<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Grote, R</style></author><author><style face="normal" font="default" size="100%">Mayrhofer, S</style></author><author><style face="normal" font="default" size="100%">Fischbach, R J</style></author><author><style face="normal" font="default" size="100%">Steinbrecher, R</style></author><author><style face="normal" font="default" size="100%">Staudt, M</style></author><author><style face="normal" font="default" size="100%">Schnitzler, J.-P.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Process-based modelling of isoprenoid emissions from evergreen leaves of Quercus ilex (L.)</style></title><secondary-title><style face="normal" font="default" size="100%">Atmospheric Environment</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">model development</style></keyword><keyword><style  face="normal" font="default" size="100%">monoterpene emission rates</style></keyword><keyword><style  face="normal" font="default" size="100%">monoterpene synthases</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">Seasonality</style></keyword><keyword><style  face="normal" font="default" size="100%">sim-bim2</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2006</style></year></dates><volume><style face="normal" font="default" size="100%">40</style></volume><pages><style face="normal" font="default" size="100%">152-165</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Monoterpenes play an important role in regulating the trace gas composition of the lower troposphere. Therefore, realistic estimates of the daily as well as seasonal variations of monoterpene emission source strength on the Earth surface are required. Monoterpenes are emitted by Holm oak (Quercus ilex L.) and other species lacking speciﬁc foliar terpene storage structures and their development is dependent on light and temperature. In the present work we describe a process-based emission model taking into account the physiological/phenological state of Holm oak leaves and biochemical processes leading to the formation of monoterpenes. The model ‘seasonal isoprenoid synthase model–biochemical isoprenoid biosynthesis model’ (SIM–BIM2) is developed based on a previous version which was used to simulate isoprene emissions from deciduous oaks. The current model considers additional enzymatic reactions in Holm oak chloroplasts that lead to the formation of monoterpenes. The comparison of simulated and measured biochemical properties as well as emission rates displayed that the ability of the model to dynamically adjust monoterpene biosynthesis capacity by modulating the amount of monoterpene synthase activities in dependence of the weather pattern led to realistic simulations of light-dependent monoterpene emission rates. Differences to simulation results obtained by a widely used alternative model [Guenther, A.B., Zimmerman, P.R., Harley, P.C., Monson, R.K., Fall, R., 1993. Isoprene and monoterpene emission rate variability—model evaluations and sensitivity analyses. Journal of Geophysical Research 98, 12609–12617] are discussed.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Fischbach, Robert J.</style></author><author><style face="normal" font="default" size="100%">Zimmer, Wolfgang</style></author><author><style face="normal" font="default" size="100%">Schnitzler, Jörg-Peter</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Isolation and functional analysis of a cDNA encoding a myrcene synthase from holm oak (Quercus ilex L.)</style></title><secondary-title><style face="normal" font="default" size="100%">European Journal of Biochemistry</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">functional expression</style></keyword><keyword><style  face="normal" font="default" size="100%">geranyl diphosphate</style></keyword><keyword><style  face="normal" font="default" size="100%">monoterpene synthases</style></keyword><keyword><style  face="normal" font="default" size="100%">myrcene synthase</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2001</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2001///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1046/j.1432-1033.2001.02519.x</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">268</style></volume><pages><style face="normal" font="default" size="100%">5633 - 5638</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">An 859-bp cDNA segment of a terpene synthase gene was amplified by PCR from the evergreen sclerophyllous holm oak (Quercus ilex L.) using heterologous primers for conserved regions of terpene synthase genes (TPS) in dicotyledonous plants. Based on the sequence of this segment, homologous primers were designed for amplification by RACE-PCR of a cDNA segment carrying the monoterpene synthase gene myrS. The gene encodes a protein of 597 amino acids including an N-terminal putative plastid transit peptide. The gene without the segment encoding the transit peptide was cloned by PCR into a bacterial expression vector. Expression in Escherichia coli yielded an active monoterpene synthase, which converted geranyl diphosphate (GDP) predominantly into the acyclic monoterpene myrcene and to a very small extent into cyclic monoterpenes. Sequence comparison with previously cloned monoterpene synthases revealed that the myrcene synthase from Q. ilex belongs to the TPSb subfamily.</style></abstract><issue><style face="normal" font="default" size="100%">21</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Blackwell Science Ltd</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Fischbach, Robert J</style></author><author><style face="normal" font="default" size="100%">Zimmer, Wolfgang</style></author><author><style face="normal" font="default" size="100%">Schnitzler, Jörg-Peter</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Isolation and functional analysis of a cDNA encoding a myrcene synthase from holm oak (Quercus ilex L.)</style></title><secondary-title><style face="normal" font="default" size="100%">European Journal of Biochemistry</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">functional expression</style></keyword><keyword><style  face="normal" font="default" size="100%">geranyl diphosphate</style></keyword><keyword><style  face="normal" font="default" size="100%">monoterpene synthases</style></keyword><keyword><style  face="normal" font="default" size="100%">myrcene synthase</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2001</style></year></dates><publisher><style face="normal" font="default" size="100%">Blackwell Science Ltd</style></publisher><volume><style face="normal" font="default" size="100%">268</style></volume><pages><style face="normal" font="default" size="100%">5633-5638</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">An 859-bp cDNA segment of a terpene synthase gene was amplified by PCR from the evergreen sclerophyllous holm oak (Quercus ilex L.) using heterologous primers for conserved regions of terpene synthase genes (TPS) in dicotyledonous plants. Based on the sequence of this segment, homologous primers were designed for amplification by RACE-PCR of a cDNA segment carrying the monoterpene synthase gene myrS. The gene encodes a protein of 597 amino acids including an N-terminal putative plastid transit peptide. The gene without the segment encoding the transit peptide was cloned by PCR into a bacterial expression vector. Expression in Escherichia coli yielded an active monoterpene synthase, which converted geranyl diphosphate (GDP) predominantly into the acyclic monoterpene myrcene and to a very small extent into cyclic monoterpenes. Sequence comparison with previously cloned monoterpene synthases revealed that the myrcene synthase from Q. ilex belongs to the TPSb subfamily.</style></abstract></record></records></xml>