<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Abril, Nieves</style></author><author><style face="normal" font="default" size="100%">Gion, Jean-Marc</style></author><author><style face="normal" font="default" size="100%">Kerner, René</style></author><author><style face="normal" font="default" size="100%">Müller-Starck, Gerhard</style></author><author><style face="normal" font="default" size="100%">Cerrillo, Rafael M. Navarro</style></author><author><style face="normal" font="default" size="100%">Plomion, Christophe</style></author><author><style face="normal" font="default" size="100%">Renaut, Jenny</style></author><author><style face="normal" font="default" size="100%">Valledor, Luis</style></author><author><style face="normal" font="default" size="100%">Jorrin-Novo, Jesús V.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Proteomics research on forest trees, the most recalcitrant and orphan plant species.</style></title><secondary-title><style face="normal" font="default" size="100%">Phytochemistry</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Forest tree proteomics</style></keyword><keyword><style  face="normal" font="default" size="100%">forest trees</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant proteomics</style></keyword><keyword><style  face="normal" font="default" size="100%">Woody plants</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2011///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/21353265</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">72</style></volume><pages><style face="normal" font="default" size="100%">1219 - 1242</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The contribution of proteomics to the knowledge of forest tree (the most recalcitrant and almost forgotten plant species) biology is being reviewed and discussed, based on the author's own research work and papers published up to November 2010. This review is organized in four introductory sections starting with the definition of forest trees (1), the description of the environmental and economic importance (2) and its derived current priorities and research lines for breeding and conservation (3) including forest tree genomics (4). These precede the main body of this review: a general overview to proteomics (5) for introducing the forest tree proteomics section (6). Proteomics, defined as scientific discipline or experimental approach, it will be discussed both from a conceptual and methodological point of view, commenting on realities, challenges and limitations. Proteomics research in woody plants is limited to a reduced number of genera, including Pinus, Picea, Populus, Eucalyptus, and Fagus, mainly using first-generation approaches, e.g., those based on two-dimensional electrophoresis coupled to mass spectrometry. This area joins the own limitations of the technique and the difficulty and recalcitrance of the plant species as an experimental system. Furthermore, it contributes to a deeper knowledge of some biological processes, namely growth, development, organogenesis, and responses to stresses, as it is also used in the characterization and cataloguing of natural populations and biodiversity (proteotyping) and in assisting breeding programmes.</style></abstract><issue><style face="normal" font="default" size="100%">10</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Elsevier Ltd&lt;br/&gt;accession-num: 21353265</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Bragança, Helena</style></author><author><style face="normal" font="default" size="100%">Tenreiro, Rogério</style></author><author><style face="normal" font="default" size="100%">Santos, Natércia</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Identification of Portuguese Armillaria Isolates by Classic Mating-Tests and RFLP-PCR Analysis of the ITS1 Region of Ribosomal DNA</style></title><secondary-title><style face="normal" font="default" size="100%">Silva Lusitana</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Armillaria mellea</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">rDNA</style></keyword><keyword><style  face="normal" font="default" size="100%">RFLP-PCR</style></keyword><keyword><style  face="normal" font="default" size="100%">Woody plants</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2004</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2004///</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">67 - 75</style></pages><isbn><style face="normal" font="default" size="100%">0870-6352 UL - http://www.scielo.gpeari.mctes.pt/scielo.php?script=sci_arttext&amp;pid=S0870-63522004000200006&amp;nrm=iso</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The diagnosis of Armillaria, a genus including distinct species of highly woody plantpathogenic root-infecting fungi with worldwide distribution, is usually based on morphological characteristics and mating-tests, although the PCR-based restriction fragment length polymorphism (RFLP-PCR), specifically in nuclear rDNA spacers, have also been applied. In the present study, mating-tests and restriction analysis of Internal Transcribed Spacer 1 (ITS1) were used to identify 20 isolates of a Portuguese Armillaria collection. Although the majority of the diploid isolates (80%) could be identified in diploid-haploid pairings, the method is laborious, takes too much time (up to 2 months), and presents a high rate of inconclusive results. The ITS1 region showed to be a reliable molecular marker for A. mellea, in particular when HinfI restriction analysis is applied, since two fragments with 245 bp and 125 bp have been obtained for this most aggressive species whereas 290 bp and 70 bp were produced from isolates of the other European species. As simple molecular techniques are involved and the whole procedure can be performed in one day, A. mellea identification by ITS1 analysis is a clearly accessible and more advantageous tool to plant pathology laboratories, mainly those involved on the control and preservation of forest trees</style></abstract><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: scielopt</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Bragança, Helena</style></author><author><style face="normal" font="default" size="100%">Tenreiro, Rogério</style></author><author><style face="normal" font="default" size="100%">Santos, Natércia</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Identification of Portuguese Armillaria Isolates by Classic Mating-Tests and RFLP-PCR Analysis of the ITS1 Region of Ribosomal DNA</style></title><secondary-title><style face="normal" font="default" size="100%">Silva Lusitana</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Armillaria mellea</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">rDNA</style></keyword><keyword><style  face="normal" font="default" size="100%">RFLP-PCR</style></keyword><keyword><style  face="normal" font="default" size="100%">Woody plants</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2004</style></year></dates><publisher><style face="normal" font="default" size="100%">scielopt</style></publisher><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">67-75</style></pages><isbn><style face="normal" font="default" size="100%">0870-6352 UL - http://www.scielo.gpeari.mctes.pt/scielo.php?script=sci_arttext&amp;pid=S0870-63522004000200006&amp;nrm=iso</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The diagnosis of Armillaria, a genus including distinct species of highly woody plantpathogenic root-infecting fungi with worldwide distribution, is usually based on morphological characteristics and mating-tests, although the PCR-based restriction fragment length polymorphism (RFLP-PCR), specifically in nuclear rDNA spacers, have also been applied. In the present study, mating-tests and restriction analysis of Internal Transcribed Spacer 1 (ITS1) were used to identify 20 isolates of a Portuguese Armillaria collection. Although the majority of the diploid isolates (80%) could be identified in diploid-haploid pairings, the method is laborious, takes too much time (up to 2 months), and presents a high rate of inconclusive results. The ITS1 region showed to be a reliable molecular marker for A. mellea, in particular when HinfI restriction analysis is applied, since two fragments with 245 bp and 125 bp have been obtained for this most aggressive species whereas 290 bp and 70 bp were produced from isolates of the other European species. As simple molecular techniques are involved and the whole procedure can be performed in one day, A. mellea identification by ITS1 analysis is a clearly accessible and more advantageous tool to plant pathology laboratories, mainly those involved on the control and preservation of forest trees</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Llusia, J</style></author><author><style face="normal" font="default" size="100%">Penuelas, J</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Changes in terpene content and emission in potted Mediterranean woody plants under severe drought</style></title><secondary-title><style face="normal" font="default" size="100%">Canadian Journal of Botany</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Drought</style></keyword><keyword><style  face="normal" font="default" size="100%">Mediterranean conditions</style></keyword><keyword><style  face="normal" font="default" size="100%">terpene concentration</style></keyword><keyword><style  face="normal" font="default" size="100%">Terpene emission</style></keyword><keyword><style  face="normal" font="default" size="100%">Woody plants</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1998</style></year></dates><publisher><style face="normal" font="default" size="100%">NRC Research Press</style></publisher><volume><style face="normal" font="default" size="100%">76</style></volume><pages><style face="normal" font="default" size="100%">1366-1373</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Terpene concentration and emission were studied in potted plants of some of the most common Mediterranean woody species (Pinus halepensis L., Pistacia lentiscus L., Cistus albidus L., Cistus monspeliensis L., Quercus ilex L., Quercus coccifera L., Phillyrea latifolia L., Phillyrea angustifolia L., and Arbutus unedo L.) under irrigation and under severe drought conditions that dropped relative water content to a range between 40% in Q. ilex and 85% in Phillyrea latifolia after withholding watering for one dry summer week. Terpene concentrations were detected in Pinus halepensis, Pistacia lentiscus, C. albidus, and C. monspeliensis, and they increased after withholding watering except in C. albidus. Terpene emission was detected in all species except Phillyrea angustifolia and A. unedo. Pinus halepensis showed the highest diurnal terpene emission rates of 86 µg·g-1 dry wt.·h-1 followed by C. albidus, Pistacia lentiscus, Q. ilex, Q. coccifera, Phillyrea latifolia, and C. monspeliensis (4 µg·g-1 dry wt.·h-1). Emitted terpenes represented from 0.33% of C fixed in C. monspeliensis to 10% in C. albidus. All species severely decreased their terpene emission rates under severe drought conditions. Emission by terpene-storing species (e.g., Pinus halepensis) was more related to temperature than in nonstoring species (e.g., Q. ilex), which showed emission relationships with photosynthetic rates. The monoterpenes alpha-pinene, beta-pinene, beta-phellandrene, and limonene and the sesquiterpene caryophyllene were the most abundant terpenes stored and emitted by these Mediterranean plant species.Key words: drought, Mediterranean conditions, terpene concentration, terpene emission, woody plants</style></abstract><notes><style face="normal" font="default" size="100%">doi: 10.1139/b98-141</style></notes><research-notes><style face="normal" font="default" size="100%">doi: 10.1139/b98-141</style></research-notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Llusia, J.</style></author><author><style face="normal" font="default" size="100%">Penuelas, J.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Changes in terpene content and emission in potted Mediterranean woody plants under severe drought</style></title><secondary-title><style face="normal" font="default" size="100%">Canadian Journal of Botany</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Drought</style></keyword><keyword><style  face="normal" font="default" size="100%">Mediterranean conditions</style></keyword><keyword><style  face="normal" font="default" size="100%">terpene concentration</style></keyword><keyword><style  face="normal" font="default" size="100%">Terpene emission</style></keyword><keyword><style  face="normal" font="default" size="100%">Woody plants</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1998</style></year><pub-dates><date><style  face="normal" font="default" size="100%">1998///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1139/b98-141</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">76</style></volume><pages><style face="normal" font="default" size="100%">1366 - 1373</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Terpene concentration and emission were studied in potted plants of some of the most common Mediterranean woody species (Pinus halepensis L., Pistacia lentiscus L., Cistus albidus L., Cistus monspeliensis L., Quercus ilex L., Quercus coccifera L., Phillyrea latifolia L., Phillyrea angustifolia L., and Arbutus unedo L.) under irrigation and under severe drought conditions that dropped relative water content to a range between 40% in Q. ilex and 85% in Phillyrea latifolia after withholding watering for one dry summer week. Terpene concentrations were detected in Pinus halepensis, Pistacia lentiscus, C. albidus, and C. monspeliensis, and they increased after withholding watering except in C. albidus. Terpene emission was detected in all species except Phillyrea angustifolia and A. unedo. Pinus halepensis showed the highest diurnal terpene emission rates of 86 µg·g-1 dry wt.·h-1 followed by C. albidus, Pistacia lentiscus, Q. ilex, Q. coccifera, Phillyrea latifolia, and C. monspeliensis (4 µg·g-1 dry wt.·h-1). Emitted terpenes represented from 0.33% of C fixed in C. monspeliensis to 10% in C. albidus. All species severely decreased their terpene emission rates under severe drought conditions. Emission by terpene-storing species (e.g., Pinus halepensis) was more related to temperature than in nonstoring species (e.g., Q. ilex), which showed emission relationships with photosynthetic rates. The monoterpenes alpha-pinene, beta-pinene, beta-phellandrene, and limonene and the sesquiterpene caryophyllene were the most abundant terpenes stored and emitted by these Mediterranean plant species.Key words: drought, Mediterranean conditions, terpene concentration, terpene emission, woody plants</style></abstract><issue><style face="normal" font="default" size="100%">8</style></issue><notes><style face="normal" font="default" size="100%">doi: 10.1139/b98-141doi: 10.1139/b98-141The following values have no corresponding Zotero field:&lt;br/&gt;publisher: NRC Research Press</style></notes></record></records></xml>