<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>5</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Gómez-Garay, Arancha</style></author><author><style face="normal" font="default" size="100%">Bueno, Ángeles</style></author><author><style face="normal" font="default" size="100%">Pintos, Beatriz</style></author></authors><secondary-authors><author><style face="normal" font="default" size="100%">Kantartzi, Stella K.</style></author></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Identification of DNA-Microsatellite Markers for the Characterization of Somatic Embryos in Quercus suber</style></title><secondary-title><style face="normal" font="default" size="100%">Microsatellites SE - 8</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Clonal lines</style></keyword><keyword><style  face="normal" font="default" size="100%">Doubled haploid</style></keyword><keyword><style  face="normal" font="default" size="100%">Dyes</style></keyword><keyword><style  face="normal" font="default" size="100%">Fingerprint</style></keyword><keyword><style  face="normal" font="default" size="100%">Germplasm</style></keyword><keyword><style  face="normal" font="default" size="100%">Haploid</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2013///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1007/978-1-62703-389-3_8</style></url></web-urls></urls><publisher><style face="normal" font="default" size="100%">Humana Press</style></publisher><volume><style face="normal" font="default" size="100%">1006</style></volume><pages><style face="normal" font="default" size="100%">121 - 129</style></pages><isbn><style face="normal" font="default" size="100%">978-1-62703-388-6</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Nuclear DNA-microsatellite markers led the possibility to characterize individually both Quercus suber trees and somatic embryos. The genotype inferred by SSR markers opens the possibility to obtain a fingerprint for clonal lines identification. Furthermore, allow to infer the origin of somatic embryos from haploid cells (microspores) or from diploid tissues. Using few SSR markers from other Quercus species and an automatic system based in fluorescence, it is possible to obtain a high discrimination power between genotypes. This method is sufficient to assign tissues to an individual tree with high statistical certainty. Nevertheless, it is necessary to take care to select the adequate DNA extraction method to avoid PCR inhibitors present in diverse Q. suber tissues.</style></abstract><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;periodical: Microsatellites SE - 8&lt;br/&gt;electronic-resource-num: 10.1007/978-1-62703-389-3_8</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>3</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Romano, A</style></author><author><style face="normal" font="default" size="100%">Noronha, C</style></author><author><style face="normal" font="default" size="100%">Martins-Loução, M a</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Effect of in vitro cold storage on surviving and proliferation of cork-oak (Quercus suber L.) cultures</style></title><secondary-title><style face="normal" font="default" size="100%">Congreso Forestal Español, 14 e 18 Junho de 1993, Lourizán</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cold storage</style></keyword><keyword><style  face="normal" font="default" size="100%">cork-oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Germplasm</style></keyword><keyword><style  face="normal" font="default" size="100%">Medium-term storage</style></keyword><keyword><style  face="normal" font="default" size="100%">micropropagation</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber L.</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1993</style></year></dates><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Cork-oak cultures were stored in vitro without na intervening subculture, at 5º C in the dark for 5 months without loosing their capacity to regenerate when subcultured under standard conditions. The viability, proliferation rate and elongation of the explants were evaluated during 5 months of storage. Proliferation rate of stored cultures was observed during 5 months of storage. Proliferation rate of stored cultures was observed during the following 3 profileration cycles and compared to unstored controls of the same age. Shoot cultures survived with 100% viability and the proliferation rate was not statistically different from the controls. The results here described indicate the feasibility of establishing na in vitro active gene bank for Quercus suber.</style></abstract></record></records></xml>