<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Correia, Barbara</style></author><author><style face="normal" font="default" size="100%">Valledor, Luis</style></author><author><style face="normal" font="default" size="100%">Meijón, Mónica</style></author><author><style face="normal" font="default" size="100%">Rodriguez, José Luis</style></author><author><style face="normal" font="default" size="100%">Dias, Maria Celeste</style></author><author><style face="normal" font="default" size="100%">Santos, Conceição</style></author><author><style face="normal" font="default" size="100%">Cañal, Maria Jesus</style></author><author><style face="normal" font="default" size="100%">Rodriguez, Roberto</style></author><author><style face="normal" font="default" size="100%">Pinto, Glória</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Is the Interplay between Epigenetic Markers Related to the Acclimation of Cork Oak Plants to High Temperatures?</style></title><secondary-title><style face="normal" font="default" size="100%">PLoS ONE</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Acclimatization</style></keyword><keyword><style  face="normal" font="default" size="100%">Acclimatization: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Acetylation</style></keyword><keyword><style  face="normal" font="default" size="100%">Blotting</style></keyword><keyword><style  face="normal" font="default" size="100%">CHROMATIN</style></keyword><keyword><style  face="normal" font="default" size="100%">CORK oak (citation</style></keyword><keyword><style  face="normal" font="default" size="100%">Deoxycytidine</style></keyword><keyword><style  face="normal" font="default" size="100%">Deoxycytidine: analogs &amp; derivatives</style></keyword><keyword><style  face="normal" font="default" size="100%">Deoxycytidine: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA methylation</style></keyword><keyword><style  face="normal" font="default" size="100%">Electrolytes</style></keyword><keyword><style  face="normal" font="default" size="100%">Electrolytes: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Epigenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene expression</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic Markers</style></keyword><keyword><style  face="normal" font="default" size="100%">Genome</style></keyword><keyword><style  face="normal" font="default" size="100%">Heat-Shock Response</style></keyword><keyword><style  face="normal" font="default" size="100%">Heat-Shock Response: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Histones</style></keyword><keyword><style  face="normal" font="default" size="100%">Histones: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Physiological</style></keyword><keyword><style  face="normal" font="default" size="100%">Physiological: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">POST-translational modification</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Random Amplified Polymorphic DNA Technique</style></keyword><keyword><style  face="normal" font="default" size="100%">Stress</style></keyword><keyword><style  face="normal" font="default" size="100%">tags)</style></keyword><keyword><style  face="normal" font="default" size="100%">Temperature</style></keyword><keyword><style  face="normal" font="default" size="100%">TREES -- Research</style></keyword><keyword><style  face="normal" font="default" size="100%">Western</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2013///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3543447&amp;tool=pmcentrez&amp;rendertype=abstracthttp://dx.doi.org/10.1371/journal.pone.0053543</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">8</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Trees necessarily experience changes in temperature, requiring efficient short-term strategies that become crucial in environmental change adaptability. DNA methylation and histone posttranslational modifications have been shown to play a key role in both epigenetic control and plant functional status under stress by controlling the functional state of chromatin and gene expression. Cork oak (Quercus suber L.) is a key stone of the Mediterranean region, growing at temperatures of 45°C. This species was subjected to a cumulative temperature increase from 25°C to 55°C under laboratory conditions in order to test the hypothesis that epigenetic code is related to heat stress tolerance. Electrolyte leakage increased after 35°C, but all plants survived to 55°C. DNA methylation and acetylated histone H3 (AcH3) levels were monitored by HPCE (high performance capillary electrophoresis), MS-RAPD (methylation-sensitive random-amplified polymorphic DNA) and Protein Gel Blot analysis and the spatial distribution of the modifications was assessed using a confocal microscope. DNA methylation analysed by HPCE revealed an increase at 55°C, while MS-RAPD results pointed to dynamic methylation-demethylation patterns over stress. Protein Gel Blot showed the abundance index of AcH3 decreasing from 25°C to 45°C. The immunohistochemical detection of 5-mC (5-methyl-2′-deoxycytidine) and AcH3 came upon the previous results. These results indicate that epigenetic mechanisms such as DNA methylation and histone H3 acetylation have opposite and particular dynamics that can be crucial for the stepwise establishment of this species into such high stress (55°C), allowing its acclimation and survival. This is the first report that assesses epigenetic regulation in order to investigate heat tolerance in forest trees.</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Public Library of Science&lt;br/&gt;accession-num: 23326451</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Valero-Galvàn, José</style></author><author><style face="normal" font="default" size="100%">González-Fernández, Raquel</style></author><author><style face="normal" font="default" size="100%">Navarro-Cerrillo, Rafael Maria</style></author><author><style face="normal" font="default" size="100%">Gil-Pelegrín, Eustaquio</style></author><author><style face="normal" font="default" size="100%">Jorrín-Novo, Jesús V</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Physiological and Proteomic Analyses of Drought Stress Response in Holm Oak Provenances</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Proteome Research</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Analysis of Variance</style></keyword><keyword><style  face="normal" font="default" size="100%">biomass</style></keyword><keyword><style  face="normal" font="default" size="100%">Chlorophyll ﬂuorescence</style></keyword><keyword><style  face="normal" font="default" size="100%">drought stress in Holm oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Droughts</style></keyword><keyword><style  face="normal" font="default" size="100%">Electrophoresis</style></keyword><keyword><style  face="normal" font="default" size="100%">Gel</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene Expression Regulation</style></keyword><keyword><style  face="normal" font="default" size="100%">Holm oak proteomics</style></keyword><keyword><style  face="normal" font="default" size="100%">Physiological</style></keyword><keyword><style  face="normal" font="default" size="100%">Physiological: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Physiological: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Proteomics</style></keyword><keyword><style  face="normal" font="default" size="100%">Proteomics: methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Spain</style></keyword><keyword><style  face="normal" font="default" size="100%">Stress</style></keyword><keyword><style  face="normal" font="default" size="100%">Tandem mass spectrometry</style></keyword><keyword><style  face="normal" font="default" size="100%">Two-Dimensional</style></keyword><keyword><style  face="normal" font="default" size="100%">water potential</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year></dates><publisher><style face="normal" font="default" size="100%">American Chemical Society</style></publisher><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">5110-5123</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Responses to drought stress by water withholding have been studied in 1 year old Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) seedlings from seven provenances from Andalusia (southern Spain). Several physiological parameters, including predawn xylem water potentials and relative water content in soil, roots, and leaves as well as maximum quantum efficiency and yield of PSII were evaluated for 28 days in both irrigated and nonirrigated seedlings. The leaf proteome map of the two provenances that show the extreme responses (Seville, GSE, is the most susceptible, while Almer??a, SSA, is the least susceptible) was obtained. Statistically significant variable spots among provenances and treatments were subjected to MALDI-TOF/TOF-MS/MS analysis for protein identification. In response to drought stress, ?12.4% of the reproducible spots varied significantly depending on the treatment and the population. These variable proteins were mainly chloroplastic and belonged to the metabolism and defense/stress functional categories. The 2-DE protein profile of nonirrigated seedlings was similar in both provenances. Physiological and proteomics data were generally in good agreement. The general trend was a decrease in protein abundance upon water withholding in both provenances, mainly in those involved in ATP synthesis and photosynthesis. This decrease, moreover, was most marked in the most susceptible population compared with the less susceptible one.</style></abstract><accession-num><style face="normal" font="default" size="100%">24088139</style></accession-num><notes><style face="normal" font="default" size="100%">From Duplicate 2 (Physiological and Proteomic Analyses of Drought Stress Response in Holm Oak Provenances - Valero-Galván, José; González-Fernández, Raquel; Navarro-Cerrillo, Rafael Maria; Gil-Pelegrín, Eustaquio; Jorrín-Novo, Jesús V)</style></notes><research-notes><style face="normal" font="default" size="100%">From Duplicate 2 (Physiological and Proteomic Analyses of Drought Stress Response in Holm Oak Provenances - Valero-Galván, José; González-Fernández, Raquel; Navarro-Cerrillo, Rafael Maria; Gil-Pelegrín, Eustaquio; Jorrín-Novo, Jesús V)</style></research-notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Baldantoni, Daniela</style></author><author><style face="normal" font="default" size="100%">Fagnano, Massimo</style></author><author><style face="normal" font="default" size="100%">Alfani, Anna</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Tropospheric ozone effects on chemical composition and decomposition rate of Quercus ilex L. leaves.</style></title><secondary-title><style face="normal" font="default" size="100%">The Science of the total environment</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Air Pollutants</style></keyword><keyword><style  face="normal" font="default" size="100%">Air Pollutants: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Air Pollutants: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Air Pollutants: toxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">Atmosphere</style></keyword><keyword><style  face="normal" font="default" size="100%">Atmosphere: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Carbon</style></keyword><keyword><style  face="normal" font="default" size="100%">carbon cycle</style></keyword><keyword><style  face="normal" font="default" size="100%">Carbon: analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Carbon: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Cellulose</style></keyword><keyword><style  face="normal" font="default" size="100%">Cellulose: analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Cellulose: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Decaying leaf composition</style></keyword><keyword><style  face="normal" font="default" size="100%">Holm oak leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Lignin</style></keyword><keyword><style  face="normal" font="default" size="100%">Lignin: analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Lignin: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Mediterranean area</style></keyword><keyword><style  face="normal" font="default" size="100%">nitrogen</style></keyword><keyword><style  face="normal" font="default" size="100%">nitrogen cycle</style></keyword><keyword><style  face="normal" font="default" size="100%">Nitrogen: analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Nitrogen: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Ozone</style></keyword><keyword><style  face="normal" font="default" size="100%">ozone exposure</style></keyword><keyword><style  face="normal" font="default" size="100%">Ozone: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Ozone: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Ozone: toxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: metabolism</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year></dates><publisher><style face="normal" font="default" size="100%">Elsevier B.V.</style></publisher><volume><style face="normal" font="default" size="100%">409</style></volume><pages><style face="normal" font="default" size="100%">979-984</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">We determined the effects of tropospheric ozone on the chemical composition of Quercus ilex L. leaves and their decomposition, with a view to assessing the influence of ozone on nutrient cycling and the sustainability of Mediterranean holm oak forests. Forming one of the most widespread thermophilous vegetation communities in the area, Q. ilex is a dominant and widespread evergreen oak in the Mediterranean, where concentrations of tropospheric ozone are particularly high. The dynamics of carbon, nitrogen, lignin and cellulose concentrations were monitored for six months during the decomposition of leaves from plants subjected to controlled ozone exposure in open-top chambers. Ozone-exposed leaves, compared to unexposed leaves, showed no significant differences in C, N, lignin and cellulose concentrations prior to the incubation in mesocosms. However, during decomposition, leaves from plants exposed to ozone lost C significantly more slowly and showed a higher C/N ratio than unexposed leaves. Ozone exposure significantly slowed down the decomposition rate, indicating a negative effect of tropospheric ozone on nutrient cycling, which may reduce long-term sustainability of the holm oak forest.</style></abstract><accession-num><style face="normal" font="default" size="100%">21167557</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sardans, Jordi</style></author><author><style face="normal" font="default" size="100%">Penuelas, Josep</style></author><author><style face="normal" font="default" size="100%">Lope-Piedrafita, Silvia</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Changes in water content and distribution in Quercus ilex leaves during progressive drought assessed by in vivo 1H magnetic resonance imaging.</style></title><secondary-title><style face="normal" font="default" size="100%">BMC plant biology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Droughts</style></keyword><keyword><style  face="normal" font="default" size="100%">Magnetic Resonance Imaging</style></keyword><keyword><style  face="normal" font="default" size="100%">Physiological</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Soil</style></keyword><keyword><style  face="normal" font="default" size="100%">Soil: analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Stress</style></keyword><keyword><style  face="normal" font="default" size="100%">Time Factors</style></keyword><keyword><style  face="normal" font="default" size="100%">water</style></keyword><keyword><style  face="normal" font="default" size="100%">Water: metabolism (citation)</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2010</style></year></dates><publisher><style face="normal" font="default" size="100%">BioMed Central</style></publisher><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">188</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Drought is a common stressor in many regions of the world and current climatic global circulation models predict further increases in warming and drought in the coming decades in several of these regions, such as the Mediterranean basin. The changes in leaf water content, distribution and dynamics in plant tissues under different soil water availabilities are not well known. In order to fill this gap, in the present report we describe our study withholding the irrigation of the seedlings of Quercus ilex, the dominant tree species in the evergreen forests of many areas of the Mediterranean Basin. We have monitored the gradual changes in water content in the different leaf areas, in vivo and non-invasively, by 1H magnetic resonance imaging (MRI) using proton density weighted (rhow) images and spin-spin relaxation time (T2) maps.</style></abstract><accession-num><style face="normal" font="default" size="100%">20735815</style></accession-num><notes><style face="normal" font="default" size="100%">From Duplicate 1 ( Changes in water content and distribution in Quercus ilex leaves during progressive drought assessed by in vivo 1H magnetic resonance imaging. - Sardans, Jordi; Peñuelas, Josep; Lope-Piedrafita, Silvia )</style></notes><research-notes><style face="normal" font="default" size="100%">From Duplicate 1 ( Changes in water content and distribution in Quercus ilex leaves during progressive drought assessed by in vivo 1H magnetic resonance imaging. - Sardans, Jordi; Peñuelas, Josep; Lope-Piedrafita, Silvia )</style></research-notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Grote, Rüdiger</style></author><author><style face="normal" font="default" size="100%">LAVOIR, ANNE-VIOLETTE</style></author><author><style face="normal" font="default" size="100%">Rambal, Serge</style></author><author><style face="normal" font="default" size="100%">Staudt, Michael</style></author><author><style face="normal" font="default" size="100%">Zimmer, Ina</style></author><author><style face="normal" font="default" size="100%">Schnitzler, Jörg-Peter</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Modelling the drought impact on monoterpene fluxes from an evergreen Mediterranean forest canopy.</style></title><secondary-title><style face="normal" font="default" size="100%">Oecologia</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Biological</style></keyword><keyword><style  face="normal" font="default" size="100%">Carbon dioxide</style></keyword><keyword><style  face="normal" font="default" size="100%">Carbon Dioxide: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Computer Simulation</style></keyword><keyword><style  face="normal" font="default" size="100%">Drought impact</style></keyword><keyword><style  face="normal" font="default" size="100%">Droughts</style></keyword><keyword><style  face="normal" font="default" size="100%">France</style></keyword><keyword><style  face="normal" font="default" size="100%">Model coupling</style></keyword><keyword><style  face="normal" font="default" size="100%">Models</style></keyword><keyword><style  face="normal" font="default" size="100%">monoterpene emission</style></keyword><keyword><style  face="normal" font="default" size="100%">Monoterpenes</style></keyword><keyword><style  face="normal" font="default" size="100%">Monoterpenes: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">photosynthesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Photosynthesis: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex (holm oak)</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Scaling</style></keyword><keyword><style  face="normal" font="default" size="100%">Trees</style></keyword><keyword><style  face="normal" font="default" size="100%">Trees: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">water</style></keyword><keyword><style  face="normal" font="default" size="100%">Water: metabolism</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2009</style></year></dates><volume><style face="normal" font="default" size="100%">160</style></volume><pages><style face="normal" font="default" size="100%">213-223</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">In many ecosystems drought cycles are common during the growing season but their impact on volatile monoterpene emissions is unclear. Therefore, we aimed to develop and evaluate a process-based modelling approach to explore the explanatory power of likely mechanisms. The biochemically based isoprene and monoterpene emission model SIM-BIM2 has been modified and linked to a canopy model and a soil water balance model. Simulations are carried out for Quercus ilex forest sites and results are compared to measured soil water, photosynthesis, terpene-synthase activity, and monoterpene emission rates. Finally, the coupled model system is used to estimate the annual drought impact on photosynthesis and emission. The combined and adjusted vegetation model was able to simulate photosynthesis and monoterpene emission under dry and irrigated conditions with an R(2) of 0.74 and 0.52, respectively. We estimated an annual reduction of monoterpene emission of 67% for the extended and severe drought period in 2006 in the investigated Mediterranean ecosystem. It is concluded that process-based ecosystem models can provide a useful tool to investigate the involved mechanisms and to quantify the importance of specific environmental constraints.</style></abstract><accession-num><style face="normal" font="default" size="100%">19219456</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Palacio, Sara</style></author><author><style face="normal" font="default" size="100%">Milla, Rubén</style></author><author><style face="normal" font="default" size="100%">Albuixech, Jorge</style></author><author><style face="normal" font="default" size="100%">Pérez-Rontomé, Carmen</style></author><author><style face="normal" font="default" size="100%">Camarero, Jesús Julio</style></author><author><style face="normal" font="default" size="100%">Maestro, Melchor</style></author><author><style face="normal" font="default" size="100%">Montserrat-Martí, Gabriel</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Seasonal variability of dry matter content and its relationship with shoot growth and nonstructural carbohydrates</style></title><secondary-title><style face="normal" font="default" size="100%">New Phytologist</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Carbohydrate Metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">functional classifications</style></keyword><keyword><style  face="normal" font="default" size="100%">leaf dry matter content (LDMC)</style></keyword><keyword><style  face="normal" font="default" size="100%">leaf traits</style></keyword><keyword><style  face="normal" font="default" size="100%">leaf water status</style></keyword><keyword><style  face="normal" font="default" size="100%">Mediterranean</style></keyword><keyword><style  face="normal" font="default" size="100%">Phenology</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Development</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Shoots</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Shoots: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Shoots: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Stems</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Stems: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Stems: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plants</style></keyword><keyword><style  face="normal" font="default" size="100%">Plants: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Seasons</style></keyword><keyword><style  face="normal" font="default" size="100%">shoot growth</style></keyword><keyword><style  face="normal" font="default" size="100%">Species Specificity</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2008</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2008///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/18643937http://dx.doi.org/10.1111/j.1469-8137.2008.02569.x</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">180</style></volume><pages><style face="normal" font="default" size="100%">133 - 142</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">* • This study assesses how different phases of shoot growth underlie seasonal change in leaf and stem dry matter content (LDMC and SDMC, respectively) of 12 woody Mediterranean species. The relationship between LDMC and nonstructural carbohydrate (NSC) concentrations is also explored and the seasonal vs interspecies variability of LDMC compared. * • LDMC, SDMC and shoot elongation rate (SER) were measured on a monthly basis for a minimum of 12 months. Bud growth rate (BGR) and NSC concentrations were also assessed in several of the study species. * • LDMC and SDMC decreased during shoot elongation in spring and increased in summer, showing a significant negative correlation with SER, but were unrelated to BGR. Half of the species analysed showed a positive relationship between LDMC and NSC. Seasonal fluctuations of LDMC within species were higher than interspecies differences, and species ranking was significantly affected by the month of sampling, except during winter months. * • Seasonal changes in LDMC and SDMC are mainly related to shoot elongation phenology, and NSC sink–source relationships between old and growing organs can explain this relationship in some species. Owing to the high seasonal variability in LDMC, it is recommended that samples for comparative purposes should be collected as close to the winter as possible.</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Blackwell Publishing Ltd&lt;br/&gt;accession-num: 18643937</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Palacio, Sara</style></author><author><style face="normal" font="default" size="100%">Milla, Rubén</style></author><author><style face="normal" font="default" size="100%">Albuixech, Jorge</style></author><author><style face="normal" font="default" size="100%">Pérez-Rontomé, Carmen</style></author><author><style face="normal" font="default" size="100%">Camarero, Jesús Julio</style></author><author><style face="normal" font="default" size="100%">Maestro, Melchor</style></author><author><style face="normal" font="default" size="100%">Montserrat-Martí, Gabriel</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Seasonal variability of dry matter content and its relationship with shoot growth and nonstructural carbohydrates</style></title><secondary-title><style face="normal" font="default" size="100%">New Phytologist</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Carbohydrate Metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">functional classifications</style></keyword><keyword><style  face="normal" font="default" size="100%">leaf dry matter content (LDMC)</style></keyword><keyword><style  face="normal" font="default" size="100%">leaf traits</style></keyword><keyword><style  face="normal" font="default" size="100%">leaf water status</style></keyword><keyword><style  face="normal" font="default" size="100%">Mediterranean</style></keyword><keyword><style  face="normal" font="default" size="100%">Phenology</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Development</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Shoots</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Shoots: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Shoots: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Stems</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Stems: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Stems: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plants</style></keyword><keyword><style  face="normal" font="default" size="100%">Plants: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Seasons</style></keyword><keyword><style  face="normal" font="default" size="100%">shoot growth</style></keyword><keyword><style  face="normal" font="default" size="100%">Species Specificity</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2008</style></year></dates><publisher><style face="normal" font="default" size="100%">Blackwell Publishing Ltd</style></publisher><volume><style face="normal" font="default" size="100%">180</style></volume><pages><style face="normal" font="default" size="100%">133-142</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">* • This study assesses how different phases of shoot growth underlie seasonal change in leaf and stem dry matter content (LDMC and SDMC, respectively) of 12 woody Mediterranean species. The relationship between LDMC and nonstructural carbohydrate (NSC) concentrations is also explored and the seasonal vs interspecies variability of LDMC compared. * • LDMC, SDMC and shoot elongation rate (SER) were measured on a monthly basis for a minimum of 12 months. Bud growth rate (BGR) and NSC concentrations were also assessed in several of the study species. * • LDMC and SDMC decreased during shoot elongation in spring and increased in summer, showing a significant negative correlation with SER, but were unrelated to BGR. Half of the species analysed showed a positive relationship between LDMC and NSC. Seasonal fluctuations of LDMC within species were higher than interspecies differences, and species ranking was significantly affected by the month of sampling, except during winter months. * • Seasonal changes in LDMC and SDMC are mainly related to shoot elongation phenology, and NSC sink–source relationships between old and growing organs can explain this relationship in some species. Owing to the high seasonal variability in LDMC, it is recommended that samples for comparative purposes should be collected as close to the winter as possible.</style></abstract><accession-num><style face="normal" font="default" size="100%">18643937</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Grote, Rüdiger</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Sensitivity of volatile monoterpene emission to changes in canopy structure: a model-based exercise with a process-based emission model</style></title><secondary-title><style face="normal" font="default" size="100%">New Phytologist</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Biological</style></keyword><keyword><style  face="normal" font="default" size="100%">biomass</style></keyword><keyword><style  face="normal" font="default" size="100%">foliage distribution</style></keyword><keyword><style  face="normal" font="default" size="100%">leaf area index</style></keyword><keyword><style  face="normal" font="default" size="100%">light</style></keyword><keyword><style  face="normal" font="default" size="100%">Models</style></keyword><keyword><style  face="normal" font="default" size="100%">monoterpene emission</style></keyword><keyword><style  face="normal" font="default" size="100%">Monoterpenes</style></keyword><keyword><style  face="normal" font="default" size="100%">Monoterpenes: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">photosynthesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Photosynthesis: radiation effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: radiation effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: radiation effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Scaling</style></keyword><keyword><style  face="normal" font="default" size="100%">stand density</style></keyword><keyword><style  face="normal" font="default" size="100%">Temperature</style></keyword><keyword><style  face="normal" font="default" size="100%">Time Factors</style></keyword><keyword><style  face="normal" font="default" size="100%">Volatilization</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2007</style></year></dates><publisher><style face="normal" font="default" size="100%">Blackwell Publishing Ltd</style></publisher><volume><style face="normal" font="default" size="100%">173</style></volume><pages><style face="normal" font="default" size="100%">550-561</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">* • This paper investigates the dependence of monoterpene emissions at the canopy scale on total leaf area and leaf distribution. Simulations were carried out for a range of hypothetical but realistic forest canopies of the evergreen Quercus ilex (holm oak). * • Two emission models were applied that either did (SIM-BIM2) or did not (G93) account for cumulative responses to temperature and light. Both were embedded into a canopy model that considered spatial and temporal variations of foliage properties. This canopy model was coupled to a canopy climate model (CANOAK) to determine the micrometeorological conditions at the leaf scale. * • Structural properties considerably impacted monoterpene emission. The sensitivities to changes in total leaf area and to leaf area distribution were found to be of similar magnitude. The two different models performed similarly on a whole-year basis but showed clear differences during certain episodes. * • The analysis showed that structural indices have to be carefully evaluated for proper scaling of emission from leaves to canopy. Further research is encouraged on seasonal dynamics of emission potentials.</style></abstract><accession-num><style face="normal" font="default" size="100%">17244049</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Filella, Iolanda</style></author><author><style face="normal" font="default" size="100%">Penuelas, Josep</style></author><author><style face="normal" font="default" size="100%">Llusia, Joan</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Dynamics of the enhanced emissions of monoterpenes and methyl salicylate, and decreased uptake of formaldehyde, by Quercus ilex leaves after application of jasmonic acid</style></title><secondary-title><style face="normal" font="default" size="100%">New Phytologist</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Carbon dioxide</style></keyword><keyword><style  face="normal" font="default" size="100%">Carbon Dioxide: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Cyclopentanes</style></keyword><keyword><style  face="normal" font="default" size="100%">Cyclopentanes: pharmacology</style></keyword><keyword><style  face="normal" font="default" size="100%">Dehydration</style></keyword><keyword><style  face="normal" font="default" size="100%">Formaldehyde</style></keyword><keyword><style  face="normal" font="default" size="100%">Formaldehyde: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">jasmonic acid (JA)</style></keyword><keyword><style  face="normal" font="default" size="100%">light</style></keyword><keyword><style  face="normal" font="default" size="100%">methyl salicylate</style></keyword><keyword><style  face="normal" font="default" size="100%">Monoterpenes</style></keyword><keyword><style  face="normal" font="default" size="100%">Monoterpenes: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">net photosynthetic rates</style></keyword><keyword><style  face="normal" font="default" size="100%">Oxylipins</style></keyword><keyword><style  face="normal" font="default" size="100%">photosynthesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex (holm oak)</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Salicylates</style></keyword><keyword><style  face="normal" font="default" size="100%">Salicylates: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Stomatal conductance</style></keyword><keyword><style  face="normal" font="default" size="100%">Temperature</style></keyword><keyword><style  face="normal" font="default" size="100%">VOC (volatile organic compound)</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2006</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2006///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/16390425http://dx.doi.org/10.1111/j.1469-8137.2005.01570.x</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">169</style></volume><pages><style face="normal" font="default" size="100%">135 - 144</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">* • Jasmonic acid (JA) is a signalling compound with a key role in both stress and development in plants, and is reported to elicit the emission of volatile organic compounds (VOCs). Here we studied the dynamics of such emissions and the linkage with photosynthetic rates and stomatal conductance. * • We sprayed JA on leaves of the Mediterranean tree species Quercus ilex and measured the photosynthetic rates, stomatal conductances, and emissions and uptake of VOCs using proton transfer reaction mass spectrometry and gas chromatography after a dark–light transition. * • Jasmonic acid treatment delayed the induction of photosynthesis and stomatal conductance by approx. 20 min, and decreased them 24 h after spraying. Indications were found of both stomatal and nonstomatal limitations of photosynthesis. Monoterpene emissions were enhanced (20–30%) after JA spraying. Jasmonic acid also increased methyl salicylate (MeSa) emissions (more than twofold) 1 h after treatment, although after 24 h this effect had disappeared. Formaldehyde foliar uptake decreased significantly 24 h after JA treatment. * • Both biotic and abiotic stresses can thus affect plant VOC emissions through their strong impact on JA levels. Jasmonic acid-mediated increases in monoterpene and MeSa emissions might have a protective role when confronting biotic and abiotic stresses.</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Blackwell Publishing Ltd&lt;br/&gt;accession-num: 16390425</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Filella, Iolanda</style></author><author><style face="normal" font="default" size="100%">Penuelas, Josep</style></author><author><style face="normal" font="default" size="100%">Llusia, Joan</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Dynamics of the enhanced emissions of monoterpenes and methyl salicylate, and decreased uptake of formaldehyde, by Quercus ilex leaves after application of jasmonic acid</style></title><secondary-title><style face="normal" font="default" size="100%">New Phytologist</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Carbon dioxide</style></keyword><keyword><style  face="normal" font="default" size="100%">Carbon Dioxide: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Cyclopentanes</style></keyword><keyword><style  face="normal" font="default" size="100%">Cyclopentanes: pharmacology</style></keyword><keyword><style  face="normal" font="default" size="100%">Dehydration</style></keyword><keyword><style  face="normal" font="default" size="100%">Formaldehyde</style></keyword><keyword><style  face="normal" font="default" size="100%">Formaldehyde: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">jasmonic acid (JA)</style></keyword><keyword><style  face="normal" font="default" size="100%">light</style></keyword><keyword><style  face="normal" font="default" size="100%">methyl salicylate</style></keyword><keyword><style  face="normal" font="default" size="100%">Monoterpenes</style></keyword><keyword><style  face="normal" font="default" size="100%">Monoterpenes: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">net photosynthetic rates</style></keyword><keyword><style  face="normal" font="default" size="100%">Oxylipins</style></keyword><keyword><style  face="normal" font="default" size="100%">photosynthesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex (holm oak)</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Salicylates</style></keyword><keyword><style  face="normal" font="default" size="100%">Salicylates: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Stomatal conductance</style></keyword><keyword><style  face="normal" font="default" size="100%">Temperature</style></keyword><keyword><style  face="normal" font="default" size="100%">VOC (volatile organic compound)</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2006</style></year></dates><publisher><style face="normal" font="default" size="100%">Blackwell Publishing Ltd</style></publisher><volume><style face="normal" font="default" size="100%">169</style></volume><pages><style face="normal" font="default" size="100%">135-144</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">* • Jasmonic acid (JA) is a signalling compound with a key role in both stress and development in plants, and is reported to elicit the emission of volatile organic compounds (VOCs). Here we studied the dynamics of such emissions and the linkage with photosynthetic rates and stomatal conductance. * • We sprayed JA on leaves of the Mediterranean tree species Quercus ilex and measured the photosynthetic rates, stomatal conductances, and emissions and uptake of VOCs using proton transfer reaction mass spectrometry and gas chromatography after a dark–light transition. * • Jasmonic acid treatment delayed the induction of photosynthesis and stomatal conductance by approx. 20 min, and decreased them 24 h after spraying. Indications were found of both stomatal and nonstomatal limitations of photosynthesis. Monoterpene emissions were enhanced (20–30%) after JA spraying. Jasmonic acid also increased methyl salicylate (MeSa) emissions (more than twofold) 1 h after treatment, although after 24 h this effect had disappeared. Formaldehyde foliar uptake decreased significantly 24 h after JA treatment. * • Both biotic and abiotic stresses can thus affect plant VOC emissions through their strong impact on JA levels. Jasmonic acid-mediated increases in monoterpene and MeSa emissions might have a protective role when confronting biotic and abiotic stresses.</style></abstract><accession-num><style face="normal" font="default" size="100%">16390425</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Jorge, Inmaculada</style></author><author><style face="normal" font="default" size="100%">Navarro, Rafael M</style></author><author><style face="normal" font="default" size="100%">Lenz, Christof</style></author><author><style face="normal" font="default" size="100%">Ariza, David</style></author><author><style face="normal" font="default" size="100%">Jorrín, Jesús</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Variation in the holm oak leaf proteome at different plant developmental stages, between provenances and in response to drought stress.</style></title><secondary-title><style face="normal" font="default" size="100%">Proteomics</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Dehydration</style></keyword><keyword><style  face="normal" font="default" size="100%">Dehydration: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Disasters</style></keyword><keyword><style  face="normal" font="default" size="100%">Electrophoresis, Gel, Two-Dimensional</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Proteome</style></keyword><keyword><style  face="normal" font="default" size="100%">Proteome: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Proteomics</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: embryology</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling: metabolism</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2006</style></year></dates><volume><style face="normal" font="default" size="100%">6 Suppl 1</style></volume><pages><style face="normal" font="default" size="100%">S207-14</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Major proteins of the holm oak leaf proteome have been previously identified using a combination of 2-DE, MS analysis and BLAST similarity search (Jorge et al., Proteomics 2005, 5, 222-234). That study, conducted with field samples from mature trees, revealed the existence of a great variability in the 2-DE protein map, with qualitative as well as quantitative changes, both analytical and biological. A similar study has been carried out with 2-year-old seedlings to analyze and study: (i) changes in the 2-DE protein profile at different tree developmental stages; (ii) the 2-DE protein map variability between three different Spanish provenances; and (iii) variations in the 2-DE protein profile in response to drought stress. Although the protein profile of leaves from seedlings and mature trees was fairly similar, the biological variance found was lower in the former. In the present study, new proteins have been identified. At least four different protein spots differentiated Spanish provenances, two of them identified as an ATP synthase alpha chain, and a 2,3-bisphosphoglycerate-independent phosphoglycerate mutase. Fourteen different protein spots were qualitatively variable between well-watered and drought-stressed seedlings, with some of them corresponding to enzymes of carbohydrate and protein metabolism. Data presented indicated the mobilization of storage proteins and carbohydrates, as well as photosynthesis inhibition under drought conditions.</style></abstract><accession-num><style face="normal" font="default" size="100%">16534744</style></accession-num></record></records></xml>