<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sebastiana, Monica Mónica</style></author><author><style face="normal" font="default" size="100%">Vieira, Bruno</style></author><author><style face="normal" font="default" size="100%">Lino-Neto, Teresa</style></author><author><style face="normal" font="default" size="100%">Monteiro, Filipa</style></author><author><style face="normal" font="default" size="100%">Figueiredo, Andreia</style></author><author><style face="normal" font="default" size="100%">Sousa, Lisete</style></author><author><style face="normal" font="default" size="100%">Pais, Maria Salome Salomé</style></author><author><style face="normal" font="default" size="100%">Tavares, Rui</style></author><author><style face="normal" font="default" size="100%">Paulo, Octávio S Octaavio S</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Oak Root Response to Ectomycorrhizal Symbiosis Establishment: RNA-Seq Derived Transcript Identification and Expression Profiling</style></title><secondary-title><style face="normal" font="default" size="100%">PLOS ONE</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">boreal forests</style></keyword><keyword><style  face="normal" font="default" size="100%">ectomycorrhizal symbiosis</style></keyword><keyword><style  face="normal" font="default" size="100%">stress tolerance</style></keyword><keyword><style  face="normal" font="default" size="100%">temperate forests</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year></dates><publisher><style face="normal" font="default" size="100%">PUBLIC LIBRARY SCIENCE</style></publisher><pub-location><style face="normal" font="default" size="100%">1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA</style></pub-location><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">e98376</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Ectomycorrhizal symbiosis is essential for the life and health of trees in temperate and boreal forests where it plays a major role in nutrient cycling and in functioning of the forest ecosystem. Trees with ectomycorrhizal root tips are more tolerant to environmental stresses, such as drought, and biotic stresses such as root pathogens. Detailed information on these molecular processes is essential for the understanding of symbiotic tissue development in order to optimize the benefits of this natural phenomenon. Next generation sequencing tools allow the analysis of non model ectomycorrhizal plant-fungal interactions that can contribute to find the ``symbiosis toolkits'' and better define the role of each partner in the mutualistic interaction. By using 454 pyrosequencing we compared ectomycorrhizal cork oak roots with non-symbiotic roots. From the two cDNA libraries sequenced, over 2 million reads were obtained that generated 19552 cork oak root unique transcripts. A total of 2238 transcripts were found to be differentially expressed when ECM roots were compared with non-symbiotic roots. Identification of up-and down-regulated gens in ectomycorrhizal roots lead to a number of insights into the molecular mechanisms governing this important symbiosis. In cork oak roots, ectomycorrhizal colonization resulted in extensive cell wall remodelling, activation of the secretory pathway, alterations in flavonoid biosynthesis, and expression of genes involved in the recognition of fungal effectors. In addition, we identified genes with putative roles in symbiotic processes such as nutrient exchange with the fungal partner, lateral root formation or root hair decay. These findings provide a global overview of the transcriptome of an ectomycorrhizal host root, and constitute a foundation for future studies on the molecular events controlling this important symbiosis.</style></abstract><accession-num><style face="normal" font="default" size="100%">24859293</style></accession-num><notes><style face="normal" font="default" size="100%">From Duplicate 2 (Oak Root Response to Ectomycorrhizal Symbiosis Establishment: RNA-Seq Derived Transcript Identification and Expression Profiling - Sebastiana, Monica; Vieira, Bruno; Lino-Neto, Teresa; Monteiro, Filipa; Figueiredo, Andreia; Sousa, Lisete; Pais, Maria Salome; Tavares, Rui; Paulo, Octaavio S)</style></notes><research-notes><style face="normal" font="default" size="100%">From Duplicate 2 (Oak Root Response to Ectomycorrhizal Symbiosis Establishment: RNA-Seq Derived Transcript Identification and Expression Profiling - Sebastiana, Monica; Vieira, Bruno; Lino-Neto, Teresa; Monteiro, Filipa; Figueiredo, Andreia; Sousa, Lisete; Pais, Maria Salome; Tavares, Rui; Paulo, Octaavio S)</style></research-notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sebastiana, Mónica</style></author><author><style face="normal" font="default" size="100%">Figueiredo, Andreia</style></author><author><style face="normal" font="default" size="100%">Monteiro, Filipa</style></author><author><style face="normal" font="default" size="100%">Martins, Joana</style></author><author><style face="normal" font="default" size="100%">Franco, Catarina</style></author><author><style face="normal" font="default" size="100%">Coelho, Ana Varela</style></author><author><style face="normal" font="default" size="100%">Vaz, Fátima</style></author><author><style face="normal" font="default" size="100%">Simões, Tânia</style></author><author><style face="normal" font="default" size="100%">Penque, Deborah</style></author><author><style face="normal" font="default" size="100%">Pais, Maria Salomé</style></author><author><style face="normal" font="default" size="100%">Ferreira, Sílvia</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A possible approach for gel-based proteomic studies in recalcitrant woody plants.</style></title><secondary-title><style face="normal" font="default" size="100%">SpringerPlus</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">2-de</style></keyword><keyword><style  face="normal" font="default" size="100%">ectomycorrhizal roots</style></keyword><keyword><style  face="normal" font="default" size="100%">grapevine</style></keyword><keyword><style  face="normal" font="default" size="100%">Mass spectrometry</style></keyword><keyword><style  face="normal" font="default" size="100%">oak</style></keyword><keyword><style  face="normal" font="default" size="100%">pine</style></keyword><keyword><style  face="normal" font="default" size="100%">protein extraction</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year></dates><volume><style face="normal" font="default" size="100%">2</style></volume><pages><style face="normal" font="default" size="100%">210</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Woody plants are particularly difficult to investigate due to high phenolic, resin, and tannin contents and laborious sample preparation. In particular, protein isolation from woody plants for two-dimensional gel electrophoresis (2-DE) is challenging as secondary metabolites negatively interfere with protein extraction and separation. In this study, three protein extraction protocols, using TCA, phenol and ethanol as precipitation or extraction agents, were tested in order to select the more efficient for woody recalcitrant plant gel-based proteomics. Grapevine leaves, pine needles and cork oak ectomycorrhizal roots were used to represent woody plant species and tissues. The phenol protocol produced higher quality 2-DE gels, with increased number of resolved spots, better spot focusing and representation of all molecular mass and isoelectric point ranges tested. In order to test the compatibility of the phenol extracted proteomes with protein identification several spots were excised from the phenol gels and analyzed by mass spectrometry (MALDI-TOF/TOF). Regardless the incomplete genome/protein databases for the plant species under analysis, 49 proteins were identified by Peptide Mass Fingerprint (PMF). Proteomic data have been deposited to the ProteomeXchange with identifier PXD000224. Our results demonstrate the complexity of protein extraction from woody plant tissues and the suitability of the phenol protocol for obtaining high quality protein extracts for efficient 2-DE separation and downstream applications such as protein identification by mass spectrometry.</style></abstract><accession-num><style face="normal" font="default" size="100%">23724367</style></accession-num></record></records></xml>