<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>5</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Álvarez-Fernández, Rubén</style></author><author><style face="normal" font="default" size="100%">Ordás, Ricardo-Javier</style></author></authors><secondary-authors><author><style face="normal" font="default" size="100%">Loyola-Vargas, Víctor M.</style></author><author><style face="normal" font="default" size="100%">Ochoa-Alejo, Neftalí</style></author></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Improved Genetic Transformation of Cork Oak (Quercus suber L.)</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Cell Culture Protocols SE - 28</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">AGL1</style></keyword><keyword><style  face="normal" font="default" size="100%">Agrobacterium tumefaciens</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Fagaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">Herbicide Resistance</style></keyword><keyword><style  face="normal" font="default" size="100%">kanamycin resistance</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">Somatic embryogenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Tree genetic transformation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2012///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1007/978-1-61779-818-4_28</style></url></web-urls></urls><publisher><style face="normal" font="default" size="100%">Humana Press</style></publisher><volume><style face="normal" font="default" size="100%">877</style></volume><pages><style face="normal" font="default" size="100%">385 - 399 LA -- English</style></pages><isbn><style face="normal" font="default" size="100%">978-1-61779-817-7</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">An Agrobacterium-mediated transformation system for selected mature Quercus suber L. trees has been established. Leaf-derived somatic embryos in an early stage of development are inoculated with an AGL1 strain harboring a kanamycin-selectable plasmid carrying the gene of interest. The transformed embryos are induced to germinate and the plantlets transferred to soil. This protocol, from adult cork oak to transformed plantlet, can be completed in about one and a half years. Transformation efficiencies (i.e., percentage of inoculated explants that yield independent transgenic embryogenic lines) vary depending on the cork oak genotype, reaching up to 43%.</style></abstract><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;periodical: Plant Cell Culture Protocols SE - 28&lt;br/&gt;electronic-resource-num: 10.1007/978-1-61779-818-4_28</style></notes></record></records></xml>