<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Bragança, Helena</style></author><author><style face="normal" font="default" size="100%">Rigling, Daniel</style></author><author><style face="normal" font="default" size="100%">Diogo, Eugénio</style></author><author><style face="normal" font="default" size="100%">Capelo, Jorge</style></author><author><style face="normal" font="default" size="100%">Phillips, Alan</style></author><author><style face="normal" font="default" size="100%">Tenreiro, Rogério</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cryphonectria naterciae: a new species in the Cryphonectria-Endothia complex and diagnostic molecular markers based on microsatellite-primed PCR.</style></title><secondary-title><style face="normal" font="default" size="100%">Fungal biology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Ascomycota</style></keyword><keyword><style  face="normal" font="default" size="100%">Ascomycota: classification</style></keyword><keyword><style  face="normal" font="default" size="100%">Ascomycota: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Ascomycota: isolation &amp; purification</style></keyword><keyword><style  face="normal" font="default" size="100%">Chestnut tree</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak tree</style></keyword><keyword><style  face="normal" font="default" size="100%">Cryphonectria parasitica</style></keyword><keyword><style  face="normal" font="default" size="100%">Cryphonectria radicalis (GACA)4</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA Primers</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA Primers: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Endothiella gyrosa</style></keyword><keyword><style  face="normal" font="default" size="100%">Fagaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">Fagaceae: microbiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungal</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungal Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungal Proteins: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungal: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Microsatellite Repeats</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular Sequence Data</style></keyword><keyword><style  face="normal" font="default" size="100%">MSP-PCR</style></keyword><keyword><style  face="normal" font="default" size="100%">Mycological Typing Techniques</style></keyword><keyword><style  face="normal" font="default" size="100%">Mycological Typing Techniques: methods</style></keyword><keyword><style  face="normal" font="default" size="100%">phylogeny</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Diseases</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Diseases: microbiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Polymerase Chain Reaction</style></keyword><keyword><style  face="normal" font="default" size="100%">Polymerase Chain Reaction: methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: microbiology</style></keyword><keyword><style  face="normal" font="default" size="100%">RFLP-PCR</style></keyword><keyword><style  face="normal" font="default" size="100%">Tubulin</style></keyword><keyword><style  face="normal" font="default" size="100%">Tubulin: genetics</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2011///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/21872182</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">115</style></volume><pages><style face="normal" font="default" size="100%">852 - 861</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">In a recent study intended to assess the distribution of Cryphonectria parasitica in Portugal, 22 morphologically atypical orange isolates were collected in the Midwestern regions. Eleven isolates were recovered from Castanea sativa, in areas severely affected by chestnut blight and eleven isolates from Quercus suber in areas with cork oak decline. These isolates were compared with known C. parasitica and Cryphonectria radicalis isolates using an integrated approach comprising morphological and molecular methods. Morphologically the atypical isolates were more similar to C. radicalis than to C. parasitica. Phylogenetic analyses based on internal transcribed spacer (ITS) and β-tubulin sequence data grouped the isolates in a well-supported clade separate from C. radicalis. Combining morphological, cultural, and molecular data Cryphonectria naterciae is newly described in the Cryphonectria-Endothia complex. Microsatellite-primed PCR fingerprinting with (GACA)(4) primer discriminated between C. naterciae, C. radicalis, and C. parasitica.</style></abstract><issue><style face="normal" font="default" size="100%">9</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;accession-num: 21872182</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Bergero, R.</style></author><author><style face="normal" font="default" size="100%">Perotto, S.</style></author><author><style face="normal" font="default" size="100%">Girlanda, M.</style></author><author><style face="normal" font="default" size="100%">Vidano, G.</style></author><author><style face="normal" font="default" size="100%">Luppi, a M.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Ericoid mycorrhizal fungi are common root associates of a Mediterranean ectomycorrhizal plant (Quercus ilex)</style></title><secondary-title><style face="normal" font="default" size="100%">Molecular Ecology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">ectomycorrhizas</style></keyword><keyword><style  face="normal" font="default" size="100%">Erica arborea</style></keyword><keyword><style  face="normal" font="default" size="100%">ericoid fungi</style></keyword><keyword><style  face="normal" font="default" size="100%">fungi</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungi: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungi: isolation &amp; purification</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungi: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic</style></keyword><keyword><style  face="normal" font="default" size="100%">Mediterranean ecosystem</style></keyword><keyword><style  face="normal" font="default" size="100%">Mediterranean Region</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular Sequence Data</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Diseases</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Diseases: microbiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Roots</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Roots: microbiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Polymorphism</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">Restriction Fragment Length</style></keyword><keyword><style  face="normal" font="default" size="100%">Ribosomal Spacer</style></keyword><keyword><style  face="normal" font="default" size="100%">Trees</style></keyword><keyword><style  face="normal" font="default" size="100%">Trees: microbiology</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2000</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2000///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/11050558http://dx.doi.org/10.1046/j.1365-294x.2000.01059.x</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">1639 - 1649</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Mycorrhiza samples of neighbouring Quercus ilex and Erica arborea plants collected in a postcutting habitat were processed to see whether plants differing in mycorrhizal status harbour the same root endophytes. Three experiments were performed in parallel: (i) isolation, identification and molecular characterization of fungi from surface-sterilized roots of both plant species; (ii) re-inoculation of fungal isolates on axenic E. arborea and Q. ilex seedlings; (iii) direct inoculation of field-collected Q. ilex ectomycorrhizas onto E. arborea seedlings. About 70 and 150 fungal isolates were obtained from roots of Q. ilex and E. arborea, respectively. Among them, Oidiodendron species and five cultural morphotypes of sterile isolates formed typical ericoid mycorrhizas on E. arborea in vitro. Fungi with such mycorrhizal ability were derived from both host plants. Isolates belonging to one of these morphotypes (sd9) also exhibited an unusual pattern of colonization, with an additional extracellular hyphal net. Ericoid mycorrhizas were also readily obtained by direct inoculation of E. arborea seedlings with Q. ilex ectomycorrhizal tips. Polymerase chain–restriction fragment length polymorphism and random amplified polymorphic DNA analyses of the shared sterile morphotypes demonstrate, in the case of sd9, the occurrence of the same genet on the two host plants. These results indicate that ericoid mycorrhizal fungi associate with ectomycorrhizal roots, and the ecological significance of this finding is discussed.</style></abstract><issue><style face="normal" font="default" size="100%">10</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Blackwell Science Ltd&lt;br/&gt;accession-num: 11050558</style></notes></record></records></xml>