<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Bodénès, Catherine</style></author><author><style face="normal" font="default" size="100%">Chancerel, Emilie</style></author><author><style face="normal" font="default" size="100%">Gailing, Oliver</style></author><author><style face="normal" font="default" size="100%">Vendramin, Giovanni G</style></author><author><style face="normal" font="default" size="100%">Bagnoli, Francesca</style></author><author><style face="normal" font="default" size="100%">Durand, Jerome</style></author><author><style face="normal" font="default" size="100%">Goicoechea, Pablo G</style></author><author><style face="normal" font="default" size="100%">Soliani, Carolina</style></author><author><style face="normal" font="default" size="100%">Villani, Fiorella</style></author><author><style face="normal" font="default" size="100%">Mattioni, Claudia</style></author><author><style face="normal" font="default" size="100%">Koelewijn, Hans Peter</style></author><author><style face="normal" font="default" size="100%">Murat, Florent</style></author><author><style face="normal" font="default" size="100%">Salse, Jerome</style></author><author><style face="normal" font="default" size="100%">Roussel, Guy</style></author><author><style face="normal" font="default" size="100%">Boury, Christophe</style></author><author><style face="normal" font="default" size="100%">Alberto, Florian</style></author><author><style face="normal" font="default" size="100%">Kremer, Antoine</style></author><author><style face="normal" font="default" size="100%">Plomion, Christophe</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Comparative mapping in the Fagaceae and beyond with EST-SSRs.</style></title><secondary-title><style face="normal" font="default" size="100%">BMC plant biology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">EST-SSR</style></keyword><keyword><style  face="normal" font="default" size="100%">linkage maps</style></keyword><keyword><style  face="normal" font="default" size="100%">Markers</style></keyword><keyword><style  face="normal" font="default" size="100%">oak</style></keyword><keyword><style  face="normal" font="default" size="100%">tranferability (voyant)</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">153</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Background: Genetic markers and linkage mapping are basic prerequisites for comparative genetic analyses, QTL detection and map-based cloning. A large number of mapping populations have been developed for oak, but few gene-based markers are available for constructing integrated genetic linkage maps and comparing gene order and QTL location across related species. Results: We developed a set of 573 expressed sequence tag-derived simple sequence repeats (EST-SSRs) and located 397 markers (EST-SSRs and genomic SSRs) on the 12 oak chromosomes (2n=2x=24) on the basis of Mendelian segregation patterns in 5 full-sib mapping pedigrees of two species: Quercus robur (pedunculate oak) and Quercus petraea (sessile oak). Consensus maps for the two species were constructed and aligned. They showed a high degree of macrosynteny between these two sympatric European oaks. We assessed the transferability of EST-SSRs to other Fagaceae genera and a subset of these markers was mapped in Castanea sativa, the European chestnut. Reasonably high levels of macrosynteny were observed between oak and chestnut. We also obtained diversity statistics for a subset of EST-SSRs, to support further population genetic analyses with gene-based markers. Finally, based on the orthologous relationships between the oak, Arabidopsis, grape, poplar, Medicago, and soybean genomes and the paralogous relationships between the 12 oak chromosomes, we propose an evolutionary scenario of the 12 oak chromosomes from the eudicot ancestral karyotype. Conclusions: This study provides map locations for a large set of EST-SSRs in two oak species of recognized biological importance in natural ecosystems. This first step toward the construction of a gene-based linkage map will facilitate the assignment of future genome scaffolds to pseudo-chromosomes. This study also provides an indication of the potential utility of new gene-based markers for population genetics and comparative mapping within and beyond the Fagaceae.</style></abstract><accession-num><style face="normal" font="default" size="100%">22931513</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Vernesi, Cristiano</style></author><author><style face="normal" font="default" size="100%">Rocchini, Duccio</style></author><author><style face="normal" font="default" size="100%">Pecchioli, Elena</style></author><author><style face="normal" font="default" size="100%">Neteler, Markus</style></author><author><style face="normal" font="default" size="100%">Vendramin, Giovanni G</style></author><author><style face="normal" font="default" size="100%">Paffetti, Donatella</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A landscape genetics approach reveals ecological-based differentiation in populations of holm oak (Quercus ilex L.) at the northern limit of its range</style></title><secondary-title><style face="normal" font="default" size="100%">Biological Journal of the Linnean Society</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Bayesian computation</style></keyword><keyword><style  face="normal" font="default" size="100%">Geneland</style></keyword><keyword><style  face="normal" font="default" size="100%">Italian Prealps</style></keyword><keyword><style  face="normal" font="default" size="100%">microsatellites</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year></dates><volume><style face="normal" font="default" size="100%">107</style></volume><pages><style face="normal" font="default" size="100%">458-467</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The holm oak plays a relevant role in the functioning of Mediterranean forests. In the area north of Garda Lake, Italian Prealps, holm oak populations are at the northernmost edge of their distribution. Being peripheral, these populations are of particular interest for ecological, evolutionary and conservation studies. Through an explicit individual-based landscape genetics approach, we addressed the following questions: (1) are levels of genetic variation reduced in these marginal populations compared with central populations?; (2) despite the narrow geographical scale, do individual-based analyses have some power to detect genetic differentiation?; (3) do environmental and/or climatic factors exert a role in shaping patterns of genetic variation and differentiation? Through a Bayesian method, we identified three clusters whose genetic variability can be considered to be of the same order as that recorded in central Quercus ilex populations. Although being geographically very close (&lt; 20 km), the differentiation was statistically significant (P &lt; 0.05) with global F</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Fineschi, Silvia</style></author><author><style face="normal" font="default" size="100%">Cozzolino, Salvatore</style></author><author><style face="normal" font="default" size="100%">Migliaccio, Marianna</style></author><author><style face="normal" font="default" size="100%">Musacchio, Aldo</style></author><author><style face="normal" font="default" size="100%">Innocenti, Michela</style></author><author><style face="normal" font="default" size="100%">Vendramin, Giovanni G</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Sicily represents the Italian reservoir of chloroplast DNA diversity of Quercus ilex L. (Fagaceae)</style></title><secondary-title><style face="normal" font="default" size="100%">Ann. For. Sci.</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">genetic differentiation</style></keyword><keyword><style  face="normal" font="default" size="100%">PCR-RFLP</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">SSR</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2005</style></year></dates><volume><style face="normal" font="default" size="100%">62</style></volume><pages><style face="normal" font="default" size="100%">79-84</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Chloroplast DNA polymorphism was analysed in forty-four Italian holm oak populations. Results obtained with different markers (PCR-RFLP and SSR) were congruent, showing a clear geographic structure of genetic diversity and high value of genetic differentiation (Gst = 0.80). By combining PCR-RFLP and SSR, eight haplotypes were identified in Italy, six of them in Sicily. Most populations were fixed for one haplotype. Some populations from the extreme West Mediterranean (Morocco) and the extreme East Mediterranean areas (Crete) were interpreted as reproductively isolated populations because they had completely different haplotypes. These results strongly support the hypothesis of glacial refugia existing in southern Italy, and underline the high conservation value of natural tree populations in Sicily, in which most diversity was detected.</style></abstract></record></records></xml>