<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Coelho, A. C.</style></author><author><style face="normal" font="default" size="100%">Cravador, A.</style></author><author><style face="normal" font="default" size="100%">Bollen, A.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Highly specific and sensitive non-radioactive molecular identification of Phytophthora cinnamomi</style></title><secondary-title><style face="normal" font="default" size="100%">Mycological Research</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cork oak soils</style></keyword><keyword><style  face="normal" font="default" size="100%">Iidentification method</style></keyword><keyword><style  face="normal" font="default" size="100%">phytophthora cinnamomi</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1997</style></year><pub-dates><date><style  face="normal" font="default" size="100%">1997///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.sciencedirect.com/science/article/pii/S0953756208608199</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">101</style></volume><pages><style face="normal" font="default" size="100%">1499 - 1507</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">In response to the need for a faster, more reliable method for identifying Phytophthora cinnamomi in cork oak soils in Portugal, a simple, fast, sensitive molecular identi®cation method is described. It is based on a colorimetric assay which involves an oligonucleotide capture probe covalently immobilised on microtitration wells, a multi-biotinylated oligonucleotide detection probe and the PCR-ampli®ed target DNA. The target DNA is a 349 bp DNA fragment partially covering the 3«-translated and 3«- untranslated regions of the cinnamomin gene. When the speci®city of the PCR reaction was evaluated in vitro using isolates of P. cinnamomi and eight other Phytophthora species, including the related P. cambivora, it was speci®c to P. cinnamomi. When 30 isolates of P. cinnamomi from oak roots in southern Portugal were assayed, 26 gave a strong positive response. The assay has a sensitivity of about 2±5 genome equivalents of P. cinnamomi. The reason for the negative response of four isolates remains unclear.</style></abstract><issue><style face="normal" font="default" size="100%">12</style></issue></record></records></xml>