<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Evidente, Antonio</style></author><author><style face="normal" font="default" size="100%">Masi, Marco</style></author><author><style face="normal" font="default" size="100%">Linaldeddu, Benedetto T.</style></author><author><style face="normal" font="default" size="100%">Franceschini, Antonio</style></author><author><style face="normal" font="default" size="100%">Scanu, Bruno</style></author><author><style face="normal" font="default" size="100%">Cimmino, Alessio</style></author><author><style face="normal" font="default" size="100%">Andolfi, Anna</style></author><author><style face="normal" font="default" size="100%">Motta, Andrea</style></author><author><style face="normal" font="default" size="100%">Maddau, Lucia</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Afritoxinones A and B, dihydrofuropyran-2-ones produced by Diplodia africana the causal agent of branch dieback on Juniperus phoenicea</style></title><secondary-title><style face="normal" font="default" size="100%">PHYTOCHEMISTRY</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Afritoxinones A and B</style></keyword><keyword><style  face="normal" font="default" size="100%">Botryosphaeriaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">Diplodia africana</style></keyword><keyword><style  face="normal" font="default" size="100%">Juniperus phoenicea</style></keyword><keyword><style  face="normal" font="default" size="100%">Phytotoxins</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2012///</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">77</style></volume><pages><style face="normal" font="default" size="100%">245 - 250</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Two phytotoxic dihydrofuropyran-2-ones, named afritoxinones A and B. were isolated from liquid culture of Diplodia africana. a fungal pathogen responsible for branch dieback of Phoenicean juniper in Italy. Additionally, six others known metabolites were isolated and characterized: oxysporone, sphaeropsidin A, epi-sphaeropsidone, R-(-)-mellein, (3R,4R)-4-hydroxymellein and (3R,4S)-4-hydroxymellein. The structures of afritoxinones A and B were established by spectroscopic and optical methods and determined to be as (3aS{*},6R{*}-,7aS)-6-methoxy-3a,7a-dihydro-3H,6H-furo\{[\}2,3-b]pyran-2-o ne and (3aR{*},6R{*},7aS)-6-methoxy-3a,7a-dihydro-3H,6H-furo\{[\}2,3-b]pyran-2-on e, respectively. The phytotoxic activity of afritoxinones A and B and oxysporone was evaluated on host (Phoenicean juniper) and non-host plant (holm oak, cork oak and tomato) by cutting and leaf puncture assay. Oxysporone proved to be the most phytotoxic compound. This study represents the first report of secondary metabolites produced by D. africana. In addition, the taxonomic implications of secondary metabolites in Botryosphaeriaceae family studies are discussed. (C) 2012 Elsevier Ltd. All rights reserved.</style></abstract><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;pub-location: THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND&lt;br/&gt;publisher: PERGAMON-ELSEVIER SCIENCE LTD</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Andolfi, Anna</style></author><author><style face="normal" font="default" size="100%">Maddau, Lucia</style></author><author><style face="normal" font="default" size="100%">Cimmino, Alessio</style></author><author><style face="normal" font="default" size="100%">Linaldeddu, Benedetto T.</style></author><author><style face="normal" font="default" size="100%">Franceschini, Antonio</style></author><author><style face="normal" font="default" size="100%">Serra, Salvatorica</style></author><author><style face="normal" font="default" size="100%">Basso, Sara</style></author><author><style face="normal" font="default" size="100%">Melck, Dominique</style></author><author><style face="normal" font="default" size="100%">Evidente, Antonio</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cyclobotryoxide, a phytotoxic metabolite produced by the plurivorous pathogen Neofusicoccum australe.</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of natural products</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Ascomycota</style></keyword><keyword><style  face="normal" font="default" size="100%">Ascomycota: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Bicyclo Compounds, Heterocyclic</style></keyword><keyword><style  face="normal" font="default" size="100%">Bicyclo Compounds, Heterocyclic: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Bicyclo Compounds, Heterocyclic: isolation &amp; purif</style></keyword><keyword><style  face="normal" font="default" size="100%">Bicyclo Compounds, Heterocyclic: pharmacology</style></keyword><keyword><style  face="normal" font="default" size="100%">Catechols</style></keyword><keyword><style  face="normal" font="default" size="100%">Cyclohexanones</style></keyword><keyword><style  face="normal" font="default" size="100%">Cyclohexanones: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Cyclohexanones: isolation &amp; purification</style></keyword><keyword><style  face="normal" font="default" size="100%">Cyclohexanones: pharmacology</style></keyword><keyword><style  face="normal" font="default" size="100%">Juniperus</style></keyword><keyword><style  face="normal" font="default" size="100%">Juniperus: microbiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular Structure</style></keyword><keyword><style  face="normal" font="default" size="100%">mycotoxins</style></keyword><keyword><style  face="normal" font="default" size="100%">Mycotoxins: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Mycotoxins: isolation &amp; purification</style></keyword><keyword><style  face="normal" font="default" size="100%">Mycotoxins: pharmacology</style></keyword><keyword><style  face="normal" font="default" size="100%">Nuclear Magnetic Resonance, Biomolecular</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Stereoisomerism</style></keyword><keyword><style  face="normal" font="default" size="100%">Vitis</style></keyword><keyword><style  face="normal" font="default" size="100%">Vitis: drug effects</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2012///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/23046443</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">75</style></volume><pages><style face="normal" font="default" size="100%">1785 - 91</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Two isolates of Neofusicoccum australe belonging to ITS haplotypes H4 and H1 and associated with grapevine cordon dieback and branch dieback of Phoenicean juniper, respectively, have been shown to produce in vitro structurally different secondary metabolites. From the strain BOT48 of N. australe (haplotype H4) a new cyclohexenone oxide, namely, cyclobotryoxide, was isolated together with 3-methylcatechol and tyrosol. Cyclobotryoxide was characterized as (1S,5R,6S)-5-hydroxy-3-methoxy-4-methyl-7-oxabicyclo[4.1.0]hept-3-en-2-one by spectroscopic, optical, and chemical methods. The strain BL24 (haplotype H1) produced tyrosol along with botryosphaerone D and (3S,4S)-3,4,8-trihydroxy-6-methoxy-3,4-dihydro-1(2H)-naphthalenone. The metabolites obtained from both strains were tested at four concentrations on leaves of grapevine cv. Cannonau, holm oak, and cork oak by the leaf puncture assay. Cyclobotryoxide proved to be the most phytotoxic compound. Tyrosol and cyclobotryoxide were also tested on detached grapevine leaves at concentrations of 0.25 and 0.5 mg/mL. Only cyclobotryoxide was found to be active in this bioassay.</style></abstract><issue><style face="normal" font="default" size="100%">10</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;accession-num: 23046443</style></notes></record></records></xml>