<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Pérez, Marta</style></author><author><style face="normal" font="default" size="100%">Viejo, Marcos</style></author><author><style face="normal" font="default" size="100%">LaCuesta, Maite</style></author><author><style face="normal" font="default" size="100%">Toorop, Peter</style></author><author><style face="normal" font="default" size="100%">Cañal, Maria Jesus</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Epigenetic and hormonal profile during maturation of Quercus Suber L. somatic embryos.</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of plant physiology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">ABA</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA methylation</style></keyword><keyword><style  face="normal" font="default" size="100%">Immunolocalization</style></keyword><keyword><style  face="normal" font="default" size="100%">maturation</style></keyword><keyword><style  face="normal" font="default" size="100%">Somatic embryogenesis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year></dates><volume><style face="normal" font="default" size="100%">173</style></volume><pages><style face="normal" font="default" size="100%">51-61</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Somatic embryogenesis is a powerful alternative to conventional mass propagation of Quercus suber L. However, poor quality and incomplete maturation of somatic embryos restrict any application. Given that epigenetic and hormonal control govern many developmental stages, including maturation of zygotic embryos, global DNA methylation and abscisic acid (ABA) were analyzed during development and maturation of cork oak somatic embryos. Our results indicated that development of somatic embryos concurred with a decrease in 5-mdC. In contrast, endogenous ABA content showed a transient increase with a peak in immature E2 embryos denoting the onset of the maturation phase. A cold stratification phase was necessary for embryos to acquire germination ability, which coincided with a significant decrease in 5-mdC and ABA content. Immunohistochemical analyses showed that there was a specific spatial-temporal regulation during embryogenesis, particularly after the cold treatment. The acquisition of germination capacity concurred with a general low 5-mdC signal in the root meristem, while retention of the 5-mdC signal was mainly located in the shoot meristem and provascular tissues. Conversely, ABA immunolocalization was mainly located in the root and shoot apical meristems. Furthermore, a strong decrease in the ABA signal was observed in the root cap after the stratification treatment suggesting a role for the root cap during development of somatic embryos. These results suggest that, in addition to ABA, epigenetic control appears to play an important role for the correct maturation and subsequent germination of cork oak somatic embryos.</style></abstract><accession-num><style face="normal" font="default" size="100%">25462078</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>47</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Garcia-Martin, G.</style></author><author><style face="normal" font="default" size="100%">Fernandez-Galiano, E.</style></author><author><style face="normal" font="default" size="100%">Mauri, P. V.</style></author><author><style face="normal" font="default" size="100%">Somática, Embriogénesis</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Multiplicación vegetativa de alcornoque mediante embriogénesis somática.</style></title><secondary-title><style face="normal" font="default" size="100%">II Congreso Forestal Español</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">ABA</style></keyword><keyword><style  face="normal" font="default" size="100%">BaP</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">germination</style></keyword><keyword><style  face="normal" font="default" size="100%">liquid culture</style></keyword><keyword><style  face="normal" font="default" size="100%">maturation</style></keyword><keyword><style  face="normal" font="default" size="100%">somatic embryos</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1997</style></year><pub-dates><date><style  face="normal" font="default" size="100%">1997///</style></date></pub-dates></dates><pages><style face="normal" font="default" size="100%">249 - 252</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">In order to obtain cork oak clones from selected individuals, somatic embryogenesis was induced in leaves. Somatic embryos can be continuously subcultured with high proliferation rates. This culture system has sorne limiting factors: low percentage of mature embryos obtained, low rate of shoot emergence during germination and high labour and time consumed. Exogenous addition of ABA (l0-6M) concentration during proliferation increased maturation rateo In the same way addition of. BAP (0,001 mg/l) had a positive effect on shoot production. With the aim to obtain a first approach to automation the best culture media for both culture cycles of maturation in liquid medium were determined.</style></abstract><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;periodical: II Congreso Forestal Español&lt;br/&gt;pub-location: Pamplona</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>3</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">GARCÍA-MARTÍN, G</style></author><author><style face="normal" font="default" size="100%">Fernandez-Galiano, E</style></author><author><style face="normal" font="default" size="100%">Mauri, P V</style></author><author><style face="normal" font="default" size="100%">Somática, Embriogénesis</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Multiplicación vegetativa de alcornoque mediante embriogénesis somática.</style></title><secondary-title><style face="normal" font="default" size="100%">II Congreso Forestal Español</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">ABA</style></keyword><keyword><style  face="normal" font="default" size="100%">BaP</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">germination</style></keyword><keyword><style  face="normal" font="default" size="100%">liquid culture</style></keyword><keyword><style  face="normal" font="default" size="100%">maturation</style></keyword><keyword><style  face="normal" font="default" size="100%">somatic embryos</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1997</style></year></dates><pub-location><style face="normal" font="default" size="100%">Pamplona</style></pub-location><pages><style face="normal" font="default" size="100%">249-252</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">In order to obtain cork oak clones from selected individuals, somatic embryogenesis was induced in leaves. Somatic embryos can be continuously subcultured with high proliferation rates. This culture system has sorne limiting factors: low percentage of mature embryos obtained, low rate of shoot emergence during germination and high labour and time consumed. Exogenous addition of ABA (l0-6M) concentration during proliferation increased maturation rateo In the same way addition of. BAP (0,001 mg/l) had a positive effect on shoot production. With the aim to obtain a first approach to automation the best culture media for both culture cycles of maturation in liquid medium were determined.</style></abstract></record></records></xml>