<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Bragança, Helena</style></author><author><style face="normal" font="default" size="100%">Rigling, Daniel</style></author><author><style face="normal" font="default" size="100%">Diogo, Eugénio</style></author><author><style face="normal" font="default" size="100%">Capelo, Jorge</style></author><author><style face="normal" font="default" size="100%">Phillips, Alan</style></author><author><style face="normal" font="default" size="100%">Tenreiro, Rogério</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cryphonectria naterciae: a new species in the Cryphonectria-Endothia complex and diagnostic molecular markers based on microsatellite-primed PCR.</style></title><secondary-title><style face="normal" font="default" size="100%">Fungal biology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Ascomycota</style></keyword><keyword><style  face="normal" font="default" size="100%">Ascomycota: classification</style></keyword><keyword><style  face="normal" font="default" size="100%">Ascomycota: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Ascomycota: isolation &amp; purification</style></keyword><keyword><style  face="normal" font="default" size="100%">Chestnut tree</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak tree</style></keyword><keyword><style  face="normal" font="default" size="100%">Cryphonectria parasitica</style></keyword><keyword><style  face="normal" font="default" size="100%">Cryphonectria radicalis (GACA)4</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA Primers</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA Primers: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Endothiella gyrosa</style></keyword><keyword><style  face="normal" font="default" size="100%">Fagaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">Fagaceae: microbiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungal</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungal Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungal Proteins: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungal: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Microsatellite Repeats</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular Sequence Data</style></keyword><keyword><style  face="normal" font="default" size="100%">MSP-PCR</style></keyword><keyword><style  face="normal" font="default" size="100%">Mycological Typing Techniques</style></keyword><keyword><style  face="normal" font="default" size="100%">Mycological Typing Techniques: methods</style></keyword><keyword><style  face="normal" font="default" size="100%">phylogeny</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Diseases</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Diseases: microbiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Polymerase Chain Reaction</style></keyword><keyword><style  face="normal" font="default" size="100%">Polymerase Chain Reaction: methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: microbiology</style></keyword><keyword><style  face="normal" font="default" size="100%">RFLP-PCR</style></keyword><keyword><style  face="normal" font="default" size="100%">Tubulin</style></keyword><keyword><style  face="normal" font="default" size="100%">Tubulin: genetics</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2011///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/21872182</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">115</style></volume><pages><style face="normal" font="default" size="100%">852 - 861</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">In a recent study intended to assess the distribution of Cryphonectria parasitica in Portugal, 22 morphologically atypical orange isolates were collected in the Midwestern regions. Eleven isolates were recovered from Castanea sativa, in areas severely affected by chestnut blight and eleven isolates from Quercus suber in areas with cork oak decline. These isolates were compared with known C. parasitica and Cryphonectria radicalis isolates using an integrated approach comprising morphological and molecular methods. Morphologically the atypical isolates were more similar to C. radicalis than to C. parasitica. Phylogenetic analyses based on internal transcribed spacer (ITS) and β-tubulin sequence data grouped the isolates in a well-supported clade separate from C. radicalis. Combining morphological, cultural, and molecular data Cryphonectria naterciae is newly described in the Cryphonectria-Endothia complex. Microsatellite-primed PCR fingerprinting with (GACA)(4) primer discriminated between C. naterciae, C. radicalis, and C. parasitica.</style></abstract><issue><style face="normal" font="default" size="100%">9</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;accession-num: 21872182</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Stone, G</style></author><author><style face="normal" font="default" size="100%">Atkinson, R</style></author><author><style face="normal" font="default" size="100%">Rokas, A</style></author><author><style face="normal" font="default" size="100%">Csoka, G</style></author><author><style face="normal" font="default" size="100%">Nieves-Aldrey, J L</style></author><author><style face="normal" font="default" size="100%">Csoka, G</style></author><author><style face="normal" font="default" size="100%">Nieves-Aldrey, J L</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Differential success in northwards range expansion between ecotypes of the marble gallwasp Andricus kollari: a tale of two lifecycles</style></title><secondary-title><style face="normal" font="default" size="100%">MOLECULAR ECOLOGY</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Alleles</style></keyword><keyword><style  face="normal" font="default" size="100%">Andricus</style></keyword><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Base Sequence</style></keyword><keyword><style  face="normal" font="default" size="100%">Cellulose Acetate</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytochrome b Group</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytochrome b Group: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytochrome b Group: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Electrophoresis</style></keyword><keyword><style  face="normal" font="default" size="100%">Environment</style></keyword><keyword><style  face="normal" font="default" size="100%">EUROPE</style></keyword><keyword><style  face="normal" font="default" size="100%">Evolution</style></keyword><keyword><style  face="normal" font="default" size="100%">Female</style></keyword><keyword><style  face="normal" font="default" size="100%">gallwasp</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic Variation</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic Variation: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">glacial refugia</style></keyword><keyword><style  face="normal" font="default" size="100%">host race</style></keyword><keyword><style  face="normal" font="default" size="100%">Hymenoptera</style></keyword><keyword><style  face="normal" font="default" size="100%">Hymenoptera: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Hymenoptera: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Hymenoptera: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">invasion</style></keyword><keyword><style  face="normal" font="default" size="100%">Life Cycle Stages</style></keyword><keyword><style  face="normal" font="default" size="100%">Mitochondrial</style></keyword><keyword><style  face="normal" font="default" size="100%">Mitochondrial: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Mitochondrial: isolation &amp; purification</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular Sequence Data</style></keyword><keyword><style  face="normal" font="default" size="100%">Parthenogenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">phylogeny</style></keyword><keyword><style  face="normal" font="default" size="100%">Polymerase Chain Reaction</style></keyword><keyword><style  face="normal" font="default" size="100%">range expansion</style></keyword><keyword><style  face="normal" font="default" size="100%">Sequence Alignment</style></keyword><keyword><style  face="normal" font="default" size="100%">Sequence Analysis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2001</style></year></dates><publisher><style face="normal" font="default" size="100%">WILEY-BLACKWELL</style></publisher><pub-location><style face="normal" font="default" size="100%">COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA</style></pub-location><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">761-778</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The Marble gallwasp Andricus kollari has a native range divided into two geographically separated lifecycles. In Eastern Europe and Turkey, the lifecycle involves a sexual generation on Turkey oak, Quercus cerris, while in Iberia and North Africa the sexual generation host is cork oak, Q. suber. Over the last 500 years, A. kollari has expanded its range into northern Europe, following human planting of Q. cem's from Italy and the Balkans. We ask: (i) what is the genetic relationship between eastern and western distributions of Andricus kollari? Can we determine which lifecycle is ancestral, and how long ago they diverged? (ii) To what extent have eastern and western native ranges contributed to northwards range expansion? (iii) Is there any evidence for hybridization between the two life cycle types? We present analyses of allozyme data for 13 polymorphic loci and of sequence variation for a 433 bp fragment of the mitochondrial cytochrome b gene. These show: (i) that four haplotype lineages (one in Spain, two in Hungary/Italy and one in Turkey) diverged more or less simultaneously between 1 and 2 million years ago, suggesting the existence of at least four refuges through recent ice age cycles. Our data cannot resolve which lifecycle type is ancestral. (ii) Populations north of putative refuges are divided into two sets. Populations in south-west France are allied to Spain, while ail remaining populations in northern Europe have been colonized from Italy and the Balkans. (iii) The transition from one race to another in south-west France is marked by abrupt transitions in the frequency of refuge-specific private alleles and corresponds closely to the northern limit of the distribution of cork oak. Although hybrids were detected in north-west France, none were detected where the two lifecycles meet in south-western France. The biology of oak gallwasps predicts that any hybrid zone will be narrow and limited to regions where Q. cem's and Q. suber meet. Our data suggest that eastern and western A. kollari are effectively separate species.</style></abstract><accession-num><style face="normal" font="default" size="100%">11298986</style></accession-num><notes><style face="normal" font="default" size="100%">From Duplicate 2 (Differential success in northwards range expansion between ecotypes of the marble gallwasp Andricus kollari: a tale of two lifecycles - Stone, G; Atkinson, R; Rokas, A; Csoka, G; Nieves-Aldrey, J L)</style></notes><research-notes><style face="normal" font="default" size="100%">From Duplicate 2 (Differential success in northwards range expansion between ecotypes of the marble gallwasp Andricus kollari: a tale of two lifecycles - Stone, G; Atkinson, R; Rokas, A; Csoka, G; Nieves-Aldrey, J L)</style></research-notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Stone, G.</style></author><author><style face="normal" font="default" size="100%">Atkinson, R.</style></author><author><style face="normal" font="default" size="100%">Rokas, A.</style></author><author><style face="normal" font="default" size="100%">Csoka, G.</style></author><author><style face="normal" font="default" size="100%">Nieves-Aldrey, J. L.</style></author><author><style face="normal" font="default" size="100%">Csoka, G.</style></author><author><style face="normal" font="default" size="100%">Nieves-Aldrey, J. L.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Differential success in northwards range expansion between ecotypes of the marble gallwasp Andricus kollari: a tale of two lifecycles</style></title><secondary-title><style face="normal" font="default" size="100%">MOLECULAR ECOLOGY</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Alleles</style></keyword><keyword><style  face="normal" font="default" size="100%">Andricus</style></keyword><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Base Sequence</style></keyword><keyword><style  face="normal" font="default" size="100%">Cellulose Acetate</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytochrome b Group</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytochrome b Group: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytochrome b Group: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Electrophoresis</style></keyword><keyword><style  face="normal" font="default" size="100%">Environment</style></keyword><keyword><style  face="normal" font="default" size="100%">EUROPE</style></keyword><keyword><style  face="normal" font="default" size="100%">Evolution</style></keyword><keyword><style  face="normal" font="default" size="100%">Female</style></keyword><keyword><style  face="normal" font="default" size="100%">gallwasp</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic Variation</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic Variation: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">glacial refugia</style></keyword><keyword><style  face="normal" font="default" size="100%">host race</style></keyword><keyword><style  face="normal" font="default" size="100%">Hymenoptera</style></keyword><keyword><style  face="normal" font="default" size="100%">Hymenoptera: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Hymenoptera: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Hymenoptera: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">invasion</style></keyword><keyword><style  face="normal" font="default" size="100%">Life Cycle Stages</style></keyword><keyword><style  face="normal" font="default" size="100%">Mitochondrial</style></keyword><keyword><style  face="normal" font="default" size="100%">Mitochondrial: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Mitochondrial: isolation &amp; purification</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular Sequence Data</style></keyword><keyword><style  face="normal" font="default" size="100%">Parthenogenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">phylogeny</style></keyword><keyword><style  face="normal" font="default" size="100%">Polymerase Chain Reaction</style></keyword><keyword><style  face="normal" font="default" size="100%">range expansion</style></keyword><keyword><style  face="normal" font="default" size="100%">Sequence Alignment</style></keyword><keyword><style  face="normal" font="default" size="100%">Sequence Analysis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2001</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2001///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/11298986</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">761 - 778</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The Marble gallwasp Andricus kollari has a native range divided into two geographically separated lifecycles. In Eastern Europe and Turkey, the lifecycle involves a sexual generation on Turkey oak, Quercus cerris, while in Iberia and North Africa the sexual generation host is cork oak, Q. suber. Over the last 500 years, A. kollari has expanded its range into northern Europe, following human planting of Q. cem's from Italy and the Balkans. We ask: (i) what is the genetic relationship between eastern and western distributions of Andricus kollari? Can we determine which lifecycle is ancestral, and how long ago they diverged? (ii) To what extent have eastern and western native ranges contributed to northwards range expansion? (iii) Is there any evidence for hybridization between the two life cycle types? We present analyses of allozyme data for 13 polymorphic loci and of sequence variation for a 433 bp fragment of the mitochondrial cytochrome b gene. These show: (i) that four haplotype lineages (one in Spain, two in Hungary/Italy and one in Turkey) diverged more or less simultaneously between 1 and 2 million years ago, suggesting the existence of at least four refuges through recent ice age cycles. Our data cannot resolve which lifecycle type is ancestral. (ii) Populations north of putative refuges are divided into two sets. Populations in south-west France are allied to Spain, while ail remaining populations in northern Europe have been colonized from Italy and the Balkans. (iii) The transition from one race to another in south-west France is marked by abrupt transitions in the frequency of refuge-specific private alleles and corresponds closely to the northern limit of the distribution of cork oak. Although hybrids were detected in north-west France, none were detected where the two lifecycles meet in south-western France. The biology of oak gallwasps predicts that any hybrid zone will be narrow and limited to regions where Q. cem's and Q. suber meet. Our data suggest that eastern and western A. kollari are effectively separate species.</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><notes><style face="normal" font="default" size="100%">From Duplicate 2 (Differential success in northwards range expansion between ecotypes of the marble gallwasp Andricus kollari: a tale of two lifecycles - Stone, G; Atkinson, R; Rokas, A; Csoka, G; Nieves-Aldrey, J L)From Duplicate 2 (Differential success in northwards range expansion between ecotypes of the marble gallwasp Andricus kollari: a tale of two lifecycles - Stone, G; Atkinson, R; Rokas, A; Csoka, G; Nieves-Aldrey, J L)The following values have no corresponding Zotero field:&lt;br/&gt;pub-location: COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA&lt;br/&gt;publisher: WILEY-BLACKWELL&lt;br/&gt;accession-num: 11298986</style></notes></record></records></xml>