<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Calderon Guerrero, Carlos</style></author><author><style face="normal" font="default" size="100%">Guenthardt-Goerg, Madeleine S.</style></author><author><style face="normal" font="default" size="100%">Vollenweider, Pierre</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Foliar Symptoms Triggered by Ozone Stress in Irrigated Holm Oaks from the City of Madrid, Spain</style></title><secondary-title><style face="normal" font="default" size="100%">PLOS ONE</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Holm oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Mediterranean climate</style></keyword><keyword><style  face="normal" font="default" size="100%">natural vegetation</style></keyword><keyword><style  face="normal" font="default" size="100%">Ozone</style></keyword><keyword><style  face="normal" font="default" size="100%">Stomatal uptake</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2013///</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">8</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Background: Despite abatement programs of precursors implemented in many industrialized countries, ozone remains the principal air pollutant throughout the northern hemisphere with background concentrations increasing as a consequence of economic development in former or still emerging countries and present climate change. Some of the highest ozone concentrations are measured in regions with a Mediterranean climate but the effect on the natural vegetation is alleviated by low stomatal uptake and frequent leaf xeromorphy in response to summer drought episodes characteristic of this climate. However, there is a lack of understanding of the respective role of the foliage physiology and leaf xeromorphy on the mechanistic effects of ozone in Mediterranean species. Particularly, evidence about morphological and structural changes in evergreens in response to ozone stress is missing. Results: Our study was started after observing ozone -like injury in foliage of holm oak during the assessment of air pollution mitigation by urban trees throughout the Madrid conurbation. Our objectives were to confirm the diagnosis, investigate the extent of symptoms and analyze the ecological factors contributing to ozone injury, particularly, the site water supply. Symptoms consisted of adaxial and intercostal stippling increasing with leaf age. Underlying stippling, cells in the upper mesophyll showed HR-like reactions typical of ozone stress. The surrounding cells showed further oxidative stress markers. These morphological and micromorphological markers of ozone stress were similar to those recorded in deciduous broadleaved species. However, stippling became obvious already at an AOT40 of 21 ppm.h and was primarily found at irrigated sites. Subsequent analyses showed that irrigated trees had their stomatal conductance increased and leaf life -span reduced whereas the leaf xeromorphy remained unchanged. These findings suggest a central role of water availability versus leaf xeromorphy for ozone symptom expression by cell injury in holm oak.</style></abstract><issue><style face="normal" font="default" size="100%">7</style></issue><notes><style face="normal" font="default" size="100%">APSAPSThe following values have no corresponding Zotero field:&lt;br/&gt;pub-location: 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA&lt;br/&gt;publisher: PUBLIC LIBRARY SCIENCE</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Capitani, Donatella</style></author><author><style face="normal" font="default" size="100%">Brilli, Federico</style></author><author><style face="normal" font="default" size="100%">Mannina, Luisa</style></author><author><style face="normal" font="default" size="100%">Proietti, Noemi</style></author><author><style face="normal" font="default" size="100%">Loreto, Francesco</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In Situ Investigation of Leaf Water Status by Portable Unilateral Nuclear Magnetic Resonance</style></title><secondary-title><style face="normal" font="default" size="100%">PLANT PHYSIOLOGY</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Drought stress</style></keyword><keyword><style  face="normal" font="default" size="100%">Mediterranean vegetation</style></keyword><keyword><style  face="normal" font="default" size="100%">natural vegetation</style></keyword><keyword><style  face="normal" font="default" size="100%">nuclear magnetic resonance</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2009</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2009///</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">149</style></volume><pages><style face="normal" font="default" size="100%">1638 - 1647</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">A portable unilateral nuclear magnetic resonance (NMR) instrument was used to detect in field conditions the water status of leaves of herbaceous crops (Zea mays, Phaseolus vulgaris), mesophyllous trees (Populus nigra), and natural Mediterranean vegetation characterized by water-spending shrubs (Cistus incanus) and water-saving sclerophyllous trees (Quercus ilex). A good relationship was observed between NMR signal, leaf relative water content, and leaf transpiration in herbaceous leaves undergoing fast dehydration or slowly developing a drought stress. A relationship was also observed between NMR signal and water potential of Populus leaves during the development of a water stress and when leaves recovered from the stress. In the natural vegetation, the relationship between NMR signal and water status was found in Cistus, the species characterized by high transpiration rates, when measured during a drought stress period and after a rainfall. In the case of the sclerophyllous Quercus, the NMR signal, the relative water content, and the transpiration rate did not change at different leaf water status, possibly because a large amount of water is compartmentalized in cellular structures and macromolecules. The good association between NMR signal and relative water content was lost in leaves exposed for 24 h to dehydration or to an osmotic stress caused by polyethylene glycol feeding. At this time, the transverse relaxation time became longer than in leaves maintained under optimal water conditions, and two indicators of membrane damage, the ion leakage and the emission of products of membrane lipoxygenation \{[\}(Z)-3-hexenal, (Z)-3-hexenol, and (E)-2-hexenol], increased. These results taken all together give information on the physiological state of a leaf under a developing stress and show the usefulness of the NMR instrumentation for screening vegetation health and fitness in natural and cultivated conditions. It is concluded that the portable unilateral NMR instrument may be usefully employed in field conditions to monitor nondestructively the water status of plants and to assist agricultural practices, such as irrigation scheduling, to minimize stomatal closure and the consequent limitation to plant production.</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;pub-location: 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA&lt;br/&gt;publisher: AMER SOC PLANT BIOLOGISTS</style></notes></record></records></xml>