<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Barrico, Lurdes</style></author><author><style face="normal" font="default" size="100%">Rodríguez-Echeverría, Susana</style></author><author><style face="normal" font="default" size="100%">Freitas, Helena</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Diversity of soil basidiomycete communities associated with Quercus suber L. in Portuguese montados</style></title><secondary-title><style face="normal" font="default" size="100%">European Journal of Soil Biology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Basidiomycete</style></keyword><keyword><style  face="normal" font="default" size="100%">Dehesa</style></keyword><keyword><style  face="normal" font="default" size="100%">dgge</style></keyword><keyword><style  face="normal" font="default" size="100%">diversity</style></keyword><keyword><style  face="normal" font="default" size="100%">montado</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2010</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2010///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://linkinghub.elsevier.com/retrieve/pii/S1164556310000294</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">46</style></volume><pages><style face="normal" font="default" size="100%">280 - 287</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The montado is an agro-silvo pastoral system characterized by an open oak formation combined with shrubs or a rotation of cultures/pastures/fallow. A key, but frequently overlooked, component of these systems is the fungal community associated with the dominant oaks. We present a study of the basidiomycete community in montado areas of Quercus suber L. from Central-Western Portugal, based on fruiting body assessment and PCR-DGGE of soil mycelium. Soil available phosphorus and organic matter content were also determined. The most frequent fruiting body found in the cork oak montados belonged to Lactarius, Clitocybe, Russula and Cortinarius species. Lactarius chrysorrheus was the most widely distributed species. Soil management practices cause a temporal increase in soil available phosphorus, and harrowing and fertilizer application also result in an increase in soil organic matter content. Mechanical clearing with recent soil disturbance had a negative effect on the richness and diversity of the basidiomycete community assessed using fruiting body. The molecular analysis of the basidiomycete community separated the sites with recent shrub-removal from the others sites. Our results show that soil management techniques that avoid disturbance of the top-soil layers are the best way to preserve the structure and diversity of the soil fungal community in the montado.</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Elsevier Masson SAS</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Dedeyan, B.</style></author><author><style face="normal" font="default" size="100%">Klonowska, A.</style></author><author><style face="normal" font="default" size="100%">Tagger, S.</style></author><author><style face="normal" font="default" size="100%">Tron, T.</style></author><author><style face="normal" font="default" size="100%">Iacazio, G.</style></author><author><style face="normal" font="default" size="100%">Gil, G.</style></author><author><style face="normal" font="default" size="100%">Le petit, J.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Biochemical and molecular characterization of a laccase from Marasmius quercophilus</style></title><secondary-title><style face="normal" font="default" size="100%">APPLIED AND ENVIRONMENTAL MICROBIOLOGY</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Basidiomycete</style></keyword><keyword><style  face="normal" font="default" size="100%">litter</style></keyword><keyword><style  face="normal" font="default" size="100%">Marasmius quercophilus</style></keyword><keyword><style  face="normal" font="default" size="100%">mediterranean forest</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex L.</style></keyword><keyword><style  face="normal" font="default" size="100%">structural genes</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2000</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2000///</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">66</style></volume><pages><style face="normal" font="default" size="100%">925 - 929</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The basidiomycete Marasmius quercophilus is commonly found during autumn on the decaying litter of the evergreen oak (Quercus ilex L.), a plant characteristic of Mediterranean forest. This white-rot fungus colonizes the leaf surface with rhizomorphs, causing a total bleaching of the leaf. In synthetic liquid media, this white-rot fungus has strong laccase activity. From a three-step chromatographic procedure, we purified a major isoform to homogeneity, The gene encodes a monomeric glycoprotein of approximately 63 kDa, with a 3.6 isoelectric point, that contains 12% carbohydrate, Spectroscopic analysis of the purified enzyme (UV/visible and electron paramagnetic resonance, atomic absorption) confirmed that it belongs to the ``blue copper oxidase{''} family. With syringaldazine as the substrate, the enzyme's pH optimum was 4.5, the optimal temperature was 75 degrees C, and the K-m was 7.1 mu M. The structural gene, lac1, was cloned and sequenced. This gene encodes a 517-amino-acid protein 99% identical to a laccase produced by PM1, an unidentified basidiomycete previously isolated from wastewater from a paper factory in Spain. This similarity may be explained by the ecological distribution of the evergreen oak in Mediterranean forest.</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><notes><style face="normal" font="default" size="100%">Keys: APSKeys: APSThe following values have no corresponding Zotero field:&lt;br/&gt;pub-location: 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 USA&lt;br/&gt;publisher: AMER SOC MICROBIOLOGY</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Dedeyan, B</style></author><author><style face="normal" font="default" size="100%">Klonowska, A</style></author><author><style face="normal" font="default" size="100%">Tagger, S</style></author><author><style face="normal" font="default" size="100%">Tron, T</style></author><author><style face="normal" font="default" size="100%">Iacazio, G</style></author><author><style face="normal" font="default" size="100%">Gil, G</style></author><author><style face="normal" font="default" size="100%">Le petit, J</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Biochemical and molecular characterization of a laccase from Marasmius quercophilus</style></title><secondary-title><style face="normal" font="default" size="100%">APPLIED AND ENVIRONMENTAL MICROBIOLOGY</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Basidiomycete</style></keyword><keyword><style  face="normal" font="default" size="100%">litter</style></keyword><keyword><style  face="normal" font="default" size="100%">Marasmius quercophilus</style></keyword><keyword><style  face="normal" font="default" size="100%">mediterranean forest</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex L.</style></keyword><keyword><style  face="normal" font="default" size="100%">structural genes</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2000</style></year></dates><publisher><style face="normal" font="default" size="100%">AMER SOC MICROBIOLOGY</style></publisher><pub-location><style face="normal" font="default" size="100%">1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 USA</style></pub-location><volume><style face="normal" font="default" size="100%">66</style></volume><pages><style face="normal" font="default" size="100%">925-929</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The basidiomycete Marasmius quercophilus is commonly found during autumn on the decaying litter of the evergreen oak (Quercus ilex L.), a plant characteristic of Mediterranean forest. This white-rot fungus colonizes the leaf surface with rhizomorphs, causing a total bleaching of the leaf. In synthetic liquid media, this white-rot fungus has strong laccase activity. From a three-step chromatographic procedure, we purified a major isoform to homogeneity, The gene encodes a monomeric glycoprotein of approximately 63 kDa, with a 3.6 isoelectric point, that contains 12% carbohydrate, Spectroscopic analysis of the purified enzyme (UV/visible and electron paramagnetic resonance, atomic absorption) confirmed that it belongs to the ``blue copper oxidase{''} family. With syringaldazine as the substrate, the enzyme's pH optimum was 4.5, the optimal temperature was 75 degrees C, and the K-m was 7.1 mu M. The structural gene, lac1, was cloned and sequenced. This gene encodes a 517-amino-acid protein 99% identical to a laccase produced by PM1, an unidentified basidiomycete previously isolated from wastewater from a paper factory in Spain. This similarity may be explained by the ecological distribution of the evergreen oak in Mediterranean forest.</style></abstract><notes><style face="normal" font="default" size="100%">Keys: APS</style></notes><research-notes><style face="normal" font="default" size="100%">Keys: APS</style></research-notes></record></records></xml>