<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Neves, Dina</style></author><author><style face="normal" font="default" size="100%">Caetano, Paula</style></author><author><style face="normal" font="default" size="100%">Oliveira, Joana</style></author><author><style face="normal" font="default" size="100%">Maia, Cristiana</style></author><author><style face="normal" font="default" size="100%">Horta, Marília</style></author><author><style face="normal" font="default" size="100%">Sousa, Nelson</style></author><author><style face="normal" font="default" size="100%">Salgado, Marco</style></author><author><style face="normal" font="default" size="100%">Dionisio, Lidia</style></author><author><style face="normal" font="default" size="100%">Magan, Naresh</style></author><author><style face="normal" font="default" size="100%">Cravador, Alfredo</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Anti-Phytophthora cinnamomi activity of Phlomis purpurea plant and root extracts</style></title><secondary-title><style face="normal" font="default" size="100%">EUROPEAN JOURNAL OF PLANT PATHOLOGY</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Biocontrol</style></keyword><keyword><style  face="normal" font="default" size="100%">Disease reduction</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">root rot</style></keyword><keyword><style  face="normal" font="default" size="100%">Zoospores</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year></dates><publisher><style face="normal" font="default" size="100%">SPRINGER</style></publisher><pub-location><style face="normal" font="default" size="100%">VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS</style></pub-location><volume><style face="normal" font="default" size="100%">138</style></volume><pages><style face="normal" font="default" size="100%">835-846</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Phlomis purpurea (Lamiaceae), found in Quercus suber and Quercus ilex ssp. rotundifolia forest habitats in southern Portugal, is a non-host for the oomycete Phytophthora cinnamomi, the main biotic factor involved in cork oak and holm oak decline in the Iberian Peninsula. The effect of P. purpurea crude ethanol root extract was evaluated in vitro on P. cinnamomi mycelial growth, sporangial production, zoospore release and germination as well as on chlamydospore production and viability. The protection of cork oak against infection by the pathogen was also evaluated in planta. At 10 mg ml(-1), in vitro inhibition of the pathogen structures was 85-100 %. In addition, P. purpurea plants were shown to protect Q. suber and Q. ilex from P. cinnamomi infection and to reduce the inoculum potential in glasshouse trials, indicating the ability to reduce root infection by the pathogen. The results suggest that P. purpurea has the potential to reduce disease spread and that their root extracts could provide candidate substances for control of the important pathogen, P. cinnamomi.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Pereira-Leal, José B</style></author><author><style face="normal" font="default" size="100%">Abreu, Isabel a</style></author><author><style face="normal" font="default" size="100%">Alabaça, Cláudia S</style></author><author><style face="normal" font="default" size="100%">Almeida, Maria Helena</style></author><author><style face="normal" font="default" size="100%">Almeida, Paulo</style></author><author><style face="normal" font="default" size="100%">Almeida, Tânia</style></author><author><style face="normal" font="default" size="100%">Amorim, Maria Isabel</style></author><author><style face="normal" font="default" size="100%">Araújo, Susana</style></author><author><style face="normal" font="default" size="100%">Azevedo, Herlânder</style></author><author><style face="normal" font="default" size="100%">Badia, Aleix</style></author><author><style face="normal" font="default" size="100%">Batista, Dora</style></author><author><style face="normal" font="default" size="100%">Bohn, Andreas</style></author><author><style face="normal" font="default" size="100%">Capote, Tiago</style></author><author><style face="normal" font="default" size="100%">Carrasquinho, Isabel</style></author><author><style face="normal" font="default" size="100%">Chaves, Inês</style></author><author><style face="normal" font="default" size="100%">Coelho, Ana Cristina</style></author><author><style face="normal" font="default" size="100%">Costa, Maria Manuela Ribeiro</style></author><author><style face="normal" font="default" size="100%">Costa, Rita</style></author><author><style face="normal" font="default" size="100%">Cravador, Alfredo</style></author><author><style face="normal" font="default" size="100%">Egas, Conceição</style></author><author><style face="normal" font="default" size="100%">Faro, Carlos</style></author><author><style face="normal" font="default" size="100%">Fortes, Ana M</style></author><author><style face="normal" font="default" size="100%">Fortunato, Ana S</style></author><author><style face="normal" font="default" size="100%">Gaspar, Maria João</style></author><author><style face="normal" font="default" size="100%">Gonçalves, Sónia</style></author><author><style face="normal" font="default" size="100%">Graça, José</style></author><author><style face="normal" font="default" size="100%">Horta, Marília</style></author><author><style face="normal" font="default" size="100%">Inácio, Vera</style></author><author><style face="normal" font="default" size="100%">Leitão, José M</style></author><author><style face="normal" font="default" size="100%">Lino-Neto, Teresa</style></author><author><style face="normal" font="default" size="100%">Marum, Liliana</style></author><author><style face="normal" font="default" size="100%">Matos, José</style></author><author><style face="normal" font="default" size="100%">Mendonça, Diogo</style></author><author><style face="normal" font="default" size="100%">Miguel, Andreia</style></author><author><style face="normal" font="default" size="100%">Miguel, Célia M</style></author><author><style face="normal" font="default" size="100%">Morais-Cecílio, Leonor</style></author><author><style face="normal" font="default" size="100%">Neves, Isabel</style></author><author><style face="normal" font="default" size="100%">Nóbrega, Filomena</style></author><author><style face="normal" font="default" size="100%">Oliveira, Maria Margarida</style></author><author><style face="normal" font="default" size="100%">Oliveira, Rute</style></author><author><style face="normal" font="default" size="100%">Pais, Maria Salomé</style></author><author><style face="normal" font="default" size="100%">Paiva, Jorge a</style></author><author><style face="normal" font="default" size="100%">Paulo, Octávio S</style></author><author><style face="normal" font="default" size="100%">Pinheiro, Miguel</style></author><author><style face="normal" font="default" size="100%">Raimundo, João Ap</style></author><author><style face="normal" font="default" size="100%">Ramalho, José C</style></author><author><style face="normal" font="default" size="100%">Ribeiro, Ana I</style></author><author><style face="normal" font="default" size="100%">Ribeiro, Teresa</style></author><author><style face="normal" font="default" size="100%">Rocheta, Margarida</style></author><author><style face="normal" font="default" size="100%">Rodrigues, Ana Isabel</style></author><author><style face="normal" font="default" size="100%">Rodrigues, José C</style></author><author><style face="normal" font="default" size="100%">Saibo, Nelson Jm</style></author><author><style face="normal" font="default" size="100%">Santo, Tatiana E</style></author><author><style face="normal" font="default" size="100%">Santos, Ana Margarida</style></author><author><style face="normal" font="default" size="100%">Sá-Pereira, Paula</style></author><author><style face="normal" font="default" size="100%">Sebastiana, Mónica</style></author><author><style face="normal" font="default" size="100%">Simões, Fernanda</style></author><author><style face="normal" font="default" size="100%">Sobral, Rómulo S</style></author><author><style face="normal" font="default" size="100%">Tavares, Rui</style></author><author><style face="normal" font="default" size="100%">Teixeira, Rita</style></author><author><style face="normal" font="default" size="100%">Varela, Carolina</style></author><author><style face="normal" font="default" size="100%">Veloso, Maria Manuela</style></author><author><style face="normal" font="default" size="100%">Ricardo, Cândido Pp</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A comprehensive assessment of the transcriptome of cork oak (Quercus suber) through EST sequencing.</style></title><secondary-title><style face="normal" font="default" size="100%">BMC genomics</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">EST sequencing</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">transcriptome</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year></dates><volume><style face="normal" font="default" size="100%">15</style></volume><pages><style face="normal" font="default" size="100%">371</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">BACKGROUND: Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic information. Cork oak forests are of great ecological importance and represent a major economic and social resource in Southern Europe and Northern Africa. However, global warming is threatening the cork oak forests by imposing thermal, hydric and many types of novel biotic stresses. Despite the economic and social value of the Q. suber species, few genomic resources have been developed, useful for biotechnological applications and improved forest management. RESULTS: We generated in excess of 7 million sequence reads, by pyrosequencing 21 normalized cDNA libraries derived from multiple Q. suber tissues and organs, developmental stages and physiological conditions. We deployed a stringent sequence processing and assembly pipeline that resulted in the identification of ~159,000 unigenes. These were annotated according to their similarity to known plant genes, to known Interpro domains, GO classes and E.C. numbers. The phylogenetic extent of this ESTs set was investigated, and we found that cork oak revealed a significant new gene space that is not covered by other model species or EST sequencing projects. The raw data, as well as the full annotated assembly, are now available to the community in a dedicated web portal at http://www.corkoakdb.org. CONCLUSIONS: This genomic resource represents the first trancriptome study in a cork producing species. It can be explored to develop new tools and approaches to understand stress responses and developmental processes in forest trees, as well as the molecular cascades underlying cork differentiation and disease response.</style></abstract><accession-num><style face="normal" font="default" size="100%">24885229</style></accession-num><notes><style face="normal" font="default" size="100%">APS</style></notes><research-notes><style face="normal" font="default" size="100%">APS</style></research-notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Medeira, Clara</style></author><author><style face="normal" font="default" size="100%">Quartin, Virgínia</style></author><author><style face="normal" font="default" size="100%">Maia, Isabel</style></author><author><style face="normal" font="default" size="100%">Diniz, Inês</style></author><author><style face="normal" font="default" size="100%">Matos, Mª Céu</style></author><author><style face="normal" font="default" size="100%">Semedo, José Nobre</style></author><author><style face="normal" font="default" size="100%">Scotti-Campos, Paula</style></author><author><style face="normal" font="default" size="100%">Ramalho, José C</style></author><author><style face="normal" font="default" size="100%">Pais, Isabel P</style></author><author><style face="normal" font="default" size="100%">Ramos, Paula</style></author><author><style face="normal" font="default" size="100%">Melo, Elvira</style></author><author><style face="normal" font="default" size="100%">Leitão, António E</style></author><author><style face="normal" font="default" size="100%">Cravador, Alfredo</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cryptogein and capsicein promote defence responses in Quercus suber against Phytophthora cinnamomi infection</style></title><secondary-title><style face="normal" font="default" size="100%">European Journal of Plant Pathology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">cytology</style></keyword><keyword><style  face="normal" font="default" size="100%">decline disease</style></keyword><keyword><style  face="normal" font="default" size="100%">fluorescence</style></keyword><keyword><style  face="normal" font="default" size="100%">leaf gas exchanges</style></keyword><keyword><style  face="normal" font="default" size="100%">Membrane Lipids</style></keyword><keyword><style  face="normal" font="default" size="100%">photosynthetic pigments</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year></dates><volume><style face="normal" font="default" size="100%">134</style></volume><pages><style face="normal" font="default" size="100%">145-159</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The decline of cork oak (Quercus suber) stands in Iberian Peninsula is associated with infection by Phytophthora cinnamomi. Most Phytophthora species secrete elicitins, which can enhance defence reactions against some pathogens. Here cytological and physiological effects of the elicitins cryptogein and capsicein on cork oak root infection by P. cinnamomi were evaluated. The progression of the pathogen in root tissue and its effects on total fatty acid (TFA) composition of roots and leaves were analysed in seedlings. Net photosynthesis (Pn), stomatal conductance (gs ), chlorophyll a fluorescence measurements (quantum yield of linear electron transport ϕe , photochemical quenching qP, non-photochemical quenching NPQ) and carotenoid determinations were carried out in well established (4 months) plants. In elicitin-treated roots, 2 days after inoculation, the pathogen which presented loss of viability and membrane degradation was mainly restricted to the intercellular spaces of the cortical parenchyma, and did not reach the vascular cylinder. Electron dense materials accumulated in the intercellular spaces of the cortex next to disorganized hyphae, suggested to be related with defence reactions. Cryptogein (or its interaction with P. cinnamomi) induced enhanced lipid synthesis in leaves, which may contribute to preserve membrane stability. P. cinnamomi decreased Pn , gs , ϕe , and qP, whereas elicitintreated plants displayed values similar to controls. Overall, the results indicated a resistance response of cork oak against this oomycete, induced by the elicitins.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>47</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Coelho, Ana Cristina</style></author><author><style face="normal" font="default" size="100%">Ebadzad, Ghazal</style></author><author><style face="normal" font="default" size="100%">Cravador, Alfredo</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">New Zealand Journal of Forestry Science</style></title><secondary-title><style face="normal" font="default" size="100%">Fifth Meeting of the IUFRO Working Party S07-02-09, Phytophthora Diseases in Forests and Natural Ecosystems</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">cdna-aflp</style></keyword><keyword><style  face="normal" font="default" size="100%">defense-response pathways</style></keyword><keyword><style  face="normal" font="default" size="100%">oak/oomycete interaction</style></keyword><keyword><style  face="normal" font="default" size="100%">pathogenesis related proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">phytophthora cinnamomi</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2011///</style></date></pub-dates></dates><publisher><style face="normal" font="default" size="100%">New Zealand Journal of Forestry Science</style></publisher><volume><style face="normal" font="default" size="100%">0134</style></volume><pages><style face="normal" font="default" size="100%">143 - 157</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;periodical: Fifth Meeting of the IUFRO Working Party S07-02-09, Phytophthora Diseases in Forests and Natural Ecosystems&lt;br/&gt;pub-location: Auckland and Rotorua, New Zealand</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Horta, Marília</style></author><author><style face="normal" font="default" size="100%">Sousa, Nelson</style></author><author><style face="normal" font="default" size="100%">Coelho, Ana C.</style></author><author><style face="normal" font="default" size="100%">Neves, Dina</style></author><author><style face="normal" font="default" size="100%">Cravador, Alfredo</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In vitro and in vivo quantification of elicitin expression in Phytophthora cinnamomi</style></title><secondary-title><style face="normal" font="default" size="100%">Physiological and Molecular Plant Pathology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Elicitin expression</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene silencing</style></keyword><keyword><style  face="normal" font="default" size="100%">Pathogenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">phytophthora cinnamomi</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Real time RT-PCR</style></keyword><keyword><style  face="normal" font="default" size="100%">Transformation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2008</style></year></dates><volume><style face="normal" font="default" size="100%">73</style></volume><pages><style face="normal" font="default" size="100%">48-57</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The differential expression of four Phytophthora cinnamomi elicitin genes was analysed by Real Time RT-PCR. In in vitro cultures, the a-cinnamomin gene showed the highest level of expression, the b-cinnamomin gene (b-cin) was the most inducible, and the HAE transcripts were in low abundance. Transcription of all the elicitins was active during the active growth of the pathogen when infecting cork oak (Quercus suber) roots, and as host colonization progressed, the level of b-cin expression fell, while that of a-cin rose. In an antisense transgenic strain, the silencing of b-cin also negatively affected the expression of other elicitin genes in the cluster. The reduced in planta growth of the b-cin knock-out is related to the altered pattern of elicitin gene expression, supporting the idea that one of the functions of elicitins is related, directly or indirectly, with pathogenesis</style></abstract></record></records></xml>