<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Simeone, Marco C</style></author><author><style face="normal" font="default" size="100%">Piredda, Roberta</style></author><author><style face="normal" font="default" size="100%">Papini, Alessio</style></author><author><style face="normal" font="default" size="100%">Vessella, Federico</style></author><author><style face="normal" font="default" size="100%">Schirone, Bartolomeo</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Application of plastid and nuclear markers to DNA barcoding of Euro-Mediterranean oaks (Quercus, Fagaceae): problems, prospects and phylogenetic implications</style></title><secondary-title><style face="normal" font="default" size="100%">Botanical Journal of the Linnean Society</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cerris</style></keyword><keyword><style  face="normal" font="default" size="100%">ecology</style></keyword><keyword><style  face="normal" font="default" size="100%">ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">phylogeny</style></keyword><keyword><style  face="normal" font="default" size="100%">regional sampling</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year></dates><pages><style face="normal" font="default" size="100%">n/a--n/a</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">DNA barcoding, a species identification system based on sequences from a short, standardized DNA region, has emerged recently as a new tool for taxonomists. We investigated the discriminatory power of a subset of highly variable proposed plant barcoding loci (matK, trnH-psbA, ITS2) in Quercus, a taxonomically complex tree genus of global importance. The research included all currently recognized species and some major variants of the Mediterranean region and Europe (32 taxa) and 17 East Asian and North American species used for comparison. Based on sequence character state, we assigned unique plastid haplotypes to 40.8% of the investigated species; ITS2 increased the resolution up to 87.8% of total taxa. Nevertheless, unsuccessful genetic distance-based discrimination questioned the potential efficiency of correct species identification for future studies. Most species appeared to be nonmonophyletic in parallel phylogenetic tests. Three subgeneric groups were outlined, with different rates of within-group variability and geographical differentiation. Members of one of these groups (corresponding to the Eurasian Group Ilex) were paraphyletic to Group Quercus from the New and Old World and the Eurasian Group Cerris. The data gathered indicate that barcoding markers may help to identify closely related species clusters and contribute to the inference of major diversification and evolutionary patterns in oaks, but the methodology per se appears to be of limited efficacy in defining species limits, unless we make a profound revision of traditional Quercus taxonomic categories. © 2013 The Linnean Society of London, Botanical Journal of the Linnean Society, 2013, 00, 000–000.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Piredda, Roberta</style></author><author><style face="normal" font="default" size="100%">Simeone, Marco C</style></author><author><style face="normal" font="default" size="100%">Attimonelli, Marcella</style></author><author><style face="normal" font="default" size="100%">BELLAROSA, Rosanna</style></author><author><style face="normal" font="default" size="100%">Schirone, Bartolomeo</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Prospects of barcoding the Italian wild dendroflora: oaks reveal severe limitations to tracking species identity.</style></title><secondary-title><style face="normal" font="default" size="100%">Molecular ecology resources</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">DNA barcoding</style></keyword><keyword><style  face="normal" font="default" size="100%">forest trees</style></keyword><keyword><style  face="normal" font="default" size="100%">Italy</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">regional sampling</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">72-83</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">DNA barcoding may be particularly important in influencing ecology, economic issues, and the fundamental crisis facing biodiversity as a standardized, species-level identification tool for taxonomy assessment. Trees play important roles in the conservation of many land ecosystems, the wood trade, and the definition of biogeographical processes; nevertheless, peculiar biological, evolutionary and taxonomical features will probably constitute an intriguing challenge to barcoders. We examined whether four marker regions (trnh-psba, rbcL, rpoc1, matK) proposed by the Consortium for the Barcode of Life (CBOL) matched species taxonomy in a preliminary tree biodiversity survey of Italian forested land. Our objective was to provide a test of future in situ applications of DNA barcodes by evaluating the efficacy of species discrimination under the criteria of uniformity of methods and natural co-occurrence of the species in the main forest ecosystems. Fifty-two species were included in a floristic study. We obtained 73% total discrimination success, with trnH-psbA as the best performing marker and oaks as the least responsive plants to the markers used. A further taxon-based study of Quercus (thirty specimens, 12 species) revealed that this genus is refractory to barcoding (0% discrimination success), a probable consequence of low variation rate at the plastid genome level, hybridization, and the incidence of biogeography. We conclude that some species-rich tree genera in small geographical regions may prove exceptionally difficult to barcode. Until more efficient markers are developed, we recommend that improved and diversified sampling (multiple locations of sympatric and co-occurring congenerics) be embraced as a timely and important goal for the precise assessment of haplotype specificity to facilitate the productive application of barcoding in practice.</style></abstract><accession-num><style face="normal" font="default" size="100%">21429102</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">BELLAROSA, Rosanna</style></author><author><style face="normal" font="default" size="100%">Simeone, Marco C</style></author><author><style face="normal" font="default" size="100%">Papini, Alessio</style></author><author><style face="normal" font="default" size="100%">Schirone, Bartolomeo</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Utility of ITS sequence data for phylogenetic reconstruction of Italian Quercus spp.</style></title><secondary-title><style face="normal" font="default" size="100%">Molecular phylogenetics and evolution</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">ITS sequences</style></keyword><keyword><style  face="normal" font="default" size="100%">phylogeny</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Secondary structures</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2005</style></year></dates><volume><style face="normal" font="default" size="100%">34</style></volume><pages><style face="normal" font="default" size="100%">355-370</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Nuclear ribosomal DNA sequences encoding the 5.8S RNA and the flanking internal transcribed spacers (ITS1 and ITS2) were used to test the phylogenetic relationships within 12 Italian Quercus taxa (Fagaceae). Hypotheses of sequence orthology are tested by detailed inspection of some basic features of oak ITS sequences (i.e., general patterns of conserved domains, thermodynamic stability and predicted conformation of the secondary structure of transcripts) that also allowed more accurate sequence alignment. Analysis of ITS variation supported three monophyletic groups, corresponding to subg. Cerris, Schlerophyllodrys (=Ilex group sensu Nixon) and Quercus, as proposed by Schwarz [Feddes Rep., Sonderbeih. D, 1-200]. A derivation of the &quot;Cerris group&quot; from the &quot;Ilex group&quot; is suggested, with Q. cerris sister to the rest of the &quot;Cerris group.&quot; Quercus pubescens was found to be sister to the rest of the &quot;Quercus group.&quot; The status of hybrispecies of Q. crenata (Q. cerrisxQ. suber) and Q. morisii (Q. ilexxQ. suber) was evaluated and discussed. Finally, the phylogenetic position of the Italian species in a broader context of the genus is presented. The utility of the ITS marker to assess the molecular systematics of oaks is therefore confirmed. The importance of Italy as a region with a high degree of diversity at the population and genetic level is discussed.</style></abstract><accession-num><style face="normal" font="default" size="100%">15619447</style></accession-num></record></records></xml>