<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Alves, Sofia</style></author><author><style face="normal" font="default" size="100%">Ribeiro, Teresa</style></author><author><style face="normal" font="default" size="100%">Inácio, Vera</style></author><author><style face="normal" font="default" size="100%">Rocheta, Margarida</style></author><author><style face="normal" font="default" size="100%">Morais-Cecílio, Leonor</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Genomic organization and dynamics of repetitive DNA sequences in representatives of three Fagaceae genera</style></title><secondary-title><style face="normal" font="default" size="100%">Genome</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">AFLP</style></keyword><keyword><style  face="normal" font="default" size="100%">Castanea</style></keyword><keyword><style  face="normal" font="default" size="100%">euchromatin</style></keyword><keyword><style  face="normal" font="default" size="100%">Fagaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">Fagus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">retroelements</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2012///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1139/g2012-020</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">55</style></volume><pages><style face="normal" font="default" size="100%">348 - 359</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Oaks, chestnuts, and beeches are economically important species of the Fagaceae. To understand the relationship between these members of this family, a deep knowledge of their genome composition and organization is needed. In this work, we have isolated and characterized several AFLP fragments obtained from Quercus rotundifolia Lam. through homology searches in available databases. Genomic polymorphisms involving some of these sequences were evaluated in two species of Quercus, one of Castanea, and one of Fagus with specific primers. Comparative FISH analysis with generated sequences was performed in interphase nuclei of the four species, and the co-immunolocalization of 5-methylcytosine was also studied. Some of the sequences isolated proved to be genus-specific, while others were present in all the genera. Retroelements, either gypsy-like of the Tat/Athila clade or copia-like, are well represented, and most are dispersed in euchromatic regions of these species with no DNA methylation associated, pointing to an interspersed arrangement of these retroelements with potential gene-rich regions. A particular gypsy-sequence is dispersed in oaks and chestnut nuclei, but its confinement to chromocenters in beech evidences genome restructuring events during evolution of Fagaceae. Several sequences generated in this study proved to be good tools to comparatively study Fagaceae genome organization.</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><notes><style face="normal" font="default" size="100%">doi: 10.1139/g2012-020doi: 10.1139/g2012-020The following values have no corresponding Zotero field:&lt;br/&gt;publisher: NRC Research Press</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ribeiro, Teresa</style></author><author><style face="normal" font="default" size="100%">Loureiro, João</style></author><author><style face="normal" font="default" size="100%">Santos, Conceição</style></author><author><style face="normal" font="default" size="100%">Morais-Cecílio, Leonor</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Evolution of rDNA FISH patterns in the Fagaceae</style></title><secondary-title><style face="normal" font="default" size="100%">Tree Genetics &amp; Genomes</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Castanea</style></keyword><keyword><style  face="normal" font="default" size="100%">fagaceae karyotype</style></keyword><keyword><style  face="normal" font="default" size="100%">Fagus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">rDNA</style></keyword><keyword><style  face="normal" font="default" size="100%">triploid quercus suber</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year></dates><volume><style face="normal" font="default" size="100%">7</style></volume><pages><style face="normal" font="default" size="100%">1113-1122</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The Fagaceae is one of the most important plant families in European forest ecosystems, and it includes several genera distributed in the Northern hemisphere. In this work we studied the genome organization and evolution within the family, by karyotyping and physically mapping rDNA in ten European and Asian species of the genera Fagus, Quercus, and Castanea. All of the species studied had a chromosome number of 2n=2x=24, except for the first report of a single individual of Quercus suber which proved to be triploid (2n=3x=36). The rDNA physical mapping revealed several patterns: the dominant one is present in European and Asian Quercus subgenus Quercus, and in Castanea sativa and Castanea crenata, consisting of two 18S–25S rDNA loci (one subterminal major and one pericentromeric minor) and one 5S rDNA pericentromeric locus. In Fagus sylvatica and in Quercus sessilifolia, different patterns were observed: four terminal 18S–25S rDNA loci and two 5S rDNA pericentromeric loci in the former, and five 18S–25S rDNA loci (three terminal and two intercalary) and one 5S rDNA pericentromeric locus in the latter. In Castanea mollissima a distinct rDNA distribution pattern with two intercalary 18S–25S rDNA loci and two 5S rDNA was found. These findings suggest rDNA loci restructuring during Castanea evolution, and variability of 18S–25S loci between Quercus and Cyclobalanopsis subgenera.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Samuel, Rosabelle</style></author><author><style face="normal" font="default" size="100%">Bachmair, Andreas</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">ITS sequences from nuclear rDNA suggest unexpected phylogenetic relationships between Euro-Mediterranean, East Asiatic and North American taxa ofQuercus (</style></title><secondary-title><style face="normal" font="default" size="100%">Plant systematics and …</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Castanea</style></keyword><keyword><style  face="normal" font="default" size="100%">Fagaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">Fagus. - Nuclear rDNA</style></keyword><keyword><style  face="normal" font="default" size="100%">ITS</style></keyword><keyword><style  face="normal" font="default" size="100%">molecular phylogeny</style></keyword><keyword><style  face="normal" font="default" size="100%">molecular systematics</style></keyword><keyword><style  face="normal" font="default" size="100%">N Hemisphere</style></keyword><keyword><style  face="normal" font="default" size="100%">Phytogeography</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1998</style></year></dates><volume><style face="normal" font="default" size="100%">211</style></volume><pages><style face="normal" font="default" size="100%">129-139</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Nucleotide sequences from the internal transcribed spacer (ITS) regions of the 18S-26S nuclear ribosomal DNA have been studied from ten species of Quercus (representing four subgenera), Castanea sativa and Fagus sylvatica, as a preliminary molecular contribution to the still poorly understood systematics and evolution of Fagaceae. The resulting matrix has been used to calculate pair-wise sequence divergence indices and to construct a maximum parsimony tree for Quercus coding indels as a fifth state. Divergence is greater for Quercus vs. Fagus than for Quercus vs. Castanea. The tree for the Quercus taxa studied reveals two clearly divergent clades. In clade I the evergreen W Mediterranean Q. suber appears in a basal position as sister to more distal deciduous taxa, i.e. the E Mediterranean Q. macrolepis and the E Asiatic Q. acutissima (all formerly united as different sections under the apparently polyphyletic subg. Cerris), and Q. rubra (a representative of the N American subg. Erythrobalanus), forming a pair with Q. acutissima. In clade II the evergreen southeastem N American Q. virginiana is basal and sister to the remaining three branches, i.e. a pair of evergreen Mediterranean taxa with Q. ilex and Q. coccifera (subg. Sclerophyllodrys), the deciduous but otherwise plesiomorphic SE European/SW Asiatic Q. cerris (type species of subg. Cerris), and the related but more apomorphic European pair Q. petraea and Q. robur (subg. Quercus). These results partly conflict with current taxonomic classification but a_re supported by some anatomical and morphological characters. They document polyphyletic lines from evergreen to deciduous taxa and suggest Tertiary transcontinental connections within the genus.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">COLLADA, C</style></author><author><style face="normal" font="default" size="100%">CABALLERO, R G</style></author><author><style face="normal" font="default" size="100%">CASADO, R</style></author><author><style face="normal" font="default" size="100%">ARAGONCILLO, C</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">SEED STORAGE PROTEINS IN FAGACEAE - SIMILARITY BETWEEN CASTANEA GLOBULINS AND QUERCUS GLUTELINS</style></title><secondary-title><style face="normal" font="default" size="100%">PLANT SCIENCE</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">11S GLOBULINS</style></keyword><keyword><style  face="normal" font="default" size="100%">Castanea</style></keyword><keyword><style  face="normal" font="default" size="100%">GLUTELINS</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1991</style></year></dates><publisher><style face="normal" font="default" size="100%">ELSEVIER SCI IRELAND LTD</style></publisher><pub-location><style face="normal" font="default" size="100%">CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND</style></pub-location><volume><style face="normal" font="default" size="100%">75</style></volume><pages><style face="normal" font="default" size="100%">145-154</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Castanea sativa Mill. and C. crenata Lieb. and Zucc. store 11S globulins as major seed proteins while Quercus ilex L. and Q. robur L., also members of the Fagaceae family, accumulate glutelins. The structure of the 11S Castanea globulins, which is similar to the accepted model for this class of proteins, has been studied by different two-dimensional electrophoretic methods. The oligomer has a M(r) of 240 000-260 000, and the pairs of acidic-basic subunits, which form it, show great heterogeneity. The Quercus glutelins are made up of dimers linked by disulphide bridges, and their characteristics indicate a similarity to the subunits of 11S Castanea globulins. We suggest that, as in the case of rice glutelins, Quercus glutelins can also be considered legumin-like proteins.</style></abstract></record></records></xml>