<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ramos, Miguel</style></author><author><style face="normal" font="default" size="100%">Rocheta, Margarida</style></author><author><style face="normal" font="default" size="100%">Carvalho, Luísa</style></author><author><style face="normal" font="default" size="100%">Inácio, Vera</style></author><author><style face="normal" font="default" size="100%">Graça, José</style></author><author><style face="normal" font="default" size="100%">Morais-Cecílio, Leonor</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Expression of DNA methyltransferases is involved in Quercus suber cork quality</style></title><secondary-title><style face="normal" font="default" size="100%">Tree Genetics &amp; Genomes</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">CMT3</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork quality</style></keyword><keyword><style  face="normal" font="default" size="100%">DMAP1</style></keyword><keyword><style  face="normal" font="default" size="100%">DRM</style></keyword><keyword><style  face="normal" font="default" size="100%">MET1</style></keyword><keyword><style  face="normal" font="default" size="100%">MET2</style></keyword><keyword><style  face="normal" font="default" size="100%">phellogen activity</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year></dates><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">1481-1492</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Abstract Cork oak (Quercus suber ) is an important Portuguese species, mainly due to the economic value of the cork it produces. Cork results from phellogen, a meristematic tissue, which can locally produce lenticels or have discontinu- ities, originating “defects”: pores and nail inclusions that are detrimental to cork industrial use. Epigenetic processes control plant development and its deregulation can lead to altered phenotypes; therefore, the study of epigenetic players in the phellogen is important to understand the emergence of cork's defects. DNA methyltransferases (DNMTs) and one protein associated to MET1 (DMAP1) were characterized in Q. suber, and their gene expression was analyzed in phellogen and con- tiguous differentiating cell layers of trees producing high and low quality cork, after the evaluation of their defects by physical and image analysis methods. All classes of DNMTs (MET, DRM, and CMT) with the respective canonical motifs were identified in Q. suber. The expression analyses of these genes showed that QsDRM2 was the most active methyltransferases in the cells analyzed, and that all the genes were differentially expressed in trees with distinct cork quality,with a tendency for higher expression levels in lowquality producers. Interestingly, the global methylation level was higher in cells with low expression of DNA methyltransferases. A positive and significant correlation was obtained between QsDMAP1 gene expression and the percentage of cork defects. This work provides the first evidence that cork quality in Q. suber is likely influenced by epigenetic mechanisms</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Rocheta, Margarida</style></author><author><style face="normal" font="default" size="100%">Carvalho, Luísa</style></author><author><style face="normal" font="default" size="100%">Viegas, Wanda</style></author><author><style face="normal" font="default" size="100%">Morais-Cecílio, Leonor</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Corky, a gypsy-like retrotransposon is differentially transcribed in Quercus suber tissues.</style></title><secondary-title><style face="normal" font="default" size="100%">BMC research notes</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">corky</style></keyword><keyword><style  face="normal" font="default" size="100%">expression</style></keyword><keyword><style  face="normal" font="default" size="100%">ltr retroelement</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">rt-qpcr</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year></dates><volume><style face="normal" font="default" size="100%">5</style></volume><pages><style face="normal" font="default" size="100%">432</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Background: Transposable elements (TEs) make up a large part of eukaryotic genomes. Due to their repetitive nature and to the fact that they harbour regulatory signals, TEs can be responsible for chromosomal rearrangements, movement of gene sequences and evolution of gene regulation and function. Retrotransposon ubiquity raises the question about their function in genomes and most are transcriptionally inactive due to rearrangements that compromise their activity. However, the activity of TEs is currently considered to have been one of the major processes in genome evolution. Findings: We report on the characterization of a transcriptionally active gypsy-like retrotransposon (named Corky) from Quercus suber, in a comparative and quantitative study of expression levels in different tissues and distinct developmental stages through RT-qPCR. We observed Corky’s differential transcription levels in all the tissues analysed. Conclusions: These results document that Corky’s transcription levels are not constant. Nevertheless, they depend upon the developmental stage, the tissue analysed and the potential occurring events during an individuals’ life span. This modulation brought upon by different developmental and environmental influences suggests an involvement of Corky in stress response and during development.</style></abstract><accession-num><style face="normal" font="default" size="100%">22888907</style></accession-num></record></records></xml>