<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>3</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">CELESTINO, C</style></author><author><style face="normal" font="default" size="100%">HERNÁNDEZ, I</style></author><author><style face="normal" font="default" size="100%">Lopez-Vela, D</style></author><author><style face="normal" font="default" size="100%">CARNEROS, E</style></author><author><style face="normal" font="default" size="100%">ALEGRE, J</style></author><author><style face="normal" font="default" size="100%">TORIBIO, M</style></author><author><style face="normal" font="default" size="100%">Fernández-Guijarro, B</style></author><author><style face="normal" font="default" size="100%">Cardo, L</style></author></authors><secondary-authors><author><style face="normal" font="default" size="100%">Santamaria, JM and Desjardins, Y</style></author></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">First data from a field trial of Quercus suber plants regenerated from mature selected trees and from their half-sib progenies by somatic embryogenesis</style></title><secondary-title><style face="normal" font="default" size="100%">Proceedings of the Second International Symposium on Acclimatization and Establishment of Micropropagated Plants</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">field establishment</style></keyword><keyword><style  face="normal" font="default" size="100%">rejuvenation</style></keyword><keyword><style  face="normal" font="default" size="100%">Somatic embryogenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">woody plant micro-propagation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2007</style></year></dates><publisher><style face="normal" font="default" size="100%">INTERNATIONAL SOCIETY HORTICULTURAL SCIENCE</style></publisher><pub-location><style face="normal" font="default" size="100%">PO BOX 500, 3001 LEUVEN 1, BELGIUM</style></pub-location><pages><style face="normal" font="default" size="100%">215-218</style></pages><isbn><style face="normal" font="default" size="100%">978-90-6605-550-6</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Somatic embryogenesis is considered as the most suitable current regeneration technique for the conservation and genetic improvement of tree species. The ability to induce somatic embryogenesis in leaves from mature trees gives the possibility to compare the performance of the parent trees and their progenies at the same age and place of test. Somatic seedlings were regenerated from five selected cork oak trees and from young plants of their half-sib progenies. They were planted in the field conjointly with zygotic seedlings of these families, to test the comparisons somatic vs. zygotic origin and somatic from mature trees vs. somatic from juvenile seedlings. The effect of genotype and kind of progeny will be studied in a complete factorial design; therefore the field trial comprised 15 treatments arranged in five completely randomised blocks. Each experimental unit included three plants that were distributed following a row and column pattern. Fist survival and height data after six and twelve months of establishment are provided. All plants from seeds survived while almost the half of somatic seedlings died after the winter and they were replaced. Almost 70% of these plants survived after the following summer. There were no differences between somatic seedlings of mature or juvenile origin. Zygotic seedlings doubled the height of the somatic ones after one year in the field, and within these plants those of mature origin grew slightly more than those of juvenile source. Differences due to genotype and family effects were noticeable. No apparent morphological alterations were detected among the three kinds of tested progenies.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Álvarez, R</style></author><author><style face="normal" font="default" size="100%">Alonso, P</style></author><author><style face="normal" font="default" size="100%">Cortizo, M</style></author><author><style face="normal" font="default" size="100%">CELESTINO, C</style></author><author><style face="normal" font="default" size="100%">HERNÁNDEZ, I</style></author><author><style face="normal" font="default" size="100%">TORIBIO, M</style></author><author><style face="normal" font="default" size="100%">Ordás, R J</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Genetic transformation of selected mature cork oak (Quercus suber L.) trees.</style></title><secondary-title><style face="normal" font="default" size="100%">Plant cell reports</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Agrobacterium</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Fagaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">Somatic embryogenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Tree genetic transformation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2004</style></year></dates><volume><style face="normal" font="default" size="100%">23</style></volume><pages><style face="normal" font="default" size="100%">218-223</style></pages><isbn><style face="normal" font="default" size="100%">3498510479</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">A transformation system for selected mature cork oak (Quercus suber L.) trees using Agrobacterium tumefaciens has been established. Embryos obtained from recurrent proliferating embryogenic masses were inoculated with A. tumefaciens strains EHA105, LBA4404 or AGL1 harbouring the plasmid pBINUbiGUSint [carrying the neomycin phosphotransferase II (nptII) and beta-glucuronidase (uidA) genes]. The highest transformation efficiency (4%) was obtained when freshly isolated explants were inoculated with A. tumefaciens strain AGL1. Evidence of stable transgene integration was obtained by PCR for the nptII and uidA genes, Southern blotting and expression of the uidA gene. The transgenic embryos were germinated and successfully transferred to soil.</style></abstract><accession-num><style face="normal" font="default" size="100%">15185122</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">HERNÁNDEZ, I</style></author><author><style face="normal" font="default" size="100%">CELESTINO, C</style></author><author><style face="normal" font="default" size="100%">TORIBIO, M</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Vegetative propagation of Quercus suber L. by somatic embryogenesis. I. Factors affecting the induction in leaves from mature cork oak trees.</style></title><secondary-title><style face="normal" font="default" size="100%">Plant cell reports</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Clonal propagation</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">Somatic embryogenesis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2003</style></year></dates><volume><style face="normal" font="default" size="100%">21</style></volume><pages><style face="normal" font="default" size="100%">759-764</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Somatic embryogenesis was induced in expanding leaves from epicormic shoots forced to sprout from segments of branches collected from several hundred-year-old cork oak trees. Following a basic protocol previously defined for leaves taken from seedlings of this species, several factors were studied to improve the response. The induction frequency was significantly higher when the length of exposure to growth regulators was increased from 7 to 30 days. The combined application of NAA and BAP was essential for induction. Although both regulators had a very significant influence, their interaction was not significant, suggesting independent roles. Leaf size had a crucial effect, because beyond a certain threshold, embryogenesis could not be obtained. Embryogenic lines were maintained via repetitive embryogenesis on hormone-free medium for more than 2 years.</style></abstract><accession-num><style face="normal" font="default" size="100%">12789519</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">HERNÁNDEZ, I</style></author><author><style face="normal" font="default" size="100%">CELESTINO, C</style></author><author><style face="normal" font="default" size="100%">ALEGRE, J</style></author><author><style face="normal" font="default" size="100%">TORIBIO, M</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Vegetative propagation of Quercus suber L. by somatic embryogenesis. II. Plant regeneration from selected cork oak trees.</style></title><secondary-title><style face="normal" font="default" size="100%">Plant cell reports</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Clonal propagation</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">Selected trees</style></keyword><keyword><style  face="normal" font="default" size="100%">Somatic embryogenesis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2003</style></year></dates><volume><style face="normal" font="default" size="100%">21</style></volume><pages><style face="normal" font="default" size="100%">765-770</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The regeneration of somatic seedlings from selected 100-year-old cork oak trees is reported. The induction of somatic embryogenesis from leaves of epicormic shoots was significantly affected by genotype, harvesting time and their interaction. Leaves from all five selected trees produced somatic embryos when the segments of branches used as sources of epicormic shoots were collected in May. Genotype, but not the level of photosynthetically active radiation, affected the proliferation of the embryogenic lines and the number of detachable embryos that could be obtained from them. Genotype also affected several steps leading to conversion of somatic embryos, from germination to complete acclimatisation of somatic seedlings. Almost 40% of the somatic embryos from all lines germinated, showing coordinated root and shoot growth. Although the mean percentage of recovery for the whole process was low, plants could be regenerated from four of the five trees tested.</style></abstract><accession-num><style face="normal" font="default" size="100%">12789520</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Hornero, J</style></author><author><style face="normal" font="default" size="100%">Martinez, I</style></author><author><style face="normal" font="default" size="100%">CELESTINO, C</style></author><author><style face="normal" font="default" size="100%">Gallego, F J</style></author><author><style face="normal" font="default" size="100%">Torres, V</style></author><author><style face="normal" font="default" size="100%">TORIBIO, M</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">EARLY CHECKING OF GENETIC STABILITY OF CORK OAK SOMATIC EMBRYOS BY AFLP ANALYSIS.</style></title><secondary-title><style face="normal" font="default" size="100%">International Journal of Plant Sciences</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">AFLP</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA markers</style></keyword><keyword><style  face="normal" font="default" size="100%">in vitro culture</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">somaclonal variation</style></keyword><keyword><style  face="normal" font="default" size="100%">Somatic embryogenesis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2001</style></year></dates><publisher><style face="normal" font="default" size="100%">University of Chicago Press</style></publisher><volume><style face="normal" font="default" size="100%">162</style></volume><pages><style face="normal" font="default" size="100%">827</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Early checking of the genetic stability, of tissue culture-derived plants is necessary to obtain all the potential benefits of clonal forestry. Previous work in Quercus suber L. using random amplified polymorphic DNA (RAPD) markers showed no genetic variation among somatic embryos within embryogenic lines of zygotic origin. Genetic fingerprinting based on amplified fragment length polymorphisms (AFLP) allows the direct analysis of variation at the entire DNA level by generating more reproducible markers than RAPDs. To confirm the absence of genetic variation within six embryogenic lines of zygotic origin, six primer pairs were selected out of 48 combinations of primers for revealing up to 512 AFLP markers, 301 of them (58.8%) being polymorphic. The mean number of markers per genotype was 375. Again differences were recorded among embryogenic lines, even between those that arose from half-sib zygotic embryos, but no variation was observed among somatic embryos within embryogenic lines. To check variation in embryogenic lines raised from non-embryonic tissues, somatic embryogenesis was induced in expanding leaves collected from sprouts originating in three mature cork oak. DNA was extracted from leaves and from somatic embryos derived from each tree, and three primer pairs showed 165, 110, and 108 markers per genotype/tree, respectively. In one tree, AFLP patterns generated from leaves and somatic embryos were identical, but variation was detected in samples from the other two trees. Although the level of genetic variation detected in these lines (5.6% and 7.3% of polymorphism, respectively) is lower than that recorded for half sibs of cork oak (25%–31%), its influence on phenotypic variation needs further assessment. [ABSTRACT FROM AUTHOR]</style></abstract><notes><style face="normal" font="default" size="100%">Accession Number: 4694993; Hornero, J. Martínez, I. Celestino, C. Gallego, F.J. Torres, V. Toribio, M.; Source Info: Jul2001, Vol. 162 Issue 4, p827; Subject Term: CORK oak; Subject Term: GENETIC polymorphisms; Number of Pages: 7p; Illustrations: 3 Charts, 3 Graphs; Document Type: Article; Full Text Word Count: 4881</style></notes><research-notes><style face="normal" font="default" size="100%">Accession Number: 4694993; Hornero, J. Martínez, I. Celestino, C. Gallego, F.J. Torres, V. Toribio, M.; Source Info: Jul2001, Vol. 162 Issue 4, p827; Subject Term: CORK oak; Subject Term: GENETIC polymorphisms; Number of Pages: 7p; Illustrations: 3 Charts, 3 Graphs; Document Type: Article; Full Text Word Count: 4881</style></research-notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">TORIBIO, M</style></author><author><style face="normal" font="default" size="100%">CELESTINO, C</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cultivo in vitro del alcornoque</style></title><secondary-title><style face="normal" font="default" size="100%">Scientia gerundensis</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">cork-oak</style></keyword><keyword><style  face="normal" font="default" size="100%">micropropagation</style></keyword><keyword><style  face="normal" font="default" size="100%">plant regeneration</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber L.</style></keyword><keyword><style  face="normal" font="default" size="100%">Somatic embryogenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">tissue culture</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1989</style></year></dates><volume><style face="normal" font="default" size="100%">15</style></volume><pages><style face="normal" font="default" size="100%">11-21</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Vegetative plant propagation is mainly used in the operational line of Forest Tree Improvement Programs to capture all the genetic potential, including the non-additive component of the genetic variance. In the case of genus Quercus, it could be also used to avoid the problems derived from installation and yielding of seed orchards. The tissue culture based micropropagation arises as an alternative technique to solve the problems of space and time associated with the classical techniques of asexual propagation. This article reviews the work done with cork-oak in this field. Up to date there are few papers dealing with in vitro culture of Quercus suher L. Embryo culture has been tryed to determine the minimum requeriments of the species and its morphogenic potential. Plantlet regeneration has been obtained following axillary budding on nodal segments from juvenile and rejuvenated tissues, elongation and rooting of microcuttings. Main problems arise on transplanting soil the neoformed plants. Callus culture has been achieved from cotyledons and epicotyl segments from young seedlings. In booth cases also somatic embryogenesis has been reported. This fact opens a new way for micropropagating cork-oak, which is discussed.</style></abstract></record></records></xml>