<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Valero-Galvàn, José</style></author><author><style face="normal" font="default" size="100%">González-Fernández, Raquel</style></author><author><style face="normal" font="default" size="100%">Navarro-Cerrillo, Rafael Maria</style></author><author><style face="normal" font="default" size="100%">Gil-Pelegrín, Eustaquio</style></author><author><style face="normal" font="default" size="100%">Jorrín-Novo, Jesús V</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Physiological and Proteomic Analyses of Drought Stress Response in Holm Oak Provenances</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Proteome Research</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Analysis of Variance</style></keyword><keyword><style  face="normal" font="default" size="100%">biomass</style></keyword><keyword><style  face="normal" font="default" size="100%">Chlorophyll ﬂuorescence</style></keyword><keyword><style  face="normal" font="default" size="100%">drought stress in Holm oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Droughts</style></keyword><keyword><style  face="normal" font="default" size="100%">Electrophoresis</style></keyword><keyword><style  face="normal" font="default" size="100%">Gel</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene Expression Regulation</style></keyword><keyword><style  face="normal" font="default" size="100%">Holm oak proteomics</style></keyword><keyword><style  face="normal" font="default" size="100%">Physiological</style></keyword><keyword><style  face="normal" font="default" size="100%">Physiological: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Physiological: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Proteomics</style></keyword><keyword><style  face="normal" font="default" size="100%">Proteomics: methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Spain</style></keyword><keyword><style  face="normal" font="default" size="100%">Stress</style></keyword><keyword><style  face="normal" font="default" size="100%">Tandem mass spectrometry</style></keyword><keyword><style  face="normal" font="default" size="100%">Two-Dimensional</style></keyword><keyword><style  face="normal" font="default" size="100%">water potential</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year></dates><publisher><style face="normal" font="default" size="100%">American Chemical Society</style></publisher><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">5110-5123</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Responses to drought stress by water withholding have been studied in 1 year old Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) seedlings from seven provenances from Andalusia (southern Spain). Several physiological parameters, including predawn xylem water potentials and relative water content in soil, roots, and leaves as well as maximum quantum efficiency and yield of PSII were evaluated for 28 days in both irrigated and nonirrigated seedlings. The leaf proteome map of the two provenances that show the extreme responses (Seville, GSE, is the most susceptible, while Almer??a, SSA, is the least susceptible) was obtained. Statistically significant variable spots among provenances and treatments were subjected to MALDI-TOF/TOF-MS/MS analysis for protein identification. In response to drought stress, ?12.4% of the reproducible spots varied significantly depending on the treatment and the population. These variable proteins were mainly chloroplastic and belonged to the metabolism and defense/stress functional categories. The 2-DE protein profile of nonirrigated seedlings was similar in both provenances. Physiological and proteomics data were generally in good agreement. The general trend was a decrease in protein abundance upon water withholding in both provenances, mainly in those involved in ATP synthesis and photosynthesis. This decrease, moreover, was most marked in the most susceptible population compared with the less susceptible one.</style></abstract><accession-num><style face="normal" font="default" size="100%">24088139</style></accession-num><notes><style face="normal" font="default" size="100%">From Duplicate 2 (Physiological and Proteomic Analyses of Drought Stress Response in Holm Oak Provenances - Valero-Galván, José; González-Fernández, Raquel; Navarro-Cerrillo, Rafael Maria; Gil-Pelegrín, Eustaquio; Jorrín-Novo, Jesús V)</style></notes><research-notes><style face="normal" font="default" size="100%">From Duplicate 2 (Physiological and Proteomic Analyses of Drought Stress Response in Holm Oak Provenances - Valero-Galván, José; González-Fernández, Raquel; Navarro-Cerrillo, Rafael Maria; Gil-Pelegrín, Eustaquio; Jorrín-Novo, Jesús V)</style></research-notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sardans, Jordi</style></author><author><style face="normal" font="default" size="100%">Penuelas, Josep</style></author><author><style face="normal" font="default" size="100%">Lope-Piedrafita, Silvia</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Changes in water content and distribution in Quercus ilex leaves during progressive drought assessed by in vivo 1H magnetic resonance imaging.</style></title><secondary-title><style face="normal" font="default" size="100%">BMC plant biology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Droughts</style></keyword><keyword><style  face="normal" font="default" size="100%">Magnetic Resonance Imaging</style></keyword><keyword><style  face="normal" font="default" size="100%">Physiological</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Soil</style></keyword><keyword><style  face="normal" font="default" size="100%">Soil: analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Stress</style></keyword><keyword><style  face="normal" font="default" size="100%">Time Factors</style></keyword><keyword><style  face="normal" font="default" size="100%">water</style></keyword><keyword><style  face="normal" font="default" size="100%">Water: metabolism (citation)</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2010</style></year></dates><publisher><style face="normal" font="default" size="100%">BioMed Central</style></publisher><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">188</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Drought is a common stressor in many regions of the world and current climatic global circulation models predict further increases in warming and drought in the coming decades in several of these regions, such as the Mediterranean basin. The changes in leaf water content, distribution and dynamics in plant tissues under different soil water availabilities are not well known. In order to fill this gap, in the present report we describe our study withholding the irrigation of the seedlings of Quercus ilex, the dominant tree species in the evergreen forests of many areas of the Mediterranean Basin. We have monitored the gradual changes in water content in the different leaf areas, in vivo and non-invasively, by 1H magnetic resonance imaging (MRI) using proton density weighted (rhow) images and spin-spin relaxation time (T2) maps.</style></abstract><accession-num><style face="normal" font="default" size="100%">20735815</style></accession-num><notes><style face="normal" font="default" size="100%">From Duplicate 1 ( Changes in water content and distribution in Quercus ilex leaves during progressive drought assessed by in vivo 1H magnetic resonance imaging. - Sardans, Jordi; Peñuelas, Josep; Lope-Piedrafita, Silvia )</style></notes><research-notes><style face="normal" font="default" size="100%">From Duplicate 1 ( Changes in water content and distribution in Quercus ilex leaves during progressive drought assessed by in vivo 1H magnetic resonance imaging. - Sardans, Jordi; Peñuelas, Josep; Lope-Piedrafita, Silvia )</style></research-notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Villar-Salvador, Pedro</style></author><author><style face="normal" font="default" size="100%">Heredia, Norberto</style></author><author><style face="normal" font="default" size="100%">Millard, Peter</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Remobilization of acorn nitrogen for seedling growth in holm oak (Quercus ilex), cultivated with contrasting nutrient availability.</style></title><secondary-title><style face="normal" font="default" size="100%">Tree physiology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">germination</style></keyword><keyword><style  face="normal" font="default" size="100%">nitrogen</style></keyword><keyword><style  face="normal" font="default" size="100%">Nitrogen Isotopes</style></keyword><keyword><style  face="normal" font="default" size="100%">Nitrogen Isotopes: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Nitrogen: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Seeds</style></keyword><keyword><style  face="normal" font="default" size="100%">Seeds: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Soil</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2010</style></year></dates><volume><style face="normal" font="default" size="100%">30</style></volume><pages><style face="normal" font="default" size="100%">257-63</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The relative contribution of nitrogen (N) reserves from seeds or uptake by the roots to the growth and N content of young seedlings has received little attention. In this study, we investigated the contribution of N from the acorn or uptake by the roots to the N content of holm oak (Quercus ilex L.) seedlings and determined if remobilization of acorn N was affected by nutrient availability in the growing media. Q. ilex seedlings were cultivated for 3 months, until the end of the second shoot flush of growth, with three N fertilization rates: 8.6 mM N, 1.4 mM N or no fertilization. Fertilizer N was enriched in (15)N. Between 62 and 75% of the N contained in high and low fertilized seedlings, respectively, at the end of the second flush of growth was derived from the acorn. However, the dependence on acorn N was greater during the early root growth and first shoot flush of growth and decreased during the second shoot flush of growth, with root uptake contributing 32-54% of plant new N in this latter developmental stage in high and low fertilized plants, respectively. Fertilization rate did not affect the amount of N taken up during the earliest developmental stages, but it increased it during the second shoot flush of growth. Fertilization increased the mass of the shoot segment formed during the second shoot flush of growth and reduced the root mass, with no effect on whole plant growth. Remobilization of acorn N was faster in unfertilized plants than in fertilized plants. It is concluded that the holm oak seedlings depend greatly upon acorn N until the end of the second shoot flush of growth, that significant root N uptake starts at the beginning of the second shoot flush of growth and that acorn N remobilization is a plastic process that is accelerated under extremely low substratum nutrient content.</style></abstract><accession-num><style face="normal" font="default" size="100%">20022863</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Jorge, Inmaculada</style></author><author><style face="normal" font="default" size="100%">Navarro, Rafael M</style></author><author><style face="normal" font="default" size="100%">Lenz, Christof</style></author><author><style face="normal" font="default" size="100%">Ariza, David</style></author><author><style face="normal" font="default" size="100%">Jorrín, Jesús</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Variation in the holm oak leaf proteome at different plant developmental stages, between provenances and in response to drought stress.</style></title><secondary-title><style face="normal" font="default" size="100%">Proteomics</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Dehydration</style></keyword><keyword><style  face="normal" font="default" size="100%">Dehydration: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Disasters</style></keyword><keyword><style  face="normal" font="default" size="100%">Electrophoresis, Gel, Two-Dimensional</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Proteome</style></keyword><keyword><style  face="normal" font="default" size="100%">Proteome: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Proteomics</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: embryology</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling: metabolism</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2006</style></year></dates><volume><style face="normal" font="default" size="100%">6 Suppl 1</style></volume><pages><style face="normal" font="default" size="100%">S207-14</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Major proteins of the holm oak leaf proteome have been previously identified using a combination of 2-DE, MS analysis and BLAST similarity search (Jorge et al., Proteomics 2005, 5, 222-234). That study, conducted with field samples from mature trees, revealed the existence of a great variability in the 2-DE protein map, with qualitative as well as quantitative changes, both analytical and biological. A similar study has been carried out with 2-year-old seedlings to analyze and study: (i) changes in the 2-DE protein profile at different tree developmental stages; (ii) the 2-DE protein map variability between three different Spanish provenances; and (iii) variations in the 2-DE protein profile in response to drought stress. Although the protein profile of leaves from seedlings and mature trees was fairly similar, the biological variance found was lower in the former. In the present study, new proteins have been identified. At least four different protein spots differentiated Spanish provenances, two of them identified as an ATP synthase alpha chain, and a 2,3-bisphosphoglycerate-independent phosphoglycerate mutase. Fourteen different protein spots were qualitatively variable between well-watered and drought-stressed seedlings, with some of them corresponding to enzymes of carbohydrate and protein metabolism. Data presented indicated the mobilization of storage proteins and carbohydrates, as well as photosynthesis inhibition under drought conditions.</style></abstract><accession-num><style face="normal" font="default" size="100%">16534744</style></accession-num></record></records></xml>