<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Rico, L</style></author><author><style face="normal" font="default" size="100%">Ogaya, R</style></author><author><style face="normal" font="default" size="100%">Terradas, J</style></author><author><style face="normal" font="default" size="100%">Penuelas, J</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Community structures of N-2-fixing bacteria associated with the phyllosphere of a Holm oak forest and their response to drought</style></title><secondary-title><style face="normal" font="default" size="100%">PLANT BIOLOGY</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Bacterial TRF richness</style></keyword><keyword><style  face="normal" font="default" size="100%">diazotrophic community</style></keyword><keyword><style  face="normal" font="default" size="100%">Drought</style></keyword><keyword><style  face="normal" font="default" size="100%">foliar phyllosphere</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">t-RFLP</style></keyword><keyword><style  face="normal" font="default" size="100%">Trees</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year></dates><publisher><style face="normal" font="default" size="100%">WILEY-BLACKWELL</style></publisher><pub-location><style face="normal" font="default" size="100%">111 RIVER ST, HOBOKEN 07030-5774, NJ USA</style></pub-location><volume><style face="normal" font="default" size="100%">16</style></volume><pages><style face="normal" font="default" size="100%">586-593</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Biological nitrogen (N) fixation is a key pathway in terrestrial ecosystems and is therefore critical for understanding the responses of ecosystems to global environmental changes. The free-living diazotrophic community is distributed along the canopy-to-soil profile, but the ecological significance of epiphyllic N-2 fixers, despite their functional relevance, on plant foliar surfaces remains very poorly understood compared with the N-2-fixing community in forest litter and soils. We assessed the community structure of N-2 fixers and overall bacteria by genetic fingerprinting (t-RFLP) to explore the seasonal successional patterns of the microbial community in the natural phyllosphere of a Holm oak (Quercus ilex) forest submitted to 12-year field experiment of rain exclusion mimicking the conditions of drought projected for the coming decades. Leaves of Holm oak were analysed in different seasons over a period of 1.5years. The bacterial community of the phyllosphere did not correspond to the surrounding soil biome in the same area. These analyses provided field evidence for the presence of free-living diazotrophs associated with the tissues of leaves of Holm oak, the dominant tree species of many Mediterranean forests. The results also revealed that the community composition is affected seasonally and inter-annually by the environment, and that the composition shifts in response to climate change. Drought treatment increased the richness of the epiphyllic microbial community, especially during the summer. These changes were associated with higher C:N ratios of leaves observed in response to drought in semiarid areas. This epiphyllic microbiota that can potentially fix N-2 extends the capacity of plants to adapt to the environment.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Llusia, J</style></author><author><style face="normal" font="default" size="100%">Penuelas, J</style></author><author><style face="normal" font="default" size="100%">Alessio, G a.</style></author><author><style face="normal" font="default" size="100%">Ogaya, R</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Species-specific, seasonal, inter-annual, and historically-accumulated changes in foliar terpene emission rates in Phillyrea latifolia and Quercus ilex submitted to rain exclusion in the Prades Mountains (Catalonia)</style></title><secondary-title><style face="normal" font="default" size="100%">Russian Journal of Plant Physiology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">isoprenoid emission</style></keyword><keyword><style  face="normal" font="default" size="100%">Monoterpenes</style></keyword><keyword><style  face="normal" font="default" size="100%">phillyrea latifolia</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">water stress</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year></dates><volume><style face="normal" font="default" size="100%">58</style></volume><pages><style face="normal" font="default" size="100%">126-132</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Mediterranean vegetation emits large amounts of terpenes. We aimed to study the effects of the decreases in soil water availability forecast for the next decades by global circulation models and ecophysio logical models on the terpene emissions by two widely distributed Mediterranean woody species, Phillyrea latifolia L. and Quercus ilex L. We subjected holm oak forest plots to an experimental soil drought of ca. 20% decrease in soil moisture by partial rainfall exclusion and runoff exclusion. We measured the emission rates throughout the seasons for two years with contrasting precipitation and soil moisture (16.6% average in 2003 vs. 6.4% as average in 2005). Among the detected volatile terpenes, only αpinene and limonene were present in detectable quantities in all of the studied periods. Total terpene emitted ranged from practically zero (spring 2003) to 3.6 and 58.3 μg/(g dry wt h) (winter 2005 and summer 2003 for P. latifolia and Q. ilex, respec tively). A clear seasonality was found in the emission rates (they were the highest in summer in both species) and also in the qualitative composition of the emission mix. Maximum emissions of αpinene occurred in spring and maximum emissions of limonene in winter. Neither the interannual differences in water availabil ity nor the rain exclusion treatment significantly affected the emissions in P. latifolia, but Q. ilex showed by 17% lower emissions during the drier second year of study, 2005, but more than two and threefold increases with the drought treatment in summer 2003 and in summer 2005, respectively, showing historical accumu lated effects. These results, which show increased monoterpene emission under the moderate drought pro duced by the treatment and decreased emission under the severe second year drought, and a much higher sen sitivity to drought in Q. ilex than in P. latifolia, are useful in understanding the behavior of plant volatiles under Mediterranean conditions and in modeling future emission under changing climate conditions. They show that the usage of current models could lead to under and overestimations of the emission under summer dry conditions, because most current algorithms are based on light and temperature only.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Alessio, G A</style></author><author><style face="normal" font="default" size="100%">Penuelas, J</style></author><author><style face="normal" font="default" size="100%">De Lillis, M</style></author><author><style face="normal" font="default" size="100%">Llusia, J</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Implications of foliar terpene content and hydration on leaf flammability of Quercus ilex and Pinus halepensis</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Biology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">fire</style></keyword><keyword><style  face="normal" font="default" size="100%">foliar hydration</style></keyword><keyword><style  face="normal" font="default" size="100%">leaf flammability</style></keyword><keyword><style  face="normal" font="default" size="100%">Pinus halepensis</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">terpene content</style></keyword><keyword><style  face="normal" font="default" size="100%">water content</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2008</style></year></dates><publisher><style face="normal" font="default" size="100%">Blackwell Publishing Ltd</style></publisher><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">123-128</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">We investigated the implications of foliar hydration and terpene content on leaf flammability in two widely distributed forest species of the Mediterranean basin, Quercus ilex, which does not store terpenes, and Pinus halepensis, a terpene-storing species. The experiments were carried out in plants grown under different water regimes that generated a wide range of foliar hydration and terpene contents. We monitored the temperatures and time elapsed to reach the smoke, pyrolysis and flame phases. Smoke appeared much earlier (37 versus 101 s) and at lower temperatures (96 versus 139 °C) in Quercus ilex than in Pinus halepensis. Quercus ilex reached pyrolysis earlier than Pinus halepensis (278 versus 338 s) but at the same temperature (365–371 °C). There were no significant differences in time elapsed nor in temperature for flammability (386–422 s; 505–487 °C in both species). Quercus ilex had lower water hydration than Pinus halepensis (41 versus 100%) and the leaf content of terpenes in Quercus was three orders of magnitude lower. The results of this study show no differences in the flame phase between the two species and the absence of a significant relationship between temperature and elapsed time of the different flammability phases in relation to monoterpene content; thus indicating that the role of monoterpenes in flammability phases is smaller than that of the water content. This, however, does not exclude the effects of terpene content on plant combustibility and fire propagation once fires start.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Alessio, G A</style></author><author><style face="normal" font="default" size="100%">Penuelas, J</style></author><author><style face="normal" font="default" size="100%">Llusia, J</style></author><author><style face="normal" font="default" size="100%">Ogaya, R</style></author><author><style face="normal" font="default" size="100%">Estiarte, M</style></author><author><style face="normal" font="default" size="100%">De Lillis, M</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Influence of water and terpenes on flammability in some dominant Mediterranean species</style></title><secondary-title><style face="normal" font="default" size="100%">INTERNATIONAL JOURNAL OF WILDLAND FIRE</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Arbutus unedo</style></keyword><keyword><style  face="normal" font="default" size="100%">Cistus albidus</style></keyword><keyword><style  face="normal" font="default" size="100%">emission and content of volatile compounds</style></keyword><keyword><style  face="normal" font="default" size="100%">Erica multiflora</style></keyword><keyword><style  face="normal" font="default" size="100%">Globularia alypum</style></keyword><keyword><style  face="normal" font="default" size="100%">phillyrea latifolia</style></keyword><keyword><style  face="normal" font="default" size="100%">Pinus halepensis</style></keyword><keyword><style  face="normal" font="default" size="100%">Pistacia lentiscus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">Rosmarinus officinalis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2008</style></year></dates><publisher><style face="normal" font="default" size="100%">CSIRO PUBLISHING</style></publisher><pub-location><style face="normal" font="default" size="100%">150 OXFORD ST, PO BOX 1139, COLLINGWOOD, VICTORIA 3066, AUSTRALIA</style></pub-location><volume><style face="normal" font="default" size="100%">17</style></volume><pages><style face="normal" font="default" size="100%">274-286</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">In the Mediterranean basin, fires are a major concern for forest and shrubland ecosystems. We studied flammability, its seasonality and its relationship with leaf moisture and volatile terpene content and emission in the dominant species of a Mediterranean shrubland and forest in Catalonia ( NE Iberian Peninsula). We measured temperatures and time elapsed between the three flammability phases: smoke, pyrolysis and flame, for four seasons. We sampled twice in spring because of an occasional drought period during this season. Flammability had a significant relationship with leaf hydration, in the shrubland and in the forest. Few and only weak correlations were found between terpene content and flammability. In the future, arid conditions projected by climatic and ecophysiological models will increase fire risk through decreased hydration and subsequent increased flammability of the species.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Serrano, L</style></author><author><style face="normal" font="default" size="100%">Penuelas, J</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Contribution of physiological and morphological adjustments to drought resistance in two Mediterranean tree species</style></title><secondary-title><style face="normal" font="default" size="100%">Biologia Plantarum</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Drought stress</style></keyword><keyword><style  face="normal" font="default" size="100%">net photosynthetic rate</style></keyword><keyword><style  face="normal" font="default" size="100%">phillyrea latifolia</style></keyword><keyword><style  face="normal" font="default" size="100%">plant and tissue-water relations</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">Stomatal conductance</style></keyword><keyword><style  face="normal" font="default" size="100%">water potential</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2005</style></year></dates><volume><style face="normal" font="default" size="100%">49</style></volume><pages><style face="normal" font="default" size="100%">551-559</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Plant water potential (ψ), its components, and gas exchange data of two Mediterranean co-occurring woody species (Quercus ilex L. and Phillyrea latifolia L.) were measured in response to seasonal changes in water availability over two consecutive years. The relative contribution of physiological and morphological adjustments to drought resistance was assessed through Principal Component Analyses. There were large adjustments in stomatal conductance (~36 % of accounted variance). Net photosynthetic rate and water use efficiency were closely tuned to water availability and accounted for ~17 % of variance. The slope of the water potential vs. relative water content (dψ/dRWC0) below zero pressure potential increased as a result of seasonal and ontogenic increases in apoplastic water fraction and accounted for ~20 % variance. This tolerance mechanism was accompanied by an increased range of positive pressure potential, suggesting a functional role of sclerophylly in these Mediterranean evergreens. Similarly, changes in the slope of dψ/dRWC in the range of positive pressure potential (~13 % of accounted variance) were associated to variations in cell wall elasticity and resulted in lower RWC at zero pressure potential. When considering the species studied separately, the results indicated the primary role of stomatal regulation in the drought resistance of Q. ilex, while increased apoplastic water fraction had a major contribution in the drought resistance of P. latifolia.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Penuelas, J</style></author><author><style face="normal" font="default" size="100%">Llusia, J</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Short-term responses of terpene emission rates to experimental changes of PFD in Pinus halepensis and Quercus ilex in summer field conditions</style></title><secondary-title><style face="normal" font="default" size="100%">Environmental and Experimental Botany</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">a-pinene</style></keyword><keyword><style  face="normal" font="default" size="100%">d 3 -carene</style></keyword><keyword><style  face="normal" font="default" size="100%">field conditions</style></keyword><keyword><style  face="normal" font="default" size="100%">limonene</style></keyword><keyword><style  face="normal" font="default" size="100%">pfd</style></keyword><keyword><style  face="normal" font="default" size="100%">photosynthetic rate</style></keyword><keyword><style  face="normal" font="default" size="100%">Pinus halepensis</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">Temperature</style></keyword><keyword><style  face="normal" font="default" size="100%">Terpene emission</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1999</style></year></dates><volume><style face="normal" font="default" size="100%">42</style></volume><pages><style face="normal" font="default" size="100%">61-68</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The diurnal relationships of monoterpene emission with PFD and photosynthetic rates were studied in the Mediterranean trees Pinus halepensis, a terpene storing species, and Quercus ilex, a non-storing species, under summer ﬁeld conditions. At morning, midday and evening, leaves were submitted to different irradiance levels by differentially shading them. Both species emitted large amounts of monoterpenes (about 20 mg g DM1 h1 ). The most emitted terpene by P. halepensis was D 3 -carene followed by b-myrcene, a-pinene and b-pinene. The most emitted terpene by Q. ilex was limonene followed by a-pinene and b-pinene. No clear correlation to temperature was found for Q. ilex emission within the diurnal range of 21–33°C, whereas P. halepensis emissions increased with the temperature. There was no consistent link between terpene emissions and PFD or photosynthetic rates for P. halepensis but there were signiﬁcant relationships for Q. ilex. However, emissions by Q. ilex became inhibited at highest PFDs and during the course of the day when net photosynthesis decreased under summer drought. The study shows that the emission rates of both species are highly variable and that during a hot Mediterranean summer day (with limited water availability) the diurnal variation of emission is not only driven by the common light and temperature dependencies. Water stress, branch-to-branch variability and other inﬂuences must explain great part of the observed variability</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Penuelas, J</style></author><author><style face="normal" font="default" size="100%">Filella, I</style></author><author><style face="normal" font="default" size="100%">Llusia, J</style></author><author><style face="normal" font="default" size="100%">Siscart, D</style></author><author><style face="normal" font="default" size="100%">Piñol, J</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Comparative field study of spring and summer leaf gas exchange and photobiology of the mediterranean trees Quercus ilex and Phillyrea latifolia</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Experimental Botany</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">fluorescence</style></keyword><keyword><style  face="normal" font="default" size="100%">intrinsic water use efficiency</style></keyword><keyword><style  face="normal" font="default" size="100%">net photosynthetic rates</style></keyword><keyword><style  face="normal" font="default" size="100%">phillyrea latifolia</style></keyword><keyword><style  face="normal" font="default" size="100%">photochemical efficiency</style></keyword><keyword><style  face="normal" font="default" size="100%">photochemical reflectance index</style></keyword><keyword><style  face="normal" font="default" size="100%">photosynthetic radiation-use-efficiency</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">reflectance</style></keyword><keyword><style  face="normal" font="default" size="100%">Stomatal conductance</style></keyword><keyword><style  face="normal" font="default" size="100%">summer</style></keyword><keyword><style  face="normal" font="default" size="100%">water index</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1998</style></year></dates><volume><style face="normal" font="default" size="100%">49</style></volume><pages><style face="normal" font="default" size="100%">229-238</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Summer-induced changes in gas exchange, fluorescence and reflectance were measured on leaves of two co-occurring Mediterranean small trees, Quercus ilex and Phillyrea latifolia, in May, June and July 1996 in Central Catalonia (NE Spain). The humid 1996 summer only produced mild water stress conditions. However, photosynthesis (A) and stomatal conductance (gs) decreased in June and July in both species. In June P. latifolia had higher net photosynthetic rates and lower stomatal conductances than Q. ilex, thus exhibiting higher instantaneous plant water use efficiencies. In agreement with these results, the photo-chemical reflectance index (PRI, calculated as (R570-R531)/(R531+R570)) of P. latifolia was lower, suggesting a possible lower xanthophyll de-epoxidation state. However, P. latifolia had lower ΔF/F′ and therefore a lower electron transport rate (ETR). The behaviour of PRI confirmed previous studies indicating a strong relationship between PRI, ΔF/F′ , and photosynthetic radiation-use efficiency (PRUE). PRI offers a simple, portable means of assessing PRUE with the potential for remote sensing applications. Finally, the possible ecological consequences of these results on the behaviour of the two species studied under the predicted warmer and drier conditions of global change are discussed.</style></abstract><notes><style face="normal" font="default" size="100%">10.1093/jxb/49.319.229</style></notes><research-notes><style face="normal" font="default" size="100%">10.1093/jxb/49.319.229</style></research-notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Filella, I</style></author><author><style face="normal" font="default" size="100%">Llusia, J</style></author><author><style face="normal" font="default" size="100%">Piñol, J</style></author><author><style face="normal" font="default" size="100%">Penuelas, J</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Leaf gas exchange and ﬂuorescence of Phillyrea latifolia, Pistacia lentiscus and Quercus ilex saplings in severe drought and high temperature conditions</style></title><secondary-title><style face="normal" font="default" size="100%">Environmental and Experimental Botany</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Drought</style></keyword><keyword><style  face="normal" font="default" size="100%">hF/F’m</style></keyword><keyword><style  face="normal" font="default" size="100%">phillyrea latifolia</style></keyword><keyword><style  face="normal" font="default" size="100%">photosynthetic rate</style></keyword><keyword><style  face="normal" font="default" size="100%">Pistacia lentiscus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">Stomatal conductance</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1998</style></year></dates><volume><style face="normal" font="default" size="100%">39</style></volume><pages><style face="normal" font="default" size="100%">213-220</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Saplings of Phillyrea latifolia, Pistacia lentiscus and Quercus ilex were witheld watering for 7 days, followed by reirrigation. Incident photosynthetic photon ﬂux density (PPFD), leaf temperature, net photosynthetic rates, stomatal conductance, and photochemical efﬁciency of the photosystem II (DF:F’m) were measured three times during the day. The watered plants had higher photosynthetic rates, stomatal conductances, DF:F’m and ETR than non-watered plants. However, watered plants were mildly water stressed as shown by low ratio of variable to maximal ﬂuorescence (Fv:Fm) and high non-photochemical ﬂuorescence quenching (qN). Their DF:F%m was low in the morning and increased in the evening, following the variations in PPFD. Watered plants of Q. ilex had lower photosynthetic activity, stomatal conductance and photosynthetic radiation use efﬁciency than Ph. latifolia and P. lentiscus, and, conversely, reached the highest DF:F%m and ETR. This seems to indicate a different relationship between photosynthetic activity and electron transport rate in Q. ilex compared to the other two species. Ph. latifolia and P. lentiscus appeared to be better adapted to severe drought than Q. ilex.</style></abstract></record></records></xml>