<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sardans, J.</style></author><author><style face="normal" font="default" size="100%">Gargallo-Garriga, A.</style></author><author><style face="normal" font="default" size="100%">Pérez-Trujillo, M.</style></author><author><style face="normal" font="default" size="100%">Parella, T. J.</style></author><author><style face="normal" font="default" size="100%">Seco, R.</style></author><author><style face="normal" font="default" size="100%">Filella, I.</style></author><author><style face="normal" font="default" size="100%">Penuelas, J.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Metabolic responses of Quercus ilex seedlings to wounding analysed with nuclear magnetic resonance profiling</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Biology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Asparagine</style></keyword><keyword><style  face="normal" font="default" size="100%">choline</style></keyword><keyword><style  face="normal" font="default" size="100%">glucose</style></keyword><keyword><style  face="normal" font="default" size="100%">herbivory</style></keyword><keyword><style  face="normal" font="default" size="100%">NMR metabolomics</style></keyword><keyword><style  face="normal" font="default" size="100%">quercitol</style></keyword><keyword><style  face="normal" font="default" size="100%">quinic acid</style></keyword><keyword><style  face="normal" font="default" size="100%">wounding</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2013///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1111/plb.12032</style></url></web-urls></urls><pages><style face="normal" font="default" size="100%">n/a - n/a</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Plants defend themselves against herbivory at several levels. One of these is the synthesis of inducible chemical defences. Using NMR metabolomic techniques, we studied the metabolic changes of plant leaves after a wounding treatment simulating herbivore attack in the Mediterranean sclerophyllous tree Quercus ilex. First, an increase in glucose content was observed in wounded plants. There was also an increase in the content of C-rich secondary metabolites such as quinic acid and quercitol, both related to the shikimic acid pathway and linked to defence against biotic stress. There was also a shift in N-storing amino acids, from leucine and isoleucine to asparagine and choline. The observed higher content of asparagine is related to the higher content of choline through serine that was proved to be the precursor of choline. Choline is a general anti-herbivore and pathogen deterrent. The study shows the rapid metabolic response of Q. ilex in defending its leaves, based on a rapid increase in the production of quinic acid, quercitol and choline. The results also confirm the suitability of 1H NMR-based metabolomic profiling studies to detect global metabolome shifts after wounding stress in tree leaves, and therefore its suitability in ecometabolomic studies.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">SILLA, F.</style></author><author><style face="normal" font="default" size="100%">Escudero, A.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Nitrogen-use efficiency: trade-offs between N productivity and mean residence time at organ, plant and population levels</style></title><secondary-title><style face="normal" font="default" size="100%">Functional Ecology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Drought stress</style></keyword><keyword><style  face="normal" font="default" size="100%">herbivory</style></keyword><keyword><style  face="normal" font="default" size="100%">nitrogen loss</style></keyword><keyword><style  face="normal" font="default" size="100%">nitrogen uptake</style></keyword><keyword><style  face="normal" font="default" size="100%">plant strategies</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2004</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2004///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1111/j.0269-8463.2004.00872.x</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">18</style></volume><pages><style face="normal" font="default" size="100%">511 - 521</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">* 1Nitrogen-use efficiency (NUEN) is often decomposed into the product of N productivity (AN) and the mean residence time of N (MRTN). Theory suggests a trade-off between both components, but direct experimental evidence is still scarce. A field study with young trees of the evergreen Quercus ilex and the marcescent-evergreen Quercus faginea was carried out to test this trade-off through analysis of plant traits at organ, whole-plant and population levels. * 2Specific leaf area (SLA) was the main trait positively related to AN in Q. faginea. By contrast, greater litter production and consumption by caterpillars resulted in larger N losses and shorter MRTN in Q. faginea. Early leaf senescence in Q. faginea produced leaf litter with high N concentration that contributed significantly to N loss. Moreover, Q. ilex had higher plant survivorship. The inverse relationship between leaf longevity and SLA is probably a key component of the trade-off between N losses and plant N productivity. * 3Quercus faginea had greater N uptake from soil, linked to its longer specific root length of fine roots and greater biomass allocation to underground tissues. Smaller N losses in Q. ilex compensated for its smaller N uptake and allowed a similar N balance at whole-plant level. * 4Our results support the hypothesis of a trade-off between AN and MRTN. Quercus ilex had a long MRTN, while Q. faginea has a high AN, and vice versa. The long MRTN in Q. ilex involves not only reduced N loss through long intrinsic leaf life span, but also resistance to harsh environmental factors and defence against herbivores. This suggests that a long MRTN is a potentially successful strategy in nutrient-poor environments.</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Blackwell Science Ltd</style></notes></record></records></xml>