<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Valero Galván, José</style></author><author><style face="normal" font="default" size="100%">Valledor, Luis</style></author><author><style face="normal" font="default" size="100%">Navarro Cerrillo, Rafael M</style></author><author><style face="normal" font="default" size="100%">Gil Pelegrín, Eustaquio</style></author><author><style face="normal" font="default" size="100%">Jorrín-Novo, Jesús V</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Studies of variability in Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) through acorn protein profile analysis.</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of proteomics</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Acorn proteomics</style></keyword><keyword><style  face="normal" font="default" size="100%">Chromatography</style></keyword><keyword><style  face="normal" font="default" size="100%">Electrophoresis</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene Expression Profiling</style></keyword><keyword><style  face="normal" font="default" size="100%">Holm oak variability</style></keyword><keyword><style  face="normal" font="default" size="100%">Liquid</style></keyword><keyword><style  face="normal" font="default" size="100%">Mass</style></keyword><keyword><style  face="normal" font="default" size="100%">Matrix-Assisted Laser Desorpti</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Polyacrylamide Gel</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex proteomics</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Seeds</style></keyword><keyword><style  face="normal" font="default" size="100%">Seeds: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Spectrometry</style></keyword><keyword><style  face="normal" font="default" size="100%">Tandem mass spectrometry</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year></dates><publisher><style face="normal" font="default" size="100%">Elsevier B.V.</style></publisher><volume><style face="normal" font="default" size="100%">74</style></volume><pages><style face="normal" font="default" size="100%">1244-1255</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Studies of variability in Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.), the dominant tree species in the typical Mediterranean forest, have been carried out by using electrophoresis-based proteomic analysis of acorns. Ten populations distributed throughout the Andalusia region have been surveyed. Acorns were sampled from individual trees and proteins extracted from seed flour by using the TCA-acetone precipitation protocol. Extracts were subjected to SDS-PAGE and 2-DE for protein separation, gel images captured, spot or bands quantified, and subjected to statistical analysis (ANOVA, SOM and clustering). Variable bands or spots among populations were subjected to MALDI-TOF/TOF and LC-MS/MS for identification. The protein yield of the used protocol varied among populations, and it was in the 2.92-5.92 mg/g dry weight range. A total of 23 bands were resolved by SDS-PAGE in the 3-35 kDa Mr range, with 8 and 12, out of the total, showing respectively qualitative and quantitative statistically significant differences among populations. Data allowed grouping populations, with groups being correlated according to geographical location and climate conditions, to northern and southern, as well as the discrimination of both mesic and xeric groups. Acorn flour extracts from the most distant populations were analyzed by 2-DE, and 56 differential spots were proposed as markers of variability. Identified proteins were classified into two principal categories; storage and stress/defense protein. Besides providing the first reference map of mature acorn seeds, the use of SDS-PAGE and proteomics in characterizing natural biodiversity in forest trees will be discussed.</style></abstract><accession-num><style face="normal" font="default" size="100%">21605712</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Gómez, Aranzazu</style></author><author><style face="normal" font="default" size="100%">López, Juan Antonio</style></author><author><style face="normal" font="default" size="100%">Pintos, Beatriz</style></author><author><style face="normal" font="default" size="100%">Camafeita, Emilio</style></author><author><style face="normal" font="default" size="100%">Bueno, Ma Angeles</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Proteomic analysis from haploid and diploid embryos of Quercus suber L. identifies qualitative and quantitative differential expression patterns.</style></title><secondary-title><style face="normal" font="default" size="100%">Proteomics</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">cluster analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Diploidy</style></keyword><keyword><style  face="normal" font="default" size="100%">Electrophoresis</style></keyword><keyword><style  face="normal" font="default" size="100%">Embryonic Development</style></keyword><keyword><style  face="normal" font="default" size="100%">flow cytometry</style></keyword><keyword><style  face="normal" font="default" size="100%">Gametic embryogenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Gel</style></keyword><keyword><style  face="normal" font="default" size="100%">Haploid and diploid embryos</style></keyword><keyword><style  face="normal" font="default" size="100%">Haploidy</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins: analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins: biosynthesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant proteomics</style></keyword><keyword><style  face="normal" font="default" size="100%">Ploidies</style></keyword><keyword><style  face="normal" font="default" size="100%">Ploidy level</style></keyword><keyword><style  face="normal" font="default" size="100%">Principal component analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Proteomics</style></keyword><keyword><style  face="normal" font="default" size="100%">Proteomics: methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber L.</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Seeds</style></keyword><keyword><style  face="normal" font="default" size="100%">Seeds: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Seeds: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Seeds: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Two-Dimensional</style></keyword><keyword><style  face="normal" font="default" size="100%">Up-Regulation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2009</style></year></dates><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">4355-4367</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Quercus suber L. is a Mediterranean forest species with ecological, social and economic value. Clonal propagation of Q. suber elite trees has been successfully obtained from in vitro-derived somatic and gametic embryos. These clonal lines play a main role in breeding and genetic studies of Q. suber. To aid in unravelling diverse genetic and biological unknowns, a proteomic approach is proposed. The proteomic analysis of Q. suber somatic and gametic in vitro culture-derived embryos, based on DIGE and MALDI-MS, has produced for the first time proteomic data on this species. Seventeen differentially expressed proteins have been identified which display significantly altered levels between gametic and somatic embryos. These proteins are involved in a variety of cellular processes, most of which had been neither previously associated with embryo development nor identified in the genus Quercus. Some of these proteins are involved in stress and pollen development and others play a role in the metabolism of tannins and phenylpropanoids, which represent two of the major pathways for the synthesis of cork chemical components. Furthermore, the augmented expression levels found for specific proteins are probably related to the homozygous state of a doubled-haploid sample. Proteins involved in synthesis of cork components can be detected at such early stages of development, showing the potential of the method to be useful in searching for biomarkers related to cork quality.</style></abstract><accession-num><style face="normal" font="default" size="100%">19662628</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Espín, Juan Carlos</style></author><author><style face="normal" font="default" size="100%">González-Barrio, Rocío</style></author><author><style face="normal" font="default" size="100%">Cerdá, Begoña</style></author><author><style face="normal" font="default" size="100%">López-Bote, Clemente</style></author><author><style face="normal" font="default" size="100%">Rey, Ana I</style></author><author><style face="normal" font="default" size="100%">Tomás-Barberán, Francisco a</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Iberian Pig as a Model To Clarify Obscure Points in the Bioavailability and Metabolism of Ellagitannins in Humans</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Agricultural and Food Chemistry</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Animal</style></keyword><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">bile</style></keyword><keyword><style  face="normal" font="default" size="100%">bioavailability</style></keyword><keyword><style  face="normal" font="default" size="100%">Biological Availability</style></keyword><keyword><style  face="normal" font="default" size="100%">Body Fluids</style></keyword><keyword><style  face="normal" font="default" size="100%">Body Fluids: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Cereals</style></keyword><keyword><style  face="normal" font="default" size="100%">Cereals: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">colon</style></keyword><keyword><style  face="normal" font="default" size="100%">diet</style></keyword><keyword><style  face="normal" font="default" size="100%">ellagic acid</style></keyword><keyword><style  face="normal" font="default" size="100%">Ellagitannin</style></keyword><keyword><style  face="normal" font="default" size="100%">gall bladder</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Hydrolyzable Tannins</style></keyword><keyword><style  face="normal" font="default" size="100%">Hydrolyzable Tannins: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Hydrolyzable Tannins: pharmacokinetics</style></keyword><keyword><style  face="normal" font="default" size="100%">intestine</style></keyword><keyword><style  face="normal" font="default" size="100%">metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Models</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Seeds</style></keyword><keyword><style  face="normal" font="default" size="100%">Seeds: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Swine</style></keyword><keyword><style  face="normal" font="default" size="100%">Swine: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Tissue Distribution</style></keyword><keyword><style  face="normal" font="default" size="100%">urolithin</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2007</style></year></dates><publisher><style face="normal" font="default" size="100%">American Chemical Society</style></publisher><volume><style face="normal" font="default" size="100%">55</style></volume><pages><style face="normal" font="default" size="100%">10476-10485</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Ellagitannin-containing foods (strawberries, walnuts, pomegranate, raspberries, oak-aged wine, etc.) have attracted attention due to their cancer chemopreventive, cardioprotective, and antioxidant effects. Ellagitannins (ETs) are not absorbed as such but are metabolized by the intestinal flora to yield urolithins (hydroxydibenzopyran-6-one derivatives). In this study, Iberian pig is used as a model to clarify human ET metabolism. Pigs were fed either cereal fodder or acorns, a rich source of ETs. Plasma, urine, bile, lumen and intestinal tissues (jejunum and colon), feces, liver, kidney, heart, brain, lung, muscle, and subcutaneous fat tissue were analyzed. The results demonstrate that acorn ETs release ellagic acid (EA) in the jejunum, then the intestinal flora metabolizes EA sequentially to yield tetrahydroxy- (urolithin D), trihydroxy- (urolithin C), dihydroxy- (urolithin A), and monohydroxy- (urolithin B) dibenzopyran-6-one metabolites, which were absorbed preferentially when their lipophilicity increased. Thirty-one ET-derived metabolites were detected, including 25 urolithin and 6 EA derivatives. Twenty-six extensively conjugated metabolites were detected in bile, glucuronides and methyl glucuronides of EA and particularly urolithin A, C, and D derivatives, confirming a very active enterohepatic circulation. Urolithins A and B as well as dimethyl-EA-glucuronide were detected in peripheral plasma. The presence of EA metabolites in bile and in urine and its absence in intestinal tissues suggested its absorption in the stomach. Urolithin A was the only metabolite detected in feces and together with its glucuronide was the most abundant metabolite in urine. No metabolites accumulated in any organ analyzed. The whole metabolism of ETs is shown for the first time, confirming previous studies in humans and explaining the long persistency of urolithin metabolites in the body mediated by an active enterohepatic circulation.</style></abstract><accession-num><style face="normal" font="default" size="100%">17990850</style></accession-num><notes><style face="normal" font="default" size="100%">From Duplicate 2 (Iberian Pig as a Model To Clarify Obscure Points in the Bioavailability and Metabolism of Ellagitannins in Humans - Espín, Juan Carlos; González-Barrio, Rocío; Cerdá, Begoña; López-Bote, Clemente; Rey, Ana I; Tomás-Barberán, Francisco A)</style></notes><research-notes><style face="normal" font="default" size="100%">From Duplicate 2 (Iberian Pig as a Model To Clarify Obscure Points in the Bioavailability and Metabolism of Ellagitannins in Humans - Espín, Juan Carlos; González-Barrio, Rocío; Cerdá, Begoña; López-Bote, Clemente; Rey, Ana I; Tomás-Barberán, Francisco A)</style></research-notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Espín, Juan Carlos</style></author><author><style face="normal" font="default" size="100%">González-Barrio, Rocío</style></author><author><style face="normal" font="default" size="100%">Cerdá, Begoña</style></author><author><style face="normal" font="default" size="100%">López-Bote, Clemente</style></author><author><style face="normal" font="default" size="100%">Rey, Ana I.</style></author><author><style face="normal" font="default" size="100%">Tomás-Barberán, Francisco a</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Iberian Pig as a Model To Clarify Obscure Points in the Bioavailability and Metabolism of Ellagitannins in Humans</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Agricultural and Food Chemistry</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Animal</style></keyword><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">bile</style></keyword><keyword><style  face="normal" font="default" size="100%">bioavailability</style></keyword><keyword><style  face="normal" font="default" size="100%">Biological Availability</style></keyword><keyword><style  face="normal" font="default" size="100%">Body Fluids</style></keyword><keyword><style  face="normal" font="default" size="100%">Body Fluids: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Cereals</style></keyword><keyword><style  face="normal" font="default" size="100%">Cereals: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">colon</style></keyword><keyword><style  face="normal" font="default" size="100%">diet</style></keyword><keyword><style  face="normal" font="default" size="100%">ellagic acid</style></keyword><keyword><style  face="normal" font="default" size="100%">Ellagitannin</style></keyword><keyword><style  face="normal" font="default" size="100%">gall bladder</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Hydrolyzable Tannins</style></keyword><keyword><style  face="normal" font="default" size="100%">Hydrolyzable Tannins: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Hydrolyzable Tannins: pharmacokinetics</style></keyword><keyword><style  face="normal" font="default" size="100%">intestine</style></keyword><keyword><style  face="normal" font="default" size="100%">metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Models</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Seeds</style></keyword><keyword><style  face="normal" font="default" size="100%">Seeds: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Swine</style></keyword><keyword><style  face="normal" font="default" size="100%">Swine: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Tissue Distribution</style></keyword><keyword><style  face="normal" font="default" size="100%">urolithin</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2007</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2007///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/17990850http://dx.doi.org/10.1021/jf0723864</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">55</style></volume><pages><style face="normal" font="default" size="100%">10476 - 10485</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Ellagitannin-containing foods (strawberries, walnuts, pomegranate, raspberries, oak-aged wine, etc.) have attracted attention due to their cancer chemopreventive, cardioprotective, and antioxidant effects. Ellagitannins (ETs) are not absorbed as such but are metabolized by the intestinal flora to yield urolithins (hydroxydibenzopyran-6-one derivatives). In this study, Iberian pig is used as a model to clarify human ET metabolism. Pigs were fed either cereal fodder or acorns, a rich source of ETs. Plasma, urine, bile, lumen and intestinal tissues (jejunum and colon), feces, liver, kidney, heart, brain, lung, muscle, and subcutaneous fat tissue were analyzed. The results demonstrate that acorn ETs release ellagic acid (EA) in the jejunum, then the intestinal flora metabolizes EA sequentially to yield tetrahydroxy- (urolithin D), trihydroxy- (urolithin C), dihydroxy- (urolithin A), and monohydroxy- (urolithin B) dibenzopyran-6-one metabolites, which were absorbed preferentially when their lipophilicity increased. Thirty-one ET-derived metabolites were detected, including 25 urolithin and 6 EA derivatives. Twenty-six extensively conjugated metabolites were detected in bile, glucuronides and methyl glucuronides of EA and particularly urolithin A, C, and D derivatives, confirming a very active enterohepatic circulation. Urolithins A and B as well as dimethyl-EA-glucuronide were detected in peripheral plasma. The presence of EA metabolites in bile and in urine and its absence in intestinal tissues suggested its absorption in the stomach. Urolithin A was the only metabolite detected in feces and together with its glucuronide was the most abundant metabolite in urine. No metabolites accumulated in any organ analyzed. The whole metabolism of ETs is shown for the first time, confirming previous studies in humans and explaining the long persistency of urolithin metabolites in the body mediated by an active enterohepatic circulation.</style></abstract><issue><style face="normal" font="default" size="100%">25</style></issue><notes><style face="normal" font="default" size="100%">From Duplicate 2 (Iberian Pig as a Model To Clarify Obscure Points in the Bioavailability and Metabolism of Ellagitannins in Humans - Espín, Juan Carlos; González-Barrio, Rocío; Cerdá, Begoña; López-Bote, Clemente; Rey, Ana I; Tomás-Barberán, Francisco A)From Duplicate 2 (Iberian Pig as a Model To Clarify Obscure Points in the Bioavailability and Metabolism of Ellagitannins in Humans - Espín, Juan Carlos; González-Barrio, Rocío; Cerdá, Begoña; López-Bote, Clemente; Rey, Ana I; Tomás-Barberán, Francisco A)The following values have no corresponding Zotero field:&lt;br/&gt;publisher: American Chemical Society&lt;br/&gt;accession-num: 17990850</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Gonzalez-Benito, M Elena</style></author><author><style face="normal" font="default" size="100%">Prieto, Roberto-Moreno</style></author><author><style face="normal" font="default" size="100%">Herradon, Esther</style></author><author><style face="normal" font="default" size="100%">Martin, Carmen</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cryopreservation of Quercus suber and Quercus ilex embryonic axes: in vitro culture, desiccation and cooling factors.</style></title><secondary-title><style face="normal" font="default" size="100%">Cryo letters</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cryopreservation</style></keyword><keyword><style  face="normal" font="default" size="100%">Cryopreservation: methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Cryoprotective Agents</style></keyword><keyword><style  face="normal" font="default" size="100%">Cryoprotective Agents: pharmacology</style></keyword><keyword><style  face="normal" font="default" size="100%">Culture Techniques</style></keyword><keyword><style  face="normal" font="default" size="100%">Culture Techniques: methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Desiccation</style></keyword><keyword><style  face="normal" font="default" size="100%">Desiccation: methods</style></keyword><keyword><style  face="normal" font="default" size="100%">embryonic axes</style></keyword><keyword><style  face="normal" font="default" size="100%">germination</style></keyword><keyword><style  face="normal" font="default" size="100%">germination temperature</style></keyword><keyword><style  face="normal" font="default" size="100%">Germination: drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">liquid nitrogen</style></keyword><keyword><style  face="normal" font="default" size="100%">plantlet development</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: embryology</style></keyword><keyword><style  face="normal" font="default" size="100%">Seeds</style></keyword><keyword><style  face="normal" font="default" size="100%">Seeds: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Seeds: embryology</style></keyword><keyword><style  face="normal" font="default" size="100%">Survival Rate</style></keyword><keyword><style  face="normal" font="default" size="100%">Temperature</style></keyword><keyword><style  face="normal" font="default" size="100%">water</style></keyword><keyword><style  face="normal" font="default" size="100%">Water: analysis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2002</style></year></dates><volume><style face="normal" font="default" size="100%">23</style></volume><pages><style face="normal" font="default" size="100%">283-290</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">This study examines different factors included in the cryopreservation protocols for Quercus ilex and Q. suber embryonic axes. In vitro incubation temperature played an important role in the appropriate development of Q. ilex axes, as 15 degrees C was superior to 25 degrees C. Q. suber axes proved to be more sensitive to desiccation and cooling. Poor survival (35%) was observed when axes were included into cryovials and then in liquid nitrogen, and none when immersed in sub-cooled liquid nitrogen (-210 degrees C). Q. ilex axes showed poorly organised development in vitro (c. 50% of non-cooled axes showed shoot development). However, c. 80% survival was observed after cryopreservation (either in liquid nitrogen or sub-cooled liquid nitrogen at 0.34 g water / g dry weight), of which c. 15% showed shoot development.</style></abstract><accession-num><style face="normal" font="default" size="100%">12447487</style></accession-num></record></records></xml>