<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Pintos, B</style></author><author><style face="normal" font="default" size="100%">Sanchez, N</style></author><author><style face="normal" font="default" size="100%">Bueno, Ma A</style></author><author><style face="normal" font="default" size="100%">Ma Navarro, R</style></author><author><style face="normal" font="default" size="100%">Jorrin, J</style></author><author><style face="normal" font="default" size="100%">Manzanera, J A</style></author><author><style face="normal" font="default" size="100%">Gomez-Garay, A</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Induction of Quercus ilex L. haploid and doubled-haploid embryos from anther cultures by temperature-stress</style></title><secondary-title><style face="normal" font="default" size="100%">SILVAE GENETICA</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">anthers</style></keyword><keyword><style  face="normal" font="default" size="100%">Holm oak</style></keyword><keyword><style  face="normal" font="default" size="100%">microsatellite DNA-markers</style></keyword><keyword><style  face="normal" font="default" size="100%">pollen embryogenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year></dates><publisher><style face="normal" font="default" size="100%">J D SAUERLANDERS VERLAG</style></publisher><pub-location><style face="normal" font="default" size="100%">BERLINER STRASSE 46, 63619 BAD ORB, GERMANY</style></pub-location><volume><style face="normal" font="default" size="100%">62</style></volume><pages><style face="normal" font="default" size="100%">210-218</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">This paper describes a method to obtain haploid and doubled-haploid (DH) embryos using anther cultures of holm oak (Quercus ilex L.). The production of haploids and DH through gametic embryogenesis provides an attractive biotechnological tool for developing homozygous lines from heterozygous parents, which is important in breeding programs, as well as in genetic studies. As a consequence, protocols to produce homozygous plants have a significant impact on forest tree improvement. Anthers were subjected to different temperature treatments for embryo induction: a cold pre-treatment (4 C) from 3 to 7 days was carried out at the beginning, followed by a heat shock (33 C) from 2 to 5 days. Most anthers responding to these stress treatments contained vacuolated microspores, indicating that this developmental stage is responsive to embryogenesis induction in holm-oak microspores. In all cases, embryos grew from the interior of the anthers, breaking through the degenerating anther walls. Under these conditions, embryo formation occurred in 31 anthers between 46 and 95 days after culture initiation. Embryo analysis performed with flow-cytometry and DNA-microsatellite markers showed haploid profiles and/or spontaneous doubling of the chromosomes during early regeneration stages. This is, to our knowledge, the first published report on gametic embryogenesis in holm oak.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>3</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">CUENCA, B</style></author><author><style face="normal" font="default" size="100%">López, C</style></author><author><style face="normal" font="default" size="100%">Pato, B</style></author><author><style face="normal" font="default" size="100%">OCAÑA, L</style></author><author><style face="normal" font="default" size="100%">Santos, M I</style></author><author><style face="normal" font="default" size="100%">Manzanera, J A</style></author><author><style face="normal" font="default" size="100%">Pintos, B</style></author><author><style face="normal" font="default" size="100%">Bueno, M A</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">CLONACIÓN DE PROGENIES DE ALCORNOQUES PLUS DE CUATRO REGIONES DE PROCEDENCIA MEDIANTE EMBRIOGÉNESIS SOMÁTICA PARA LA OBTENCIÓN DE MATERIALES FORESTALES DE REPRODUCCIÓN</style></title><secondary-title><style face="normal" font="default" size="100%">IV Congreso Forestal Español</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Corcho</style></keyword><keyword><style  face="normal" font="default" size="100%">cultivo in vitro</style></keyword><keyword><style  face="normal" font="default" size="100%">embriones</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">selección</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2005</style></year></dates><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Se ha realizado una selección fenotípica individual de árboles plus de alcornoque en los rodales selectos establecidos por el Instituto del Corcho, la Madera y el Carbón Vegetal (ICMC, Junta de Extremadura) en cuatro regiones de procedencia: Montes de Toledo-Villuercas, Sierra Morena Occidental, Sierra de San Pedro, y Norte de Cáceres-Salamanca. De los árboles seleccionados se han recogieron estaquillas en primavera de 2002 y 2003, y bellotas inmaduras durante el verano de 2002, 2003 y 2004. De las estaquillas se establecieron inicialmente 48 clones mediante cultivo de yemas axilares (MANZANERA y PARDOS, 1990). La elevada edad de los árboles hizo imposible la estabilización y mantenimiento de los cultivos y actualmente sólo sobrevive 1 de los clones. De los embriones cigóticos inmaduros, según protocolo de BUENO et al. (1992), se consiguió inducir embriogénesis somática en los 55 árboles muestreados de los 61 seleccionados. Estas líneas son regularmente subcultivadas, y los embriones obtenidos madurados, germinados y aclimatados para el establecimiento de ensayos de progenie con propósito de disponer de materiales de mejora. En enero de 2005 se estableció la primera finca de ensayo y actualmente se dispone de 1400 vitroplantas más para el establecimiento de nuevas parcelas. Palabras</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Pintos, B</style></author><author><style face="normal" font="default" size="100%">Manzanera, J A</style></author><author><style face="normal" font="default" size="100%">Bueno, M A</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cytological analysis of early microspore divisions leading to gametic embryo formation in Quercus suber L. anther cultures</style></title><secondary-title><style face="normal" font="default" size="100%">Acta Physiologiae Plantarum</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">anther culture</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">microspore embryogenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2005</style></year></dates><volume><style face="normal" font="default" size="100%">27</style></volume><pages><style face="normal" font="default" size="100%">703-708</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The cor re la tion be tween the phenologic stage of the in flo res - cence and the microspore de vel op ment stage was stud ied. Cyto log i cal ex am i na tions of the de vel op ment of micro spores dur - ing in vi tro an ther cul ture of cork oak (Quercus suber L.), were car ried out dur ing the first four weeks of cul ture. To ob serve the di vi sion oc cur ring in the micro spores, an thers were taken ran domly from the cultures after heat shock treatment and were stained with DAPI. Most of the an thers re spond ing to a heat stress treat ment con tained 91 % vacuolated micro spores, in di cat ing that this de vel op men tal stage is re spon sive to embryo genesis in duc tion in cork-oak micro spores. Af ter the heat shock treat ment some cork-oak micro spores were in duced and ini ti ated the embryogenic path way with the oc cur rence of nu mer ous sym met ric mi to sis, pro duc ing struc tures with two to ten or more nu clei. These lead to the for ma tion of high num - bers of multicellular cork-oak micro spores (pro-em bryos). Twenty-forty days af ter in duc tion, small white glob u lar and cotyledonal em bryos were ob served, which fur ther de vel oped root and shoot, re gen er at ing plantlets.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">GÓMEZ, A</style></author><author><style face="normal" font="default" size="100%">Pintos, B</style></author><author><style face="normal" font="default" size="100%">Aguiriano, E</style></author><author><style face="normal" font="default" size="100%">Manzanera, J A</style></author><author><style face="normal" font="default" size="100%">Bueno, M A</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">SSR Markers for Quercus suber Tree Identification and Embryo Analysis</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Heredity</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">haploid embryos</style></keyword><keyword><style  face="normal" font="default" size="100%">identification (PG)</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">SSR markers</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2001</style></year></dates><volume><style face="normal" font="default" size="100%">92</style></volume><pages><style face="normal" font="default" size="100%">292-295</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Three Quercus simple sequence repeat (SSR) markers were amplified by polymerase chain reaction (PCR) from nuclear DNA extracts of trees and in vitro-induced haploid embryos from anther cultures of Quercus suber L. These markers were sufficiently polymorphic to identify 10 of 12 trees located in two Spanish natural areas. The same loci have been analyzed in anther-derived haploid embryos showing the parental tree allele segregation. All the alleles were present in the haploid progeny. The presence of diverse alleles in embryos derived from the same anther demonstrated that they were induced on multiple microspores or pollen grains and they were not clonally propagated. Also, diploid cultures and mixtures of haploid-diploid tissues were obtained. The origin of such cultures, either somatic or gametic, was elucidated by SSR markers. All the embryos showed only one allele, corroborating a haploid origin. Allelic composition of the haploid progeny permitted parental identification among all analyzed trees.</style></abstract><notes><style face="normal" font="default" size="100%">10.1093/jhered/92.3.292</style></notes><research-notes><style face="normal" font="default" size="100%">10.1093/jhered/92.3.292</style></research-notes></record></records></xml>