<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Gonzalez-Benito, M E</style></author><author><style face="normal" font="default" size="100%">Herradon, E</style></author><author><style face="normal" font="default" size="100%">Martin, C</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">The development of a protocol for the encapsulation-desiccation and in vitro culture of embryonic axes of Quercus suber L-and Q-ilex L.</style></title><secondary-title><style face="normal" font="default" size="100%">SILVAE GENETICA</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">aliginate bead</style></keyword><keyword><style  face="normal" font="default" size="100%">Dehydration</style></keyword><keyword><style  face="normal" font="default" size="100%">in vitro culture</style></keyword><keyword><style  face="normal" font="default" size="100%">oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">recalcitrant seed</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1999</style></year></dates><publisher><style face="normal" font="default" size="100%">SAUERLANDERS VERLAG</style></publisher><pub-location><style face="normal" font="default" size="100%">FINKENHOFSTRASSE 21, W-6000 FRANKFURT, GERMANY</style></pub-location><volume><style face="normal" font="default" size="100%">48</style></volume><pages><style face="normal" font="default" size="100%">25-28</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Quercus species have seeds recalcitrant against long-term storage. Cryopreservation of embryonic axes could be a feasible way of preserving their genetic diversity. Several cryopreserva- tion protocols are based on desiccation, among them the so- called encapsulation-dehydration. However, it is previously necessary to establish an adequate in vitro culture develop- ment of desiccated axes. Embryonic axes of Q. suber and Q. ilex were aseptically excised, encapsulated in alginate beads, cultur- ed in a sucrose-rich liquid medium, desiccated for different periods in a flow bench and cultured on basal WPM medium. Moisture content of encapsulated axes dropped from 74% to 71% (fresh weigh basis) to 24.5% to 21% after 6 h desiccation, respectively for the two species. Germination decreased to 20% in both species. Germination and shoot elongation of encapsu- lated embryos (non-desiccated or desiccated for 4 h) was stud- ied for both species after culture on WPM medium supplemented with different concentrations of BAP and IBA. Medium with 0.1 mgl-1 BAP resulted in a high percentage of germina- tion and development of shoots in both species</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Martin, C</style></author><author><style face="normal" font="default" size="100%">Chevalier, Y</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Post forest-fire hydrochemical behaviour of the Rimbaud catchment (Massif des Maures, France)</style></title><secondary-title><style face="normal" font="default" size="100%">ZEITSCHRIFT FUR GEOMORPHOLOGIE</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">chemical composition</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">forest fire</style></keyword><keyword><style  face="normal" font="default" size="100%">Maquis</style></keyword><keyword><style  face="normal" font="default" size="100%">maritime pine</style></keyword><keyword><style  face="normal" font="default" size="100%">runoff</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1999</style></year></dates><publisher><style face="normal" font="default" size="100%">GEBRUDER BORNTRAEGER</style></publisher><pub-location><style face="normal" font="default" size="100%">JOHANNESSTR 3A, D-70176 STUTTGART, GERMANY</style></pub-location><volume><style face="normal" font="default" size="100%">43</style></volume><pages><style face="normal" font="default" size="100%">359-381</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">In August 1990, a forest fire devastated 84 % of the Rimbaud catchment area (1.46 km(2)). This catchment presents a substratum composed of gneiss. Its relief is sharp. Soils are skeletal. The forest fire destroyed a maquis (with treelike heather and arbustus) interspersed with cork oaks and maritime pines. The fire's effects on the chemical composition of the runoff have remained light in terms of low flows. On the other hand, mineralization has greatly increased during flood, especially at the beginning of the 1990-91 water year. The paper reports on the observations conducted during four years after the fire. Dissolved outputs and hydrochemical balances are compared with results obtained on the same catchment before the forest fire, and on the Boussicaut catchment (0.74 km(2)), which is very close by, but which was not burnt.</style></abstract><notes><style face="normal" font="default" size="100%">APS</style></notes><research-notes><style face="normal" font="default" size="100%">APS</style></research-notes></record></records></xml>