<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Hornero, J</style></author><author><style face="normal" font="default" size="100%">Gallego, F J</style></author><author><style face="normal" font="default" size="100%">Martinez, I</style></author><author><style face="normal" font="default" size="100%">TORIBIO, M</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Testing the conservation of Quercus spp. microsatellites in the cork oak, Q-suber L.</style></title><secondary-title><style face="normal" font="default" size="100%">SILVAE GENETICA</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Fagaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">microsatellites</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2001</style></year></dates><publisher><style face="normal" font="default" size="100%">J D SAUERLANDERS VERLAG</style></publisher><pub-location><style face="normal" font="default" size="100%">FINKENHOFSTRASSE 21, D-60322 FRANKFURT, GERMANY</style></pub-location><volume><style face="normal" font="default" size="100%">50</style></volume><pages><style face="normal" font="default" size="100%">162-167</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The transferability of microsatellite (SSR) loci from Quercus spp. to cork oak (Quercus suber L.) was investigated. Semi-automated analysis of fluorescently-labelled PCR fragments was used to test 24 primer sets developed for Q. myrsinifolia BLUME, Q. petraea (MAT7.) LIEB. and Q. robur L. in 41 cork oak trees from four stands covering the main area of distribution of the species in Spain. Successful cross-species events occurred for 13 loci (54%). Two of them were monomorphic and another two appeared as multilocus. High levels of genetic variability were detected both for the number of alleles, 62 (7.5 per polymorphic locus, with a maximum number of 19 in locus ssrQpZAG110) and for the expected heterozygosity (mean H-E = 0.648). These results were much higher than those previously reported by other authors using allozyme loci. The usefulness of the. SSR loci successfully amplified for studies on population genetics of cork oak is discussed.</style></abstract></record></records></xml>