<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>5</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Álvarez-Fernández, Rubén</style></author><author><style face="normal" font="default" size="100%">Ordás, Ricardo-Javier</style></author></authors><secondary-authors><author><style face="normal" font="default" size="100%">Loyola-Vargas, Víctor M.</style></author><author><style face="normal" font="default" size="100%">Ochoa-Alejo, Neftalí</style></author></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Improved Genetic Transformation of Cork Oak (Quercus suber L.)</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Cell Culture Protocols SE - 28</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">AGL1</style></keyword><keyword><style  face="normal" font="default" size="100%">Agrobacterium tumefaciens</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Fagaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">Herbicide Resistance</style></keyword><keyword><style  face="normal" font="default" size="100%">kanamycin resistance</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">Somatic embryogenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Tree genetic transformation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2012///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1007/978-1-61779-818-4_28</style></url></web-urls></urls><publisher><style face="normal" font="default" size="100%">Humana Press</style></publisher><volume><style face="normal" font="default" size="100%">877</style></volume><pages><style face="normal" font="default" size="100%">385 - 399 LA -- English</style></pages><isbn><style face="normal" font="default" size="100%">978-1-61779-817-7</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">An Agrobacterium-mediated transformation system for selected mature Quercus suber L. trees has been established. Leaf-derived somatic embryos in an early stage of development are inoculated with an AGL1 strain harboring a kanamycin-selectable plasmid carrying the gene of interest. The transformed embryos are induced to germinate and the plantlets transferred to soil. This protocol, from adult cork oak to transformed plantlet, can be completed in about one and a half years. Transformation efficiencies (i.e., percentage of inoculated explants that yield independent transgenic embryogenic lines) vary depending on the cork oak genotype, reaching up to 43%.</style></abstract><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;periodical: Plant Cell Culture Protocols SE - 28&lt;br/&gt;electronic-resource-num: 10.1007/978-1-61779-818-4_28</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>7</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Alvarez, Rubén</style></author><author><style face="normal" font="default" size="100%">Toribio, Mariano</style></author><author><style face="normal" font="default" size="100%">Cortizo, Millán</style></author><author><style face="normal" font="default" size="100%">Ordás Fernández, Ricardo-Javier</style></author></authors><secondary-authors><author><style face="normal" font="default" size="100%">Wang, K</style></author></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Cork Oak Trees (Quercus suber L.).</style></title><secondary-title><style face="normal" font="default" size="100%">Methods in molecular biology (Clifton, N.J.)</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">AGL1</style></keyword><keyword><style  face="normal" font="default" size="100%">Agrobacterium tumefaciens</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Fagaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">kanamycin resistance</style></keyword><keyword><style  face="normal" font="default" size="100%">pBINUbiGUSint</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">Somatic embryogenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Tree genetic transformation</style></keyword><keyword><style  face="normal" font="default" size="100%">β-glucuronidase</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2006</style></year></dates><publisher><style face="normal" font="default" size="100%">Humana Press</style></publisher><pub-location><style face="normal" font="default" size="100%">Totowa</style></pub-location><volume><style face="normal" font="default" size="100%">344</style></volume><pages><style face="normal" font="default" size="100%">113-123</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">A transformation system for selected mature Quercus suber L. trees using Agrobacterium tumefaciens has been established. Embryos obtained from recurrent proliferating embryogenic masses are inoculated with AGL1 strain harbouring the plasmid pBINUbiGUSint, which carries the nptII and uidA genes. Evidence of stable transgene integration is obtained by polymerase chain reaction for nptII and uidA genes, Southern blotting and expression of the uidA gene. The transgenic embryos are germinated and successfully transferred to soil.</style></abstract><accession-num><style face="normal" font="default" size="100%">17033056</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>5</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Alvarez, Rubén</style></author><author><style face="normal" font="default" size="100%">Toribio, Mariano</style></author><author><style face="normal" font="default" size="100%">Cortizo, Millán</style></author><author><style face="normal" font="default" size="100%">Ordás Fernández, Ricardo-Javier</style></author></authors><secondary-authors><author><style face="normal" font="default" size="100%">Wang, K.</style></author></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Cork Oak Trees (Quercus suber L.).</style></title><secondary-title><style face="normal" font="default" size="100%">Methods in molecular biology (Clifton, N.J.)</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">AGL1</style></keyword><keyword><style  face="normal" font="default" size="100%">Agrobacterium tumefaciens</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Fagaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">kanamycin resistance</style></keyword><keyword><style  face="normal" font="default" size="100%">pBINUbiGUSint</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">Somatic embryogenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Tree genetic transformation</style></keyword><keyword><style  face="normal" font="default" size="100%">β-glucuronidase</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2006</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2006///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/17033056</style></url></web-urls></urls><publisher><style face="normal" font="default" size="100%">Humana Press</style></publisher><pub-location><style face="normal" font="default" size="100%">Totowa</style></pub-location><volume><style face="normal" font="default" size="100%">344</style></volume><pages><style face="normal" font="default" size="100%">113 - 123</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">A transformation system for selected mature Quercus suber L. trees using Agrobacterium tumefaciens has been established. Embryos obtained from recurrent proliferating embryogenic masses are inoculated with AGL1 strain harbouring the plasmid pBINUbiGUSint, which carries the nptII and uidA genes. Evidence of stable transgene integration is obtained by polymerase chain reaction for nptII and uidA genes, Southern blotting and expression of the uidA gene. The transgenic embryos are germinated and successfully transferred to soil.</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;periodical: Methods in molecular biology (Clifton, N.J.)&lt;br/&gt;accession-num: 17033056&lt;br/&gt;electronic-resource-num: 10.1385/1-59745-131-2:113</style></notes></record></records></xml>