<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Fernandes, Pedro</style></author><author><style face="normal" font="default" size="100%">Costa, Armando</style></author><author><style face="normal" font="default" size="100%">Rocha, Cristina</style></author><author><style face="normal" font="default" size="100%">Santos, Conceição</style></author><author><style face="normal" font="default" size="100%">Al, E. T.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">GENETIC STABILITY EVALUATION OF QUERCUS SUBER L . SOMATIC EMBRYOGENESIS BY RAPD ANALYSIS</style></title><secondary-title><style face="normal" font="default" size="100%">Pakistan Journal of Botany</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">genetic stability</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber (voyant)</style></keyword><keyword><style  face="normal" font="default" size="100%">RAPD</style></keyword><keyword><style  face="normal" font="default" size="100%">Somatic embryogenesis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2011///</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">43</style></volume><pages><style face="normal" font="default" size="100%">2727 - 2731</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">A reliable protocol for adult Quercus suber L. somatic embryogenesis (SE) was developed recently. To evaluate the potential use of this protocol in cork oak forest breeding programs, it is essential to guarantee somatic embryos/emblings genetic stability. Random Amplification of Polymorphic DNA (RAPD) is currently used to assess somaclonal variation providing information on genetic variability of the micropropagation process. In this work, SE was induced from adult trees by growing leaf explants on MS medium supplemented with 2,4-D and zeatin. Embling conversion took place on MS medium without growth regulators. DNA from donor tree, somatic embryos and emblings was used to assess genetic variability by RAPD fingerprinting. Fourteen primers produced 165 genetic loci with high quality and reproducibility. Despite somatic embryos originated some poor quality PCR-profiles, replicable and excellent fingerprints were obtained for both donor plant and embling. Results presented no differences among regenerated emblings and donor plant. Hence, the SE protocol used did not induce, up to moment, any genetic variability, confirming data previously obtained with other molecular/genetic techniques, supporting that this protocol may be used to provide true-to-type plants from important forestry species.</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Conte, L.</style></author><author><style face="normal" font="default" size="100%">Cotti, C.</style></author><author><style face="normal" font="default" size="100%">Cristofolini, G.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Molecular evidence for hybrid origin of Quercus crenata Lam. (Fagaceae) from Q. cerris L. and Q. suber L.</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">DNA markers</style></keyword><keyword><style  face="normal" font="default" size="100%">Hybridization</style></keyword><keyword><style  face="normal" font="default" size="100%">introgression</style></keyword><keyword><style  face="normal" font="default" size="100%">ISSR</style></keyword><keyword><style  face="normal" font="default" size="100%">RAPD</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2007</style></year></dates><publisher><style face="normal" font="default" size="100%">Taylor &amp; Francis</style></publisher><volume><style face="normal" font="default" size="100%">141</style></volume><pages><style face="normal" font="default" size="100%">181-193</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Abstract Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeats (ISSR) markers were employed to examine samples from Quercus cerris, Q. suber and Q. crenata in order to test the hypothesis of the hybrid origin of Q. crenata from Q. cerris and Q. suber in a part of its distribution area where the two putative parents do not overlap at present. Leaves from 21 Q. crenata and 37 Q. cerris individual trees were collected at natural sites in northern Italy, where Q. suber is currently lacking; 21 Q. suber and six Q. crenata plants from central Italy were also analysed. Results from Unweighted Pair Group Method with Arithmetic mean (UPGMA) analysis and principal component analysis (PCA) implied that exchange of neutral markers has been considerable between the three species, while differences in morphological characters have remained comparatively stable. The Mantel test indicated low correlation between RAPD- and ISSR-based similarity matrices, showing that the two screening techniques reveal unrelated estimates of genetic relationships. Hybrid indices computed for both markers displayed an intermediate position of Q. crenata individuals between the two putative parents shifted toward Q. cerris. Results from the present study corroborate the hypothesis of a hybrid origin for Q. crenata occurring in northern Italy, and suggest asymmetrical backcrossing with Q. cerris acting as the recurrent parent.</style></abstract><notes><style face="normal" font="default" size="100%">From Duplicate 2 (Molecular evidence for hybrid origin of Quercus crenata Lam. (Fagaceae) from Q. cerris L. and Q. suber L. - Conte, L; Cotti, C; Cristofolini, G)</style></notes><research-notes><style face="normal" font="default" size="100%">From Duplicate 2 (Molecular evidence for hybrid origin of Quercus crenata Lam. (Fagaceae) from Q. cerris L. and Q. suber L. - Conte, L; Cotti, C; Cristofolini, G)</style></research-notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Conte, L.</style></author><author><style face="normal" font="default" size="100%">Cotti, C.</style></author><author><style face="normal" font="default" size="100%">Cristofolini, G.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Molecular evidence for hybrid origin of Quercus crenata Lam. (Fagaceae) from Q. cerris L. and Q. suber L.</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">DNA markers</style></keyword><keyword><style  face="normal" font="default" size="100%">Hybridization</style></keyword><keyword><style  face="normal" font="default" size="100%">introgression</style></keyword><keyword><style  face="normal" font="default" size="100%">ISSR</style></keyword><keyword><style  face="normal" font="default" size="100%">RAPD</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2007</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2007///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.tandfonline.com/doi/abs/10.1080/11263500701401463http://dx.doi.org/10.1080/11263500701401463</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">141</style></volume><pages><style face="normal" font="default" size="100%">181 - 193</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Abstract Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeats (ISSR) markers were employed to examine samples from Quercus cerris, Q. suber and Q. crenata in order to test the hypothesis of the hybrid origin of Q. crenata from Q. cerris and Q. suber in a part of its distribution area where the two putative parents do not overlap at present. Leaves from 21 Q. crenata and 37 Q. cerris individual trees were collected at natural sites in northern Italy, where Q. suber is currently lacking; 21 Q. suber and six Q. crenata plants from central Italy were also analysed. Results from Unweighted Pair Group Method with Arithmetic mean (UPGMA) analysis and principal component analysis (PCA) implied that exchange of neutral markers has been considerable between the three species, while differences in morphological characters have remained comparatively stable. The Mantel test indicated low correlation between RAPD- and ISSR-based similarity matrices, showing that the two screening techniques reveal unrelated estimates of genetic relationships. Hybrid indices computed for both markers displayed an intermediate position of Q. crenata individuals between the two putative parents shifted toward Q. cerris. Results from the present study corroborate the hypothesis of a hybrid origin for Q. crenata occurring in northern Italy, and suggest asymmetrical backcrossing with Q. cerris acting as the recurrent parent.</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><notes><style face="normal" font="default" size="100%">From Duplicate 2 (Molecular evidence for hybrid origin of Quercus crenata Lam. (Fagaceae) from Q. cerris L. and Q. suber L. - Conte, L; Cotti, C; Cristofolini, G)From Duplicate 2 (Molecular evidence for hybrid origin of Quercus crenata Lam. (Fagaceae) from Q. cerris L. and Q. suber L. - Conte, L; Cotti, C; Cristofolini, G)The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Taylor &amp; Francis</style></notes></record></records></xml>