<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Alvarez, Rubén</style></author><author><style face="normal" font="default" size="100%">Alvarez, José M.</style></author><author><style face="normal" font="default" size="100%">Humara, Jaime M.</style></author><author><style face="normal" font="default" size="100%">Revilla, Angeles</style></author><author><style face="normal" font="default" size="100%">Ordás, Ricardo J.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Genetic transformation of cork oak (Quercus suber L.) for herbicide resistance.</style></title><secondary-title><style face="normal" font="default" size="100%">Biotechnology letters</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Acetyltransferases</style></keyword><keyword><style  face="normal" font="default" size="100%">Acetyltransferases: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Acetyltransferases: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene Dosage</style></keyword><keyword><style  face="normal" font="default" size="100%">Genomic Instability</style></keyword><keyword><style  face="normal" font="default" size="100%">Herbicide Resistance</style></keyword><keyword><style  face="normal" font="default" size="100%">Herbicides</style></keyword><keyword><style  face="normal" font="default" size="100%">Herbicides: toxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plants, Genetically Modified</style></keyword><keyword><style  face="normal" font="default" size="100%">Plants, Genetically Modified: drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Plants, Genetically Modified: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Plants, Genetically Modified: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Plasmids</style></keyword><keyword><style  face="normal" font="default" size="100%">Promoter Regions, Genetic</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Rhizobium</style></keyword><keyword><style  face="normal" font="default" size="100%">Rhizobium: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Transformation, Genetic</style></keyword><keyword><style  face="normal" font="default" size="100%">Zea mays</style></keyword><keyword><style  face="normal" font="default" size="100%">Zea mays: genetics</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2009</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2009///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/19543858</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">31</style></volume><pages><style face="normal" font="default" size="100%">1477 - 83</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The bar gene was introduced into the cork oak genome. Cork oak embryogenic masses were transformed using the Agrobacterium strain AGL1 which carried the plasmid pBINUbiBar. This vector harbours the genes, nptII and bar, the latter under control of the maize ubiquitin promoter. The transgenic embryogenic lines were cryopreserved. Varying activities of phosphinothricin acetyl transferase were detected among the lines, which carried 1-4 copies of the insert. Molecular and biochemical assays confirmed the stability and expression of the transgenes 3 months after thawing the cultures. These results demonstrate genetic engineering of herbicide tolerance in Quercus spp.</style></abstract><issue><style face="normal" font="default" size="100%">9</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;accession-num: 19543858</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors></contributors><titles><title><style face="normal" font="default" size="100%">Genetic transformation of cork oak (Quercus suber L.) for herbicide resistance.</style></title><secondary-title><style face="normal" font="default" size="100%">Biotechnology letters</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2009</style></year></dates><volume><style face="normal" font="default" size="100%">31</style></volume><pages><style face="normal" font="default" size="100%">1477-83</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The bar gene was introduced into the cork oak genome. Cork oak embryogenic masses were transformed using the Agrobacterium strain AGL1 which carried the plasmid pBINUbiBar. This vector harbours the genes, nptII and bar, the latter under control of the maize ubiquitin promoter. The transgenic embryogenic lines were cryopreserved. Varying activities of phosphinothricin acetyl transferase were detected among the lines, which carried 1-4 copies of the insert. Molecular and biochemical assays confirmed the stability and expression of the transgenes 3 months after thawing the cultures. These results demonstrate genetic engineering of herbicide tolerance in Quercus spp.</style></abstract><accession-num><style face="normal" font="default" size="100%">19543858</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>5</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Alvarez, Rubén</style></author><author><style face="normal" font="default" size="100%">Toribio, Mariano</style></author><author><style face="normal" font="default" size="100%">Cortizo, Millán</style></author><author><style face="normal" font="default" size="100%">Ordás Fernández, Ricardo-Javier</style></author></authors><secondary-authors><author><style face="normal" font="default" size="100%">Wang, K.</style></author></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Cork Oak Trees (Quercus suber L.).</style></title><secondary-title><style face="normal" font="default" size="100%">Methods in molecular biology (Clifton, N.J.)</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">AGL1</style></keyword><keyword><style  face="normal" font="default" size="100%">Agrobacterium tumefaciens</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Fagaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">kanamycin resistance</style></keyword><keyword><style  face="normal" font="default" size="100%">pBINUbiGUSint</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">Somatic embryogenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Tree genetic transformation</style></keyword><keyword><style  face="normal" font="default" size="100%">β-glucuronidase</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2006</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2006///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/17033056</style></url></web-urls></urls><publisher><style face="normal" font="default" size="100%">Humana Press</style></publisher><pub-location><style face="normal" font="default" size="100%">Totowa</style></pub-location><volume><style face="normal" font="default" size="100%">344</style></volume><pages><style face="normal" font="default" size="100%">113 - 123</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">A transformation system for selected mature Quercus suber L. trees using Agrobacterium tumefaciens has been established. Embryos obtained from recurrent proliferating embryogenic masses are inoculated with AGL1 strain harbouring the plasmid pBINUbiGUSint, which carries the nptII and uidA genes. Evidence of stable transgene integration is obtained by polymerase chain reaction for nptII and uidA genes, Southern blotting and expression of the uidA gene. The transgenic embryos are germinated and successfully transferred to soil.</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;periodical: Methods in molecular biology (Clifton, N.J.)&lt;br/&gt;accession-num: 17033056&lt;br/&gt;electronic-resource-num: 10.1385/1-59745-131-2:113</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>7</ref-type><contributors></contributors><titles><title><style face="normal" font="default" size="100%">Cork Oak Trees (Quercus suber L.).</style></title><secondary-title><style face="normal" font="default" size="100%">Methods in molecular biology (Clifton, N.J.)</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2006</style></year></dates><publisher><style face="normal" font="default" size="100%">Humana Press</style></publisher><pub-location><style face="normal" font="default" size="100%">Totowa</style></pub-location><volume><style face="normal" font="default" size="100%">344</style></volume><pages><style face="normal" font="default" size="100%">113-123</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">A transformation system for selected mature Quercus suber L. trees using Agrobacterium tumefaciens has been established. Embryos obtained from recurrent proliferating embryogenic masses are inoculated with AGL1 strain harbouring the plasmid pBINUbiGUSint, which carries the nptII and uidA genes. Evidence of stable transgene integration is obtained by polymerase chain reaction for nptII and uidA genes, Southern blotting and expression of the uidA gene. The transgenic embryos are germinated and successfully transferred to soil.</style></abstract><accession-num><style face="normal" font="default" size="100%">17033056</style></accession-num></record></records></xml>