas POX1, HSP10.4 and MTL abundances were suggested to be involved in increased thermotolerance. The data presented here are expected to pinpoint some pathways changes occurring during such stress and further recovery in this particular Mediterranean species. ©</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Almeida, Tânia</style></author><author><style face="normal" font="default" size="100%">Pinto, Glória</style></author><author><style face="normal" font="default" size="100%">Correia, Barbara</style></author><author><style face="normal" font="default" size="100%">Santos, Conceição</style></author><author><style face="normal" font="default" size="100%">Gonçalves, Sónia</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">QsMYB1 expression is modulated in response to heat and drought stresses and during plant recovery in Quercus suber</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Physiology and Biochemistry</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Abiotic stress</style></keyword><keyword><style  face="normal" font="default" size="100%">Adaptation</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Droughts</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene expression</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene Expression Regulation</style></keyword><keyword><style  face="normal" font="default" size="100%">Genes</style></keyword><keyword><style  face="normal" font="default" size="100%">Hot Temperature</style></keyword><keyword><style  face="normal" font="default" size="100%">Physiological</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Bark</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">R2R3-MYB</style></keyword><keyword><style  face="normal" font="default" size="100%">Recovery</style></keyword><keyword><style  face="normal" font="default" size="100%">RNA Splicing</style></keyword><keyword><style  face="normal" font="default" size="100%">Stress</style></keyword><keyword><style  face="normal" font="default" size="100%">Transcription Factors</style></keyword><keyword><style  face="normal" font="default" size="100%">Transcription Factors: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Transcription Factors: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">water</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2013///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/24161757http://www.sciencedirect.com/science/article/pii/S0981942813003537</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">73</style></volume><pages><style face="normal" font="default" size="100%">274 - 281</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Abstract Cork oak is an economically important forest species showing a great tolerance to high temperatures and shortage of water. However, the mechanisms underlying this plasticity are still poorly understood. Among the stress regulators, transcription factors (TFs) are especially important since they can control a wide range of stress-inducible genes, which make them powerful targets for genetic engineering of stress tolerance. Here we evaluated the influence of increasing temperatures (up to 55 °C) or drought (18% field capacity, FC) on the expression profile of an R2R3-MYB transcription factor of cork oak, the QsMYB1. QsMYB1 was previously identified as being preferentially expressed in cork tissues and as having an associated alternative splicing mechanism, which results in two different transcripts (QsMYB1.1 and QsMYB1.2). Expression analysis by reverse transcription quantitative PCR (RT-qPCR) revealed that increasing temperatures led to a gradual down-regulation of QsMYB1 transcripts with more effect on QsMYB1.1 abundance. On the other hand, under drought condition, expression of QsMYB1 variants, mainly the QsMYB1.2, was transiently up-regulated shortly after the stress imposition. Recovery from each stress has also resulted in a differential response by both QsMYB1 transcripts. Several physiological and biochemical parameters (plant water status, chlorophyll fluorescence, lipid peroxidation and proline content) were determined in order to monitor the plant performance under stress and recovery. In conclusion, this report provides the first evidence that QsMYB1 TF may have a putative function in the regulatory network of cork oak response to heat and drought stresses and during plant recovery.</style></abstract><notes><style face="normal" font="default" size="100%">From Duplicate 1 (QsMYB1 expression is modulated in response to heat and drought stresses and during plant recovery in Quercus suber - Almeida, Tânia; Pinto, Glória; Correia, Barbara; Santos, Conceição; Gonçalves, Sónia)From Duplicate 1 (QsMYB1 expression is modulated in response to heat and drought stresses and during plant recovery in Quercus suber - Almeida, Tânia; Pinto, Glória; Correia, Barbara; Santos, Conceição; Gonçalves, Sónia)The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Elsevier Masson SAS&lt;br/&gt;accession-num: 24161757</style></notes></record></records></xml>