<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Gonzalez-Benito, M Elena</style></author><author><style face="normal" font="default" size="100%">Prieto, Roberto-Moreno</style></author><author><style face="normal" font="default" size="100%">Herradon, Esther</style></author><author><style face="normal" font="default" size="100%">Martin, Carmen</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cryopreservation of Quercus suber and Quercus ilex embryonic axes: in vitro culture, desiccation and cooling factors.</style></title><secondary-title><style face="normal" font="default" size="100%">Cryo letters</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cryopreservation</style></keyword><keyword><style  face="normal" font="default" size="100%">Cryopreservation: methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Cryoprotective Agents</style></keyword><keyword><style  face="normal" font="default" size="100%">Cryoprotective Agents: pharmacology</style></keyword><keyword><style  face="normal" font="default" size="100%">Culture Techniques</style></keyword><keyword><style  face="normal" font="default" size="100%">Culture Techniques: methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Desiccation</style></keyword><keyword><style  face="normal" font="default" size="100%">Desiccation: methods</style></keyword><keyword><style  face="normal" font="default" size="100%">embryonic axes</style></keyword><keyword><style  face="normal" font="default" size="100%">germination</style></keyword><keyword><style  face="normal" font="default" size="100%">germination temperature</style></keyword><keyword><style  face="normal" font="default" size="100%">Germination: drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">liquid nitrogen</style></keyword><keyword><style  face="normal" font="default" size="100%">plantlet development</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: embryology</style></keyword><keyword><style  face="normal" font="default" size="100%">Seeds</style></keyword><keyword><style  face="normal" font="default" size="100%">Seeds: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Seeds: embryology</style></keyword><keyword><style  face="normal" font="default" size="100%">Survival Rate</style></keyword><keyword><style  face="normal" font="default" size="100%">Temperature</style></keyword><keyword><style  face="normal" font="default" size="100%">water</style></keyword><keyword><style  face="normal" font="default" size="100%">Water: analysis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2002</style></year></dates><volume><style face="normal" font="default" size="100%">23</style></volume><pages><style face="normal" font="default" size="100%">283-290</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">This study examines different factors included in the cryopreservation protocols for Quercus ilex and Q. suber embryonic axes. In vitro incubation temperature played an important role in the appropriate development of Q. ilex axes, as 15 degrees C was superior to 25 degrees C. Q. suber axes proved to be more sensitive to desiccation and cooling. Poor survival (35%) was observed when axes were included into cryovials and then in liquid nitrogen, and none when immersed in sub-cooled liquid nitrogen (-210 degrees C). Q. ilex axes showed poorly organised development in vitro (c. 50% of non-cooled axes showed shoot development). However, c. 80% survival was observed after cryopreservation (either in liquid nitrogen or sub-cooled liquid nitrogen at 0.34 g water / g dry weight), of which c. 15% showed shoot development.</style></abstract><accession-num><style face="normal" font="default" size="100%">12447487</style></accession-num></record></records></xml>