<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>5</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Álvarez-Fernández, Rubén</style></author><author><style face="normal" font="default" size="100%">Ordás, Ricardo-Javier</style></author></authors><secondary-authors><author><style face="normal" font="default" size="100%">Loyola-Vargas, Víctor M.</style></author><author><style face="normal" font="default" size="100%">Ochoa-Alejo, Neftalí</style></author></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Improved Genetic Transformation of Cork Oak (Quercus suber L.)</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Cell Culture Protocols SE - 28</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">AGL1</style></keyword><keyword><style  face="normal" font="default" size="100%">Agrobacterium tumefaciens</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Fagaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">Herbicide Resistance</style></keyword><keyword><style  face="normal" font="default" size="100%">kanamycin resistance</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">Somatic embryogenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Tree genetic transformation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2012///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1007/978-1-61779-818-4_28</style></url></web-urls></urls><publisher><style face="normal" font="default" size="100%">Humana Press</style></publisher><volume><style face="normal" font="default" size="100%">877</style></volume><pages><style face="normal" font="default" size="100%">385 - 399 LA -- English</style></pages><isbn><style face="normal" font="default" size="100%">978-1-61779-817-7</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">An Agrobacterium-mediated transformation system for selected mature Quercus suber L. trees has been established. Leaf-derived somatic embryos in an early stage of development are inoculated with an AGL1 strain harboring a kanamycin-selectable plasmid carrying the gene of interest. The transformed embryos are induced to germinate and the plantlets transferred to soil. This protocol, from adult cork oak to transformed plantlet, can be completed in about one and a half years. Transformation efficiencies (i.e., percentage of inoculated explants that yield independent transgenic embryogenic lines) vary depending on the cork oak genotype, reaching up to 43%.</style></abstract><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;periodical: Plant Cell Culture Protocols SE - 28&lt;br/&gt;electronic-resource-num: 10.1007/978-1-61779-818-4_28</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Alvarez, Rubén</style></author><author><style face="normal" font="default" size="100%">Alvarez, José M</style></author><author><style face="normal" font="default" size="100%">Humara, Jaime M</style></author><author><style face="normal" font="default" size="100%">Revilla, Angeles</style></author><author><style face="normal" font="default" size="100%">Ordás, Ricardo J</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Genetic transformation of cork oak (Quercus suber L.) for herbicide resistance.</style></title><secondary-title><style face="normal" font="default" size="100%">Biotechnology letters</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Acetyltransferases</style></keyword><keyword><style  face="normal" font="default" size="100%">Acetyltransferases: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Acetyltransferases: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene Dosage</style></keyword><keyword><style  face="normal" font="default" size="100%">Genomic Instability</style></keyword><keyword><style  face="normal" font="default" size="100%">Herbicide Resistance</style></keyword><keyword><style  face="normal" font="default" size="100%">Herbicides</style></keyword><keyword><style  face="normal" font="default" size="100%">Herbicides: toxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plants, Genetically Modified</style></keyword><keyword><style  face="normal" font="default" size="100%">Plants, Genetically Modified: drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Plants, Genetically Modified: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Plants, Genetically Modified: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Plasmids</style></keyword><keyword><style  face="normal" font="default" size="100%">Promoter Regions, Genetic</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Rhizobium</style></keyword><keyword><style  face="normal" font="default" size="100%">Rhizobium: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Transformation, Genetic</style></keyword><keyword><style  face="normal" font="default" size="100%">Zea mays</style></keyword><keyword><style  face="normal" font="default" size="100%">Zea mays: genetics</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2009</style></year></dates><volume><style face="normal" font="default" size="100%">31</style></volume><pages><style face="normal" font="default" size="100%">1477-83</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The bar gene was introduced into the cork oak genome. Cork oak embryogenic masses were transformed using the Agrobacterium strain AGL1 which carried the plasmid pBINUbiBar. This vector harbours the genes, nptII and bar, the latter under control of the maize ubiquitin promoter. The transgenic embryogenic lines were cryopreserved. Varying activities of phosphinothricin acetyl transferase were detected among the lines, which carried 1-4 copies of the insert. Molecular and biochemical assays confirmed the stability and expression of the transgenes 3 months after thawing the cultures. These results demonstrate genetic engineering of herbicide tolerance in Quercus spp.</style></abstract><accession-num><style face="normal" font="default" size="100%">19543858</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Alvarez, Rubén</style></author><author><style face="normal" font="default" size="100%">Alvarez, José M.</style></author><author><style face="normal" font="default" size="100%">Humara, Jaime M.</style></author><author><style face="normal" font="default" size="100%">Revilla, Angeles</style></author><author><style face="normal" font="default" size="100%">Ordás, Ricardo J.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Genetic transformation of cork oak (Quercus suber L.) for herbicide resistance.</style></title><secondary-title><style face="normal" font="default" size="100%">Biotechnology letters</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Acetyltransferases</style></keyword><keyword><style  face="normal" font="default" size="100%">Acetyltransferases: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Acetyltransferases: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene Dosage</style></keyword><keyword><style  face="normal" font="default" size="100%">Genomic Instability</style></keyword><keyword><style  face="normal" font="default" size="100%">Herbicide Resistance</style></keyword><keyword><style  face="normal" font="default" size="100%">Herbicides</style></keyword><keyword><style  face="normal" font="default" size="100%">Herbicides: toxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plants, Genetically Modified</style></keyword><keyword><style  face="normal" font="default" size="100%">Plants, Genetically Modified: drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Plants, Genetically Modified: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Plants, Genetically Modified: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Plasmids</style></keyword><keyword><style  face="normal" font="default" size="100%">Promoter Regions, Genetic</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Rhizobium</style></keyword><keyword><style  face="normal" font="default" size="100%">Rhizobium: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Transformation, Genetic</style></keyword><keyword><style  face="normal" font="default" size="100%">Zea mays</style></keyword><keyword><style  face="normal" font="default" size="100%">Zea mays: genetics</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2009</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2009///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/19543858</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">31</style></volume><pages><style face="normal" font="default" size="100%">1477 - 83</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The bar gene was introduced into the cork oak genome. Cork oak embryogenic masses were transformed using the Agrobacterium strain AGL1 which carried the plasmid pBINUbiBar. This vector harbours the genes, nptII and bar, the latter under control of the maize ubiquitin promoter. The transgenic embryogenic lines were cryopreserved. Varying activities of phosphinothricin acetyl transferase were detected among the lines, which carried 1-4 copies of the insert. Molecular and biochemical assays confirmed the stability and expression of the transgenes 3 months after thawing the cultures. These results demonstrate genetic engineering of herbicide tolerance in Quercus spp.</style></abstract><issue><style face="normal" font="default" size="100%">9</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;accession-num: 19543858</style></notes></record></records></xml>