<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Arenal, Francisco</style></author><author><style face="normal" font="default" size="100%">Platas, Gonzalo</style></author><author><style face="normal" font="default" size="100%">Pelaez, Fernando</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A new endophytic species of Preussia (Sporormiaceae) inferred from morphological observations and molecular phylogenetic analysis</style></title><secondary-title><style face="normal" font="default" size="100%">FUNGAL DIVERSITY</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Ascomycota</style></keyword><keyword><style  face="normal" font="default" size="100%">Bayesian analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">elongation factor</style></keyword><keyword><style  face="normal" font="default" size="100%">ITS</style></keyword><keyword><style  face="normal" font="default" size="100%">LSU</style></keyword><keyword><style  face="normal" font="default" size="100%">maximum likelihood</style></keyword><keyword><style  face="normal" font="default" size="100%">phylogeny</style></keyword><keyword><style  face="normal" font="default" size="100%">rDNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Spain</style></keyword><keyword><style  face="normal" font="default" size="100%">Sporormiaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">taxonomy</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2007</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2007///</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">25</style></volume><pages><style face="normal" font="default" size="100%">1 - 17</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">A new species of Preussia as an endophyte of different typical Mediterranean plant species is newly described and illustrated based on molecular and morphological features. Preussia mediterranea resembles P. australis, P. africana and P. similis from a morphological point of view, but appeared closer to P. minima and P. isabellae by molecular analysis. Phylogenetic analysis of the entire ITS region combined with the D1-D2 domains of the 28S rRNA gene, and a fragment of the elongation factor EF-1 alpha gene, using Maximum Likelihood and a Bayesian Markov Chain Monte Carlo approach, generally resulted in statistically well-supported clades for all the species studied. Morphological data and molecular phylogenetic analysis supported the recognition of the new taxon.</style></abstract><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;pub-location: NORTHEAST FORESTRY UNIV, HARBIN, 00000, PEOPLES R CHINA&lt;br/&gt;publisher: FUNGAL DIVERSITY PRESS</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Alves, Artur</style></author><author><style face="normal" font="default" size="100%">Phillips, Alan J. L.</style></author><author><style face="normal" font="default" size="100%">Henriques, Isabel</style></author><author><style face="normal" font="default" size="100%">Correia, António</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Evaluation of amplified ribosomal DNA restriction analysis as a method for the identification of Botryosphaeria species.</style></title><secondary-title><style face="normal" font="default" size="100%">FEMS microbiology letters</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">ARDRA</style></keyword><keyword><style  face="normal" font="default" size="100%">Botryosphaeria</style></keyword><keyword><style  face="normal" font="default" size="100%">D1/D2 domains</style></keyword><keyword><style  face="normal" font="default" size="100%">ITS</style></keyword><keyword><style  face="normal" font="default" size="100%">rDNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Species identification</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2005</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2005///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/15837376</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">245</style></volume><pages><style face="normal" font="default" size="100%">221 - 229</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The polymerase chain reaction was used to amplify a rDNA fragment containing the internal transcribed spacers (ITS1-5.8S-ITS2) and the D1/D2 variable domains of the 28S rDNA from 10 species of the genus Botryosphaeria (Fungi, Ascomycota). Restriction analysis of the amplicons with frequent-cutting endonucleases (amplified ribosomal DNA restriction analysis) allowed the definition of 12 rDNA haplotypes. Each of the rDNA haplotypes could be unambiguously assigned to a single Botryosphaeria species, thus allowing clear identification of all the species tested. Intraspecific polymorphism was very low and detected only in Botryosphaeria parva and Botryosphaeria dothidea. Cluster analysis of banding patterns of the isolates corresponded well with known species delineations. The method described in this paper provides a simple and rapid procedure for the differentiation and identification of Botryosphaeria isolates at the species level.</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;accession-num: 15837376</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Bragança, Helena</style></author><author><style face="normal" font="default" size="100%">Tenreiro, Rogério</style></author><author><style face="normal" font="default" size="100%">Santos, Natércia</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Identification of Portuguese Armillaria Isolates by Classic Mating-Tests and RFLP-PCR Analysis of the ITS1 Region of Ribosomal DNA</style></title><secondary-title><style face="normal" font="default" size="100%">Silva Lusitana</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Armillaria mellea</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">rDNA</style></keyword><keyword><style  face="normal" font="default" size="100%">RFLP-PCR</style></keyword><keyword><style  face="normal" font="default" size="100%">Woody plants</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2004</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2004///</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">67 - 75</style></pages><isbn><style face="normal" font="default" size="100%">0870-6352 UL - http://www.scielo.gpeari.mctes.pt/scielo.php?script=sci_arttext&amp;pid=S0870-63522004000200006&amp;nrm=iso</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The diagnosis of Armillaria, a genus including distinct species of highly woody plantpathogenic root-infecting fungi with worldwide distribution, is usually based on morphological characteristics and mating-tests, although the PCR-based restriction fragment length polymorphism (RFLP-PCR), specifically in nuclear rDNA spacers, have also been applied. In the present study, mating-tests and restriction analysis of Internal Transcribed Spacer 1 (ITS1) were used to identify 20 isolates of a Portuguese Armillaria collection. Although the majority of the diploid isolates (80%) could be identified in diploid-haploid pairings, the method is laborious, takes too much time (up to 2 months), and presents a high rate of inconclusive results. The ITS1 region showed to be a reliable molecular marker for A. mellea, in particular when HinfI restriction analysis is applied, since two fragments with 245 bp and 125 bp have been obtained for this most aggressive species whereas 290 bp and 70 bp were produced from isolates of the other European species. As simple molecular techniques are involved and the whole procedure can be performed in one day, A. mellea identification by ITS1 analysis is a clearly accessible and more advantageous tool to plant pathology laboratories, mainly those involved on the control and preservation of forest trees</style></abstract><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: scielopt</style></notes></record></records></xml>