<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Garcia-Martin, G.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Effect of exogenous ABA on embryo maturation and quantification of endogenous levels of ABA and IAA in Quercus suber somatic embryos</style></title><secondary-title><style face="normal" font="default" size="100%">Plant cell, tissue and …</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">endogenous plant growth regulators</style></keyword><keyword><style  face="normal" font="default" size="100%">GC-MS</style></keyword><keyword><style  face="normal" font="default" size="100%">hplc</style></keyword><keyword><style  face="normal" font="default" size="100%">in vitro</style></keyword><keyword><style  face="normal" font="default" size="100%">somatic embryo</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2005</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2005///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.springerlink.com/index/U76R638228070M15.pdf</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">80</style></volume><pages><style face="normal" font="default" size="100%">171 - 177</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Knowledge of the relationship between indole-3-acetic acid (IAA) and abscisic acid (ABA) is relevant to control the development and the maturation of cork oak (Quercus suber L.) somatic embryos. The addition of 1 lM ABA to the culture medium signiﬁcantly promoted somatic embryo maturation and increased both fresh and dry matter without aﬀecting the relative water content. This eﬀect was parallel to the pattern of variation observed in the endogenous ABA level, which increased from the immature to the mature stage. Endogenous ABA content during the occurrence of secondary embryogenesis was similar to that of the immature stage, showing that embryos with lower ABA levels produced secondary embryos. In contrast, IAA showed the highest concentration during early embryo development and decreased afterwards. Only in somatic embryos subjected to 1-week desiccation followed by stratiﬁcation at 4 C for 2 weeks, was a moderate increment of endogenous IAA content observed. IAA and ABA showed opposite levels during the development and maturation of cork oak somatic embryos and characterised speciﬁc stages of the embryonic development.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>47</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Garcia-Martin, G.</style></author><author><style face="normal" font="default" size="100%">Fernandez-Galiano, E.</style></author><author><style face="normal" font="default" size="100%">Mauri, P. V.</style></author><author><style face="normal" font="default" size="100%">Somática, Embriogénesis</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Multiplicación vegetativa de alcornoque mediante embriogénesis somática.</style></title><secondary-title><style face="normal" font="default" size="100%">II Congreso Forestal Español</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">ABA</style></keyword><keyword><style  face="normal" font="default" size="100%">BaP</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">germination</style></keyword><keyword><style  face="normal" font="default" size="100%">liquid culture</style></keyword><keyword><style  face="normal" font="default" size="100%">maturation</style></keyword><keyword><style  face="normal" font="default" size="100%">somatic embryos</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1997</style></year><pub-dates><date><style  face="normal" font="default" size="100%">1997///</style></date></pub-dates></dates><pages><style face="normal" font="default" size="100%">249 - 252</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">In order to obtain cork oak clones from selected individuals, somatic embryogenesis was induced in leaves. Somatic embryos can be continuously subcultured with high proliferation rates. This culture system has sorne limiting factors: low percentage of mature embryos obtained, low rate of shoot emergence during germination and high labour and time consumed. Exogenous addition of ABA (l0-6M) concentration during proliferation increased maturation rateo In the same way addition of. BAP (0,001 mg/l) had a positive effect on shoot production. With the aim to obtain a first approach to automation the best culture media for both culture cycles of maturation in liquid medium were determined.</style></abstract><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;periodical: II Congreso Forestal Español&lt;br/&gt;pub-location: Pamplona</style></notes></record></records></xml>