<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Caldeira, Ana Teresa</style></author><author><style face="normal" font="default" size="100%">Santos Arteiro, J. M. M. M.</style></author><author><style face="normal" font="default" size="100%">Coelho, Ana V.</style></author><author><style face="normal" font="default" size="100%">Roseiro, J. Carlos</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Combined use of LC–ESI-MS and antifungal tests for rapid identification of bioactive lipopeptides produced by Bacillus amyloliquefaciens CCMI 1051</style></title><secondary-title><style face="normal" font="default" size="100%">Process Biochemistry</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">antimicrobial activity</style></keyword><keyword><style  face="normal" font="default" size="100%">bacillus amyloliquefaciens</style></keyword><keyword><style  face="normal" font="default" size="100%">Biosurfactants</style></keyword><keyword><style  face="normal" font="default" size="100%">Cyclic lipopeptides</style></keyword><keyword><style  face="normal" font="default" size="100%">Iturin</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2011///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://linkinghub.elsevier.com/retrieve/pii/S1359511311001929http://www.sciencedirect.com/science/article/pii/S1359511311001929</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">46</style></volume><pages><style face="normal" font="default" size="100%">1738 - 1746</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The strain Bacillus amyloliquefaciens CCMI 1051 used in this study has been isolated in our laboratory from healthy Quercus suber in the south of Portugal and shows high levels of antagonistic properties against filamentous fungi that attack forest products industry due to the production of bioactive peptides. A combined use of LC–ESI-MS and antifungal tests allowed a rapid identification of lipopeptides as active compounds produced. Applying autobiographic methods it was possible to obtain active compounds. LC–ESI-MS, a powerful tool for rapid identification, indicates the presence of lipopeptides and MS2 electrospray ionization showed the partial sequence Tyr–Asn–Pro–Glu in the peptidic portion of some compounds produced. The association of mass spectrometry and chromatography, used in parallel with antifungal tests proved to be an efficient approach for the characterization of active lipopeptides without the need of previous total isolation. This methodology can be employed for screening and optimization the production of antifungal iturinic lipopeptides, showing a great potential for future application.</style></abstract><issue><style face="normal" font="default" size="100%">9</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Elsevier Ltd</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Güllüce, M</style></author><author><style face="normal" font="default" size="100%">Adıgüzel, A</style></author><author><style face="normal" font="default" size="100%">Öğütçü, H</style></author><author><style face="normal" font="default" size="100%">Şengül, M</style></author><author><style face="normal" font="default" size="100%">Karaman, İ</style></author><author><style face="normal" font="default" size="100%">Şahin, F</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Antimicrobial effects of Quercus ilex L. extract</style></title><secondary-title><style face="normal" font="default" size="100%">Phytotherapy Research</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">antimicrobial activity</style></keyword><keyword><style  face="normal" font="default" size="100%">medical plant</style></keyword><keyword><style  face="normal" font="default" size="100%">minimal inhibition concentration</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2004</style></year></dates><publisher><style face="normal" font="default" size="100%">John Wiley &amp; Sons, Ltd.</style></publisher><volume><style face="normal" font="default" size="100%">18</style></volume><pages><style face="normal" font="default" size="100%">208-211</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The antimicrobial activities of the methanol extract of Quercus ilex L. (Pırnal oak) leaves were tested in vitro against a wide range of human and plant-associated microorganisms. A total of 132 microbial organisms belonging to 55 bacteria and ﬁve fungi and yeast species were studied using a disc-diffusion method and microdilution assays. The results were evaluated as inhibition zones around the disc impregnated with Q. ilex extract at a concentration of 300 µL/mL. The results showed that Q. ilex did not have any antifungal activities against Alterneria alternata, Aspergillus ﬂavus, Fusarium oxysporum, Penicillum spp., whereas there were inhibition effects on the growth of all Candida albicans isolates. In total 97 bacterial strains (74%) were found to be resistant to Q. ilex extract. The remaining 35 (27%) strains of seven different bacteria genera including Brucella, Bacillus, Enterobacter, Neisseria, Pseudomonas and Escherichia were susceptible to the extract tested. The minimum inhibitory concentrations (MIC) of the extract ranged from 125 to 500 µL/mL. These results suggest that Q. ilex possesses compounds with antibacterial and anticandidal properties. Copyright © 2004 John Wiley &amp; Sons, Ltd.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Mallea, M</style></author><author><style face="normal" font="default" size="100%">Pesando, D</style></author><author><style face="normal" font="default" size="100%">Bernard, P</style></author><author><style face="normal" font="default" size="100%">Khoulalene, B</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Comparison between antifungal and antibacterial activities of several strains of Epicoccum purpurascens from the Mediterranean area.</style></title><secondary-title><style face="normal" font="default" size="100%">Mycopathologia</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">antimicrobial activity</style></keyword><keyword><style  face="normal" font="default" size="100%">cultural characteristics</style></keyword><keyword><style  face="normal" font="default" size="100%">Epicoccum purpurascens</style></keyword><keyword><style  face="normal" font="default" size="100%">intraspecies com- parison</style></keyword><keyword><style  face="normal" font="default" size="100%">metabolites</style></keyword><keyword><style  face="normal" font="default" size="100%">thin layer chromatography</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1991</style></year></dates><volume><style face="normal" font="default" size="100%">115</style></volume><pages><style face="normal" font="default" size="100%">83-88</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The antimicrobial activities of seven Epicoccum purpurascens strains isolated either from evergreen oak leaves (Quercus ilex) collected over a period of one year, or from the atmosphere were compared in vitro. Two strains sporulated and conspicuously inhibited the growth of Staphylococcus aureus and Trichophyton mentagrophytes. Thin-layer chromatographic studies showed the existence of some compounds, such as flavipin, which were common to all the strains. Epicorazine B was present in the extracts of only the two most active strains.</style></abstract><accession-num><style face="normal" font="default" size="100%">1784310</style></accession-num></record></records></xml>