<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>3</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Romano, A</style></author><author><style face="normal" font="default" size="100%">Noronha, C</style></author><author><style face="normal" font="default" size="100%">Martins-Loução, M a</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Effect of in vitro cold storage on surviving and proliferation of cork-oak (Quercus suber L.) cultures</style></title><secondary-title><style face="normal" font="default" size="100%">Congreso Forestal Español, 14 e 18 Junho de 1993, Lourizán</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cold storage</style></keyword><keyword><style  face="normal" font="default" size="100%">cork-oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Germplasm</style></keyword><keyword><style  face="normal" font="default" size="100%">Medium-term storage</style></keyword><keyword><style  face="normal" font="default" size="100%">micropropagation</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber L.</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1993</style></year></dates><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Cork-oak cultures were stored in vitro without na intervening subculture, at 5º C in the dark for 5 months without loosing their capacity to regenerate when subcultured under standard conditions. The viability, proliferation rate and elongation of the explants were evaluated during 5 months of storage. Proliferation rate of stored cultures was observed during 5 months of storage. Proliferation rate of stored cultures was observed during the following 3 profileration cycles and compared to unstored controls of the same age. Shoot cultures survived with 100% viability and the proliferation rate was not statistically different from the controls. The results here described indicate the feasibility of establishing na in vitro active gene bank for Quercus suber.</style></abstract></record></records></xml>