<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Barreto, Maria C</style></author><author><style face="normal" font="default" size="100%">Houbraken, Jos</style></author><author><style face="normal" font="default" size="100%">Samson, Robert A</style></author><author><style face="normal" font="default" size="100%">Brito, Dulce</style></author><author><style face="normal" font="default" size="100%">Gadanho, Mário</style></author><author><style face="normal" font="default" size="100%">San Romão, Maria V</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Unveiling the fungal mycobiota present throughout the cork stopper manufacturing process</style></title><secondary-title><style face="normal" font="default" size="100%">FEMS Microbiology Ecology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Biodiversity</style></keyword><keyword><style  face="normal" font="default" size="100%">cloning</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork</style></keyword><keyword><style  face="normal" font="default" size="100%">denaturing gel electrophoresis</style></keyword><keyword><style  face="normal" font="default" size="100%">Denaturing Gradient Gel Electrophoresis</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungal</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungal: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">fungi</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungi: classification</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungi: isolation &amp; purification</style></keyword><keyword><style  face="normal" font="default" size="100%">humidity</style></keyword><keyword><style  face="normal" font="default" size="100%">Industry</style></keyword><keyword><style  face="normal" font="default" size="100%">Metagenome</style></keyword><keyword><style  face="normal" font="default" size="100%">mycobiota diversity</style></keyword><keyword><style  face="normal" font="default" size="100%">Penicillium</style></keyword><keyword><style  face="normal" font="default" size="100%">Penicillium: classification</style></keyword><keyword><style  face="normal" font="default" size="100%">Penicillium: isolation &amp; purification</style></keyword><keyword><style  face="normal" font="default" size="100%">phylogeny</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Bark</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Bark: microbiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Portugal</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: microbiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Ribosomal Spacer</style></keyword><keyword><style  face="normal" font="default" size="100%">Ribosomal Spacer: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Spain</style></keyword><keyword><style  face="normal" font="default" size="100%">Temperature</style></keyword><keyword><style  face="normal" font="default" size="100%">uncultivable fungal species</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year></dates><volume><style face="normal" font="default" size="100%">82</style></volume><pages><style face="normal" font="default" size="100%">202-214</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">A particular fungal population is present in the main stages of the manufacturing process of cork discs. Its diversity was studied using both dependent (isolation) and independent culture methods (denaturing gel gradient electrophoresis and cloning of the ITS1-5.8S-ITS2 region). The mycobiota in the samples taken in the stages before and after the first boiling seems to be distinct from the population in the subsequent manufacturing stages. Most isolated fungi belong to the genera Penicillium, Eurotium and Cladosporium. The presence of uncultivable fungi, Ascomycota and endophytes in raw cork was confirmed by sequencing. The samples taken after the first boiling contained uncultivable fungi, but in a few samples some isolated fungi were also detected. The main taxa present in the following stages were Chrysonilia sitophila, Penicillium glabrum and Penicillium spp. All applied techniques had complementary outcomes. The main factors driving the shift in cork fungal colonization seem to be the high levels of humidity and temperature to which the slabs are subjected during the boiling process.</style></abstract><accession-num><style face="normal" font="default" size="100%">22630140</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Bergero, R</style></author><author><style face="normal" font="default" size="100%">Perotto, S</style></author><author><style face="normal" font="default" size="100%">Girlanda, M</style></author><author><style face="normal" font="default" size="100%">Vidano, G</style></author><author><style face="normal" font="default" size="100%">Luppi, a M</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Ericoid mycorrhizal fungi are common root associates of a Mediterranean ectomycorrhizal plant (Quercus ilex)</style></title><secondary-title><style face="normal" font="default" size="100%">Molecular Ecology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">ectomycorrhizas</style></keyword><keyword><style  face="normal" font="default" size="100%">Erica arborea</style></keyword><keyword><style  face="normal" font="default" size="100%">ericoid fungi</style></keyword><keyword><style  face="normal" font="default" size="100%">fungi</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungi: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungi: isolation &amp; purification</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungi: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic</style></keyword><keyword><style  face="normal" font="default" size="100%">Mediterranean ecosystem</style></keyword><keyword><style  face="normal" font="default" size="100%">Mediterranean Region</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular Sequence Data</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Diseases</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Diseases: microbiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Roots</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Roots: microbiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Polymorphism</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">Restriction Fragment Length</style></keyword><keyword><style  face="normal" font="default" size="100%">Ribosomal Spacer</style></keyword><keyword><style  face="normal" font="default" size="100%">Trees</style></keyword><keyword><style  face="normal" font="default" size="100%">Trees: microbiology</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2000</style></year></dates><publisher><style face="normal" font="default" size="100%">Blackwell Science Ltd</style></publisher><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">1639-1649</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Mycorrhiza samples of neighbouring Quercus ilex and Erica arborea plants collected in a postcutting habitat were processed to see whether plants differing in mycorrhizal status harbour the same root endophytes. Three experiments were performed in parallel: (i) isolation, identification and molecular characterization of fungi from surface-sterilized roots of both plant species; (ii) re-inoculation of fungal isolates on axenic E. arborea and Q. ilex seedlings; (iii) direct inoculation of field-collected Q. ilex ectomycorrhizas onto E. arborea seedlings. About 70 and 150 fungal isolates were obtained from roots of Q. ilex and E. arborea, respectively. Among them, Oidiodendron species and five cultural morphotypes of sterile isolates formed typical ericoid mycorrhizas on E. arborea in vitro. Fungi with such mycorrhizal ability were derived from both host plants. Isolates belonging to one of these morphotypes (sd9) also exhibited an unusual pattern of colonization, with an additional extracellular hyphal net. Ericoid mycorrhizas were also readily obtained by direct inoculation of E. arborea seedlings with Q. ilex ectomycorrhizal tips. Polymerase chain–restriction fragment length polymorphism and random amplified polymorphic DNA analyses of the shared sterile morphotypes demonstrate, in the case of sd9, the occurrence of the same genet on the two host plants. These results indicate that ericoid mycorrhizal fungi associate with ectomycorrhizal roots, and the ecological significance of this finding is discussed.</style></abstract><accession-num><style face="normal" font="default" size="100%">11050558</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Bergero, R.</style></author><author><style face="normal" font="default" size="100%">Perotto, S.</style></author><author><style face="normal" font="default" size="100%">Girlanda, M.</style></author><author><style face="normal" font="default" size="100%">Vidano, G.</style></author><author><style face="normal" font="default" size="100%">Luppi, a M.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Ericoid mycorrhizal fungi are common root associates of a Mediterranean ectomycorrhizal plant (Quercus ilex)</style></title><secondary-title><style face="normal" font="default" size="100%">Molecular Ecology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">ectomycorrhizas</style></keyword><keyword><style  face="normal" font="default" size="100%">Erica arborea</style></keyword><keyword><style  face="normal" font="default" size="100%">ericoid fungi</style></keyword><keyword><style  face="normal" font="default" size="100%">fungi</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungi: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungi: isolation &amp; purification</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungi: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic</style></keyword><keyword><style  face="normal" font="default" size="100%">Mediterranean ecosystem</style></keyword><keyword><style  face="normal" font="default" size="100%">Mediterranean Region</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular Sequence Data</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Diseases</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Diseases: microbiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Roots</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Roots: microbiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Polymorphism</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">Restriction Fragment Length</style></keyword><keyword><style  face="normal" font="default" size="100%">Ribosomal Spacer</style></keyword><keyword><style  face="normal" font="default" size="100%">Trees</style></keyword><keyword><style  face="normal" font="default" size="100%">Trees: microbiology</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2000</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2000///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/11050558http://dx.doi.org/10.1046/j.1365-294x.2000.01059.x</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">1639 - 1649</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Mycorrhiza samples of neighbouring Quercus ilex and Erica arborea plants collected in a postcutting habitat were processed to see whether plants differing in mycorrhizal status harbour the same root endophytes. Three experiments were performed in parallel: (i) isolation, identification and molecular characterization of fungi from surface-sterilized roots of both plant species; (ii) re-inoculation of fungal isolates on axenic E. arborea and Q. ilex seedlings; (iii) direct inoculation of field-collected Q. ilex ectomycorrhizas onto E. arborea seedlings. About 70 and 150 fungal isolates were obtained from roots of Q. ilex and E. arborea, respectively. Among them, Oidiodendron species and five cultural morphotypes of sterile isolates formed typical ericoid mycorrhizas on E. arborea in vitro. Fungi with such mycorrhizal ability were derived from both host plants. Isolates belonging to one of these morphotypes (sd9) also exhibited an unusual pattern of colonization, with an additional extracellular hyphal net. Ericoid mycorrhizas were also readily obtained by direct inoculation of E. arborea seedlings with Q. ilex ectomycorrhizal tips. Polymerase chain–restriction fragment length polymorphism and random amplified polymorphic DNA analyses of the shared sterile morphotypes demonstrate, in the case of sd9, the occurrence of the same genet on the two host plants. These results indicate that ericoid mycorrhizal fungi associate with ectomycorrhizal roots, and the ecological significance of this finding is discussed.</style></abstract><issue><style face="normal" font="default" size="100%">10</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Blackwell Science Ltd&lt;br/&gt;accession-num: 11050558</style></notes></record></records></xml>