<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Bueno, María a</style></author><author><style face="normal" font="default" size="100%">Gomez, Arancha</style></author><author><style face="normal" font="default" size="100%">Sepulveda, Federico</style></author><author><style face="normal" font="default" size="100%">Seguí, José M</style></author><author><style face="normal" font="default" size="100%">Testillano, Pilar S</style></author><author><style face="normal" font="default" size="100%">Manzanera, Jose a</style></author><author><style face="normal" font="default" size="100%">Risueño, Maria-Carmen</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Microspore-derived embryos from Quercus suber anthers mimic zygotic embryos and maintain haploidy in long-term anther culture.</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of plant physiology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cytochemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA-Analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">flow cytometry</style></keyword><keyword><style  face="normal" font="default" size="100%">haploid embryos</style></keyword><keyword><style  face="normal" font="default" size="100%">immunocyto- chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber L.</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2003</style></year></dates><volume><style face="normal" font="default" size="100%">160</style></volume><pages><style face="normal" font="default" size="100%">953-960</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Microspore-derived embryos produced from cork oak anther cultures after long-term incubations (up to 10-12 months) were analysed in order to determine the genetic variability and ploidy level stability, as well as morphology, developmental pattern and cellular organisation. Most of the embryos from long-term anther cultures were haploid (90.7%), corresponding to their microspore origin. The presence of a low percentage of diploid embryos (7.4%) was observed. Microsatellite analysis of haploid embryos, indicated different microspores origins of the same anther. In the diploid embryos, homozygosity for different alleles was detected from anther wall tissues, excluding the possibility of clonal origin. The maintenance of a high proportion of haploid embryos, in long-term anther cultures, is similar in percentage to that reported in embryos originating after 20 days of plating (Bueno et al. 1997). This suggests that no significant alterations in the ploidy level occurred during long incubations (up to 12 months). These results suggest that ploidy changes are rare in this in vitro system, and do not significantly increase during long-term cultures. Microscopical studies of the microspore embryos in various stages revealed a healthy and well developed anatomy with no aberrant or chimeric structures. The general morphology of embryos appearing at different times after plating, looked similar to that of earlier embryos, as well as the zygotic embryos, indicating that they represent high quality material for cork oak breeding.</style></abstract><accession-num><style face="normal" font="default" size="100%">12964871</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Bueno, María a</style></author><author><style face="normal" font="default" size="100%">Gomez, Arancha</style></author><author><style face="normal" font="default" size="100%">Sepulveda, Federico</style></author><author><style face="normal" font="default" size="100%">Seguí, José M.</style></author><author><style face="normal" font="default" size="100%">Testillano, Pilar S.</style></author><author><style face="normal" font="default" size="100%">Manzanera, Jose a</style></author><author><style face="normal" font="default" size="100%">Risueño, Maria-Carmen</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Microspore-derived embryos from Quercus suber anthers mimic zygotic embryos and maintain haploidy in long-term anther culture.</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of plant physiology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cytochemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA-Analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">flow cytometry</style></keyword><keyword><style  face="normal" font="default" size="100%">haploid embryos</style></keyword><keyword><style  face="normal" font="default" size="100%">immunocyto- chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber L.</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2003</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2003///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/12964871</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">160</style></volume><pages><style face="normal" font="default" size="100%">953 - 960</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Microspore-derived embryos produced from cork oak anther cultures after long-term incubations (up to 10-12 months) were analysed in order to determine the genetic variability and ploidy level stability, as well as morphology, developmental pattern and cellular organisation. Most of the embryos from long-term anther cultures were haploid (90.7%), corresponding to their microspore origin. The presence of a low percentage of diploid embryos (7.4%) was observed. Microsatellite analysis of haploid embryos, indicated different microspores origins of the same anther. In the diploid embryos, homozygosity for different alleles was detected from anther wall tissues, excluding the possibility of clonal origin. The maintenance of a high proportion of haploid embryos, in long-term anther cultures, is similar in percentage to that reported in embryos originating after 20 days of plating (Bueno et al. 1997). This suggests that no significant alterations in the ploidy level occurred during long incubations (up to 12 months). These results suggest that ploidy changes are rare in this in vitro system, and do not significantly increase during long-term cultures. Microscopical studies of the microspore embryos in various stages revealed a healthy and well developed anatomy with no aberrant or chimeric structures. The general morphology of embryos appearing at different times after plating, looked similar to that of earlier embryos, as well as the zygotic embryos, indicating that they represent high quality material for cork oak breeding.</style></abstract><issue><style face="normal" font="default" size="100%">8</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;accession-num: 12964871</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Bueno, Maria Angeles</style></author><author><style face="normal" font="default" size="100%">Gomez, Arancha</style></author><author><style face="normal" font="default" size="100%">Boscaiu, Monica</style></author><author><style face="normal" font="default" size="100%">Manzanera, Jose Antonio</style></author><author><style face="normal" font="default" size="100%">Vicente, Oscar</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Stress-induced formation of haploid plants through anther culture in cork oak (Quercus suber)</style></title><secondary-title><style face="normal" font="default" size="100%">Physiologia Plantarum</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">anther culture</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">flow cytometry</style></keyword><keyword><style  face="normal" font="default" size="100%">Haploid plants</style></keyword><keyword><style  face="normal" font="default" size="100%">heat shock</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">starvation</style></keyword><keyword><style  face="normal" font="default" size="100%">Stress</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1997</style></year><pub-dates><date><style  face="normal" font="default" size="100%">1997///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://doi.wiley.com/10.1111/j.1399-3054.1997.tb05421.x</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">99</style></volume><pages><style face="normal" font="default" size="100%">335 - 341</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Induetion of haploid embryos and regeneration of plantlets have been obtained, for the first time, in cork oak (Quercus suber L.) by combining a starvation treatment in anther culture with a mild heat shock at 33°C for 5 days, followed by eulture at 25°C in a simple agar medium without growth regulators. The same conditions had been shown previously to be optimal for embryogenic induction in isolated microspore cultures of' several model species such as tobacco and wheat. These results support the notion that stress, particularly sucrose starvation, a heat shock or a combination of both treatments could be the major and general signal responsible for the inhibition of normal gametophytie development of the mierospores and for the induetion of the alternative embryogenic pathway. A similar approach may be used for the production of haploid and doubled haploids for plant breeding in other species that, like most forest trees, are still recalcitrant in anther culture.</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Bueno, Maria Angeles</style></author><author><style face="normal" font="default" size="100%">Gomez, Arancha</style></author><author><style face="normal" font="default" size="100%">Boscaiu, Monica</style></author><author><style face="normal" font="default" size="100%">Manzanera, Jose Antonio</style></author><author><style face="normal" font="default" size="100%">Vicente, Oscar</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Stress-induced formation of haploid plants through anther culture in cork oak (Quercus suber)</style></title><secondary-title><style face="normal" font="default" size="100%">Physiologia Plantarum</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">anther culture</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">flow cytometry</style></keyword><keyword><style  face="normal" font="default" size="100%">Haploid plants</style></keyword><keyword><style  face="normal" font="default" size="100%">heat shock</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">starvation</style></keyword><keyword><style  face="normal" font="default" size="100%">Stress</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1997</style></year></dates><volume><style face="normal" font="default" size="100%">99</style></volume><pages><style face="normal" font="default" size="100%">335-341</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Induetion of haploid embryos and regeneration of plantlets have been obtained, for the first time, in cork oak (Quercus suber L.) by combining a starvation treatment in anther culture with a mild heat shock at 33°C for 5 days, followed by eulture at 25°C in a simple agar medium without growth regulators. The same conditions had been shown previously to be optimal for embryogenic induction in isolated microspore cultures of' several model species such as tobacco and wheat. These results support the notion that stress, particularly sucrose starvation, a heat shock or a combination of both treatments could be the major and general signal responsible for the inhibition of normal gametophytie development of the mierospores and for the induetion of the alternative embryogenic pathway. A similar approach may be used for the production of haploid and doubled haploids for plant breeding in other species that, like most forest trees, are still recalcitrant in anther culture.</style></abstract></record></records></xml>