<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ricardo, Cândido P P</style></author><author><style face="normal" font="default" size="100%">Martins, Isabel</style></author><author><style face="normal" font="default" size="100%">Francisco, Rita</style></author><author><style face="normal" font="default" size="100%">Sergeant, Kjell</style></author><author><style face="normal" font="default" size="100%">Pinheiro, Carla</style></author><author><style face="normal" font="default" size="100%">Campos, Alexandre</style></author><author><style face="normal" font="default" size="100%">Renaut, Jenny</style></author><author><style face="normal" font="default" size="100%">Fevereiro, Pedro</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Proteins associated with cork formation in Quercus suber L. stem tissues.</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of proteomics</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cork</style></keyword><keyword><style  face="normal" font="default" size="100%">Phellem formation</style></keyword><keyword><style  face="normal" font="default" size="100%">Proteomics</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber stem</style></keyword><keyword><style  face="normal" font="default" size="100%">Suberisation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year></dates><publisher><style face="normal" font="default" size="100%">Elsevier B.V.</style></publisher><volume><style face="normal" font="default" size="100%">74</style></volume><pages><style face="normal" font="default" size="100%">1266-1278</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Cork (phellem) formation in Quercus suber stem was studied by proteomic analysis of young shoots of increasing age (Y0, Y1 and Y4) and recently-formed phellem (Y8Ph) and xylem (Y8X) from an 8-year-old branch. In this study 99 proteins were identified, 45 excised from Y8X and 54 from Y8Ph. These ones, specifically associated with phellem, are of &quot;carbohydrate metabolism&quot; (28%), &quot;defence&quot; (22%), &quot;protein folding, stability and degradation&quot; (19%), &quot;regulation/signalling&quot; (11%), &quot;secondary metabolism&quot; (9%), &quot;energy metabolism&quot; (6%), and &quot;membrane transport&quot; (2%). The identification in phellem of galactosidases, xylosidases, apiose/xylose synthase, laccases and diphenol oxidases suggests intense cell wall reorganization, possibly with participation of hemicellulose/pectin biosynthesis and phenol oxidation. The identification of proteasome subunits, heat shock proteins, cyclophylin, subtilisin-like proteases, 14-3-3 proteins, Rab2 protein and enzymes interacting with nucleosides/nucleic acids gives additional evidence for cellular reorganization, involving cellular secretion, protein turnover regulation and active control processes. The high involvement in phellem of defence proteins (thioredoxin-dependent peroxidase, glutathione-S-transferase, SGT1 protein, cystatin, and chitinases) suggests a strong need for cell protection from the intense stressful events occurring in active phellem, namely, desiccation, pests/disease protection, detoxification and cell death. Identically, highly enhanced defence functions were previously reported for potato periderm formation.</style></abstract><accession-num><style face="normal" font="default" size="100%">21320649</style></accession-num></record></records></xml>