<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Rico, L</style></author><author><style face="normal" font="default" size="100%">Ogaya, R</style></author><author><style face="normal" font="default" size="100%">Barbeta, A</style></author><author><style face="normal" font="default" size="100%">Penuelas, J</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Changes in DNA methylation fingerprint of Quercus ilex trees in response to experimental field drought simulating projected climate change</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Biology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">climate change</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA methylation</style></keyword><keyword><style  face="normal" font="default" size="100%">Drought</style></keyword><keyword><style  face="normal" font="default" size="100%">methylation-sensitive amplified polymorphism</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year></dates><volume><style face="normal" font="default" size="100%">16</style></volume><pages><style face="normal" font="default" size="100%">419-427</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Rapid genetic changes in plants have been reported in response to current climate change. We assessed the capacity of trees in a natural forest to produce rapid acclimation responses based on epigenetic modifications. We analysed natural populations of Quercus ilex, the dominant tree species of Mediterranean forests, using the methylation-sensitive amplified polymorphism (MSAP) technique to assess patterns and levels of methylation in individuals from unstressed forest plots and from plots experimentally exposed to drought for 12 years at levels projected for the coming decades. The percentage of hypermethylated loci increased, and the percentage of fully methylated loci clearly decreased in plants exposed to drought. Multivariate analyses exploring the status of methylation at MSAP loci also showed clear differentiation depending on stress. The PCA scores for the MSAP profiles clearly separated the genetic from the epigenetic structure, and also significantly separated the samples within each group in response to drought. Changes in DNA methylation highlight the large capacity of plants to rapidly acclimate to changing environmental conditions, including trees with long life spans, and our results demonstrate those changes. These changes, although unable to prevent the decreased growth and higher mortality associated with this experimental drought, occurred together with a dampening in such decreases as the long-term treatment progressed.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Rico, L</style></author><author><style face="normal" font="default" size="100%">Ogaya, R</style></author><author><style face="normal" font="default" size="100%">Terradas, J</style></author><author><style face="normal" font="default" size="100%">Penuelas, J</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Community structures of N-2-fixing bacteria associated with the phyllosphere of a Holm oak forest and their response to drought</style></title><secondary-title><style face="normal" font="default" size="100%">PLANT BIOLOGY</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Bacterial TRF richness</style></keyword><keyword><style  face="normal" font="default" size="100%">diazotrophic community</style></keyword><keyword><style  face="normal" font="default" size="100%">Drought</style></keyword><keyword><style  face="normal" font="default" size="100%">foliar phyllosphere</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">t-RFLP</style></keyword><keyword><style  face="normal" font="default" size="100%">Trees</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year></dates><publisher><style face="normal" font="default" size="100%">WILEY-BLACKWELL</style></publisher><pub-location><style face="normal" font="default" size="100%">111 RIVER ST, HOBOKEN 07030-5774, NJ USA</style></pub-location><volume><style face="normal" font="default" size="100%">16</style></volume><pages><style face="normal" font="default" size="100%">586-593</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Biological nitrogen (N) fixation is a key pathway in terrestrial ecosystems and is therefore critical for understanding the responses of ecosystems to global environmental changes. The free-living diazotrophic community is distributed along the canopy-to-soil profile, but the ecological significance of epiphyllic N-2 fixers, despite their functional relevance, on plant foliar surfaces remains very poorly understood compared with the N-2-fixing community in forest litter and soils. We assessed the community structure of N-2 fixers and overall bacteria by genetic fingerprinting (t-RFLP) to explore the seasonal successional patterns of the microbial community in the natural phyllosphere of a Holm oak (Quercus ilex) forest submitted to 12-year field experiment of rain exclusion mimicking the conditions of drought projected for the coming decades. Leaves of Holm oak were analysed in different seasons over a period of 1.5years. The bacterial community of the phyllosphere did not correspond to the surrounding soil biome in the same area. These analyses provided field evidence for the presence of free-living diazotrophs associated with the tissues of leaves of Holm oak, the dominant tree species of many Mediterranean forests. The results also revealed that the community composition is affected seasonally and inter-annually by the environment, and that the composition shifts in response to climate change. Drought treatment increased the richness of the epiphyllic microbial community, especially during the summer. These changes were associated with higher C:N ratios of leaves observed in response to drought in semiarid areas. This epiphyllic microbiota that can potentially fix N-2 extends the capacity of plants to adapt to the environment.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Penuelas, J</style></author><author><style face="normal" font="default" size="100%">Rico, L</style></author><author><style face="normal" font="default" size="100%">Ogaya, R</style></author><author><style face="normal" font="default" size="100%">Jump, A S</style></author><author><style face="normal" font="default" size="100%">Terradas, J</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Summer season and long-term drought increase the richness of bacteria and fungi in the foliar phyllosphere of Quercus ilex in a mixed Mediterranean forest</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Biology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Bacteria</style></keyword><keyword><style  face="normal" font="default" size="100%">Bacteria: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Bacteria: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Bacterial</style></keyword><keyword><style  face="normal" font="default" size="100%">Bacterial TRF richness</style></keyword><keyword><style  face="normal" font="default" size="100%">Bacterial: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Biodiversity</style></keyword><keyword><style  face="normal" font="default" size="100%">colonisation time</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Drought</style></keyword><keyword><style  face="normal" font="default" size="100%">Droughts</style></keyword><keyword><style  face="normal" font="default" size="100%">endophytes</style></keyword><keyword><style  face="normal" font="default" size="100%">epiphytes</style></keyword><keyword><style  face="normal" font="default" size="100%">foliar and epiphytic microbial diversity</style></keyword><keyword><style  face="normal" font="default" size="100%">foliar phyllosphere</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungal</style></keyword><keyword><style  face="normal" font="default" size="100%">fungal TRF richness</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungal: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">fungi</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungi: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungi: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Mediterranean Region</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: microbiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Polymorphism</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: microbiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Restriction Fragment Length</style></keyword><keyword><style  face="normal" font="default" size="100%">Seasonality</style></keyword><keyword><style  face="normal" font="default" size="100%">Seasons</style></keyword><keyword><style  face="normal" font="default" size="100%">Trees</style></keyword><keyword><style  face="normal" font="default" size="100%">Trees: microbiology</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year></dates><publisher><style face="normal" font="default" size="100%">Blackwell Publishing Ltd</style></publisher><volume><style face="normal" font="default" size="100%">14</style></volume><pages><style face="normal" font="default" size="100%">565-575</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">We explored the changes in richness, diversity and evenness of epiphytic (on the leaf surface) and endophytic (within leaf tissues) bacteria and fungi in the foliar phyllosphere of Quercus ilex, the dominant tree species of Mediterranean forests. Bacteria and fungi were assessed during ontogenic development of the leaves, from the wet spring to the dry summer season in control plots and in plots subjected to drought conditions mimicking those projected for future decades. Our aim was to monitor succession in microbiota during the colonisation of plant leaves and its response to climate change. Ontogeny and seasonality exerted a strong influence on richness and diversity of the microbial phyllosphere community, which decreased in summer in the whole leaf and increased in summer in the epiphytic phyllosphere. Drought precluded the decrease in whole leaf phyllosphere diversity and increased the rise in the epiphytic phyllosphere. Both whole leaf bacterial and fungal richness decreased with the decrease in physiological activity and productivity of the summer season in control trees. As expected, the richness of epiphytic bacteria and fungi increased in summer after increasing time of colonisation. Under summer dry conditions, there was a positive relationship between TRF (terminal restriction fragments) richness and drought, both for whole leaf and epiphytic phyllosphere, and especially for fungal communities. These results demonstrate that changes in climate are likely to significantly alter microbial abundance and composition of the phyllosphere. Given the diverse functions and large number of phyllospheric microbes, the potential functional implications of such community shifts warrant exploration.</style></abstract><accession-num><style face="normal" font="default" size="100%">22289059</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Asensio, D</style></author><author><style face="normal" font="default" size="100%">Penuelas, J</style></author><author><style face="normal" font="default" size="100%">Ogaya, R</style></author><author><style face="normal" font="default" size="100%">Llusia, J</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Seasonal soil VOC exchange rates in a Mediterranean holm oak forest and their responses to drought conditions</style></title><secondary-title><style face="normal" font="default" size="100%">Atmospheric Environment</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">climate change</style></keyword><keyword><style  face="normal" font="default" size="100%">Drought</style></keyword><keyword><style  face="normal" font="default" size="100%">Mediterranean holm oak forest</style></keyword><keyword><style  face="normal" font="default" size="100%">Monoterpenes</style></keyword><keyword><style  face="normal" font="default" size="100%">Soil CO2 flux</style></keyword><keyword><style  face="normal" font="default" size="100%">Soil VOC exchange</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2007</style></year></dates><volume><style face="normal" font="default" size="100%">41</style></volume><pages><style face="normal" font="default" size="100%">2456-2466</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Available information on soil volatile organic compound (VOC) exchange, emissions and uptake, is very scarce. We here describe the amounts and seasonality of soil VOC exchange during a year in a natural Mediterranean holm oak forest growing in Southern Catalonia. We investigated changes in soil VOC dynamics in drought conditions by decreasing the soil moisture to 30% of ambient conditions by artiﬁcially excluding rainfall and water runoff, and predicted the response of VOC exchange to the drought forecasted in the Mediterranean region for the next decades by GCM and ecophysiological models. The annual average of the total (detected) soil VOC and total monoterpene exchange rates were 3.273.2 and 0.470.3 mg m 2 h 1 , respectively, in control plots. These values represent 0.003% of the total C emitted by soil at the study site as CO2 whereas the annual mean of soil monoterpene exchange represents 0.0004% of total C. Total soil VOC exchange rates in control plots showed seasonal variations following changes in soil moisture and phenology. Maximum values were found in spring (1778 mg m 2 h 1 ). Although there was no signiﬁcant global effect of drought treatment on the total soil VOC exchange rates, annual average of total VOC exchange rates in drought plots resulted in an uptake rate (0.571.8 mg m 2 h 1 ) instead of positive net emission rates. Larger soil VOC and monoterpene exchanges were measured in drought plots than in control plots in summer, which might be mostly attributable to autotrophic (roots) metabolism. The results show that the diversity and magnitude of monoterpene and VOC soil emissions are low compared with plant emissions, that they are driven by soil moisture, that they represent a very small part of the soil-released carbon and that they may be strongly reduced or even reversed into net uptakes by the predicted decreases of soil water availability in the next decades. In all cases, it seems that VOC ﬂuxes in soil might have greater impact on soil ecology than on atmospheric chemistry</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Llusia, J</style></author><author><style face="normal" font="default" size="100%">Penuelas, J</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Changes in terpene content and emission in potted Mediterranean woody plants under severe drought</style></title><secondary-title><style face="normal" font="default" size="100%">Canadian Journal of Botany</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Drought</style></keyword><keyword><style  face="normal" font="default" size="100%">Mediterranean conditions</style></keyword><keyword><style  face="normal" font="default" size="100%">terpene concentration</style></keyword><keyword><style  face="normal" font="default" size="100%">Terpene emission</style></keyword><keyword><style  face="normal" font="default" size="100%">Woody plants</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1998</style></year></dates><publisher><style face="normal" font="default" size="100%">NRC Research Press</style></publisher><volume><style face="normal" font="default" size="100%">76</style></volume><pages><style face="normal" font="default" size="100%">1366-1373</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Terpene concentration and emission were studied in potted plants of some of the most common Mediterranean woody species (Pinus halepensis L., Pistacia lentiscus L., Cistus albidus L., Cistus monspeliensis L., Quercus ilex L., Quercus coccifera L., Phillyrea latifolia L., Phillyrea angustifolia L., and Arbutus unedo L.) under irrigation and under severe drought conditions that dropped relative water content to a range between 40% in Q. ilex and 85% in Phillyrea latifolia after withholding watering for one dry summer week. Terpene concentrations were detected in Pinus halepensis, Pistacia lentiscus, C. albidus, and C. monspeliensis, and they increased after withholding watering except in C. albidus. Terpene emission was detected in all species except Phillyrea angustifolia and A. unedo. Pinus halepensis showed the highest diurnal terpene emission rates of 86 µg·g-1 dry wt.·h-1 followed by C. albidus, Pistacia lentiscus, Q. ilex, Q. coccifera, Phillyrea latifolia, and C. monspeliensis (4 µg·g-1 dry wt.·h-1). Emitted terpenes represented from 0.33% of C fixed in C. monspeliensis to 10% in C. albidus. All species severely decreased their terpene emission rates under severe drought conditions. Emission by terpene-storing species (e.g., Pinus halepensis) was more related to temperature than in nonstoring species (e.g., Q. ilex), which showed emission relationships with photosynthetic rates. The monoterpenes alpha-pinene, beta-pinene, beta-phellandrene, and limonene and the sesquiterpene caryophyllene were the most abundant terpenes stored and emitted by these Mediterranean plant species.Key words: drought, Mediterranean conditions, terpene concentration, terpene emission, woody plants</style></abstract><notes><style face="normal" font="default" size="100%">doi: 10.1139/b98-141</style></notes><research-notes><style face="normal" font="default" size="100%">doi: 10.1139/b98-141</style></research-notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Filella, I</style></author><author><style face="normal" font="default" size="100%">Llusia, J</style></author><author><style face="normal" font="default" size="100%">Piñol, J</style></author><author><style face="normal" font="default" size="100%">Penuelas, J</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Leaf gas exchange and ﬂuorescence of Phillyrea latifolia, Pistacia lentiscus and Quercus ilex saplings in severe drought and high temperature conditions</style></title><secondary-title><style face="normal" font="default" size="100%">Environmental and Experimental Botany</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Drought</style></keyword><keyword><style  face="normal" font="default" size="100%">hF/F’m</style></keyword><keyword><style  face="normal" font="default" size="100%">phillyrea latifolia</style></keyword><keyword><style  face="normal" font="default" size="100%">photosynthetic rate</style></keyword><keyword><style  face="normal" font="default" size="100%">Pistacia lentiscus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">Stomatal conductance</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1998</style></year></dates><volume><style face="normal" font="default" size="100%">39</style></volume><pages><style face="normal" font="default" size="100%">213-220</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Saplings of Phillyrea latifolia, Pistacia lentiscus and Quercus ilex were witheld watering for 7 days, followed by reirrigation. Incident photosynthetic photon ﬂux density (PPFD), leaf temperature, net photosynthetic rates, stomatal conductance, and photochemical efﬁciency of the photosystem II (DF:F’m) were measured three times during the day. The watered plants had higher photosynthetic rates, stomatal conductances, DF:F’m and ETR than non-watered plants. However, watered plants were mildly water stressed as shown by low ratio of variable to maximal ﬂuorescence (Fv:Fm) and high non-photochemical ﬂuorescence quenching (qN). Their DF:F%m was low in the morning and increased in the evening, following the variations in PPFD. Watered plants of Q. ilex had lower photosynthetic activity, stomatal conductance and photosynthetic radiation use efﬁciency than Ph. latifolia and P. lentiscus, and, conversely, reached the highest DF:F%m and ETR. This seems to indicate a different relationship between photosynthetic activity and electron transport rate in Q. ilex compared to the other two species. Ph. latifolia and P. lentiscus appeared to be better adapted to severe drought than Q. ilex.</style></abstract></record></records></xml>