<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Gonzalez-Benito, M E</style></author><author><style face="normal" font="default" size="100%">Herradon, E</style></author><author><style face="normal" font="default" size="100%">Martin, C</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">The development of a protocol for the encapsulation-desiccation and in vitro culture of embryonic axes of Quercus suber L-and Q-ilex L.</style></title><secondary-title><style face="normal" font="default" size="100%">SILVAE GENETICA</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">aliginate bead</style></keyword><keyword><style  face="normal" font="default" size="100%">Dehydration</style></keyword><keyword><style  face="normal" font="default" size="100%">in vitro culture</style></keyword><keyword><style  face="normal" font="default" size="100%">oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">recalcitrant seed</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1999</style></year></dates><publisher><style face="normal" font="default" size="100%">SAUERLANDERS VERLAG</style></publisher><pub-location><style face="normal" font="default" size="100%">FINKENHOFSTRASSE 21, W-6000 FRANKFURT, GERMANY</style></pub-location><volume><style face="normal" font="default" size="100%">48</style></volume><pages><style face="normal" font="default" size="100%">25-28</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Quercus species have seeds recalcitrant against long-term storage. Cryopreservation of embryonic axes could be a feasible way of preserving their genetic diversity. Several cryopreserva- tion protocols are based on desiccation, among them the so- called encapsulation-dehydration. However, it is previously necessary to establish an adequate in vitro culture develop- ment of desiccated axes. Embryonic axes of Q. suber and Q. ilex were aseptically excised, encapsulated in alginate beads, cultur- ed in a sucrose-rich liquid medium, desiccated for different periods in a flow bench and cultured on basal WPM medium. Moisture content of encapsulated axes dropped from 74% to 71% (fresh weigh basis) to 24.5% to 21% after 6 h desiccation, respectively for the two species. Germination decreased to 20% in both species. Germination and shoot elongation of encapsu- lated embryos (non-desiccated or desiccated for 4 h) was stud- ied for both species after culture on WPM medium supplemented with different concentrations of BAP and IBA. Medium with 0.1 mgl-1 BAP resulted in a high percentage of germina- tion and development of shoots in both species</style></abstract></record></records></xml>