<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Coelho, A. C.</style></author><author><style face="normal" font="default" size="100%">Horta, M.</style></author><author><style face="normal" font="default" size="100%">Neves, D.</style></author><author><style face="normal" font="default" size="100%">Cravador, A.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Involvement of a cinnamyl alcohol dehydrogenase of Quercus suber in the defence response to infection by Phytophthora cinnamomi</style></title><secondary-title><style face="normal" font="default" size="100%">Physiological and Molecular Plant Pathology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">cinnamyl alcohol dehydrogenase</style></keyword><keyword><style  face="normal" font="default" size="100%">defence response</style></keyword><keyword><style  face="normal" font="default" size="100%">oak tree</style></keyword><keyword><style  face="normal" font="default" size="100%">phytophthora cinnamomi</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2006</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2006///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://linkinghub.elsevier.com/retrieve/pii/S0885576507000057</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">69</style></volume><pages><style face="normal" font="default" size="100%">62 - 72</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">A gene encoding a potential NADPH-dependent cinnamyl alcohol dehydrogenase (QsCAD1) (GenBank accession no: AY362455) was identiﬁed in Quercus suber (cork oak). Its complete cDNA sequence was obtained by RACE-PCR, starting from total RNA extracted from roots of seedlings of Q. suber, infected with Phytophthora cinnamomi, the causal agent of the decline and sudden death of Q. suber and Quercus ilex subsp. rotundifolia in the Iberian Peninsula. Sequence information to perform the RACE-PCR was acquired from a polymorphic fragment (C9), speciﬁcally identiﬁed by cDNA-AFLP, in leaves of epicormic shoots of a cork oak tree that suffered sudden death. RT-PCR and hybridization analysis showed that the QsCAD1 gene is up-regulated in root seedlings of Q. suber infected with P. cinnamomi. QsCAD1 has a high structural homology with VR-ERE (Vigna radiata), an enzyme that detoxiﬁes eutypine (produced by Eutypa lata, the causal agent of eutypa dieback of grapevines), to eutypinol, and with QrCAD1 (Q. ilex subsp. rotundifolia), EgCAD1 (Eucalyptus gunnii), MdCAD1 (Malus x domestica). Taken together, these results suggest that these enzymes, and namely QsCAD1 belong to a new group of CAD potentially involved in deactivation of toxins produced by phytopathogens.</style></abstract><issue><style face="normal" font="default" size="100%">1-3</style></issue></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Coelho, A. C.</style></author><author><style face="normal" font="default" size="100%">Cravador, A.</style></author><author><style face="normal" font="default" size="100%">Bollen, A.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Highly specific and sensitive non-radioactive molecular identification of Phytophthora cinnamomi</style></title><secondary-title><style face="normal" font="default" size="100%">Mycological Research</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cork oak soils</style></keyword><keyword><style  face="normal" font="default" size="100%">Iidentification method</style></keyword><keyword><style  face="normal" font="default" size="100%">phytophthora cinnamomi</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1997</style></year><pub-dates><date><style  face="normal" font="default" size="100%">1997///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.sciencedirect.com/science/article/pii/S0953756208608199</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">101</style></volume><pages><style face="normal" font="default" size="100%">1499 - 1507</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">In response to the need for a faster, more reliable method for identifying Phytophthora cinnamomi in cork oak soils in Portugal, a simple, fast, sensitive molecular identi®cation method is described. It is based on a colorimetric assay which involves an oligonucleotide capture probe covalently immobilised on microtitration wells, a multi-biotinylated oligonucleotide detection probe and the PCR-ampli®ed target DNA. The target DNA is a 349 bp DNA fragment partially covering the 3«-translated and 3«- untranslated regions of the cinnamomin gene. When the speci®city of the PCR reaction was evaluated in vitro using isolates of P. cinnamomi and eight other Phytophthora species, including the related P. cambivora, it was speci®c to P. cinnamomi. When 30 isolates of P. cinnamomi from oak roots in southern Portugal were assayed, 26 gave a strong positive response. The assay has a sensitivity of about 2±5 genome equivalents of P. cinnamomi. The reason for the negative response of four isolates remains unclear.</style></abstract><issue><style face="normal" font="default" size="100%">12</style></issue></record></records></xml>