<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Garcia-Valverde, R.</style></author><author><style face="normal" font="default" size="100%">Nieto, R.</style></author><author><style face="normal" font="default" size="100%">Aguilera, J. F.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Effects of herbage ingestion upon ileal digestibility of amino acids in heavy Iberian pigs fed on an acorn-based diet</style></title><secondary-title><style face="normal" font="default" size="100%">JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">acorns</style></keyword><keyword><style  face="normal" font="default" size="100%">amino acids</style></keyword><keyword><style  face="normal" font="default" size="100%">digestibility</style></keyword><keyword><style  face="normal" font="default" size="100%">herbage</style></keyword><keyword><style  face="normal" font="default" size="100%">iberian pig</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2010</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2010///</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">94</style></volume><pages><style face="normal" font="default" size="100%">e203 - e214</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">P&gt;We conducted two experiments with heavy Iberian pigs to determine the ileal digestibility of amino acids (AA) in acorns and freshly cut herbage, and the effects of adding fresh herbage upon the supply of ileal digestible AA when pigs were fed on holm-oak acorns. In Experiment 1, carried out in cannulated pigs of 107 kg bodyweight (BW), daily intake of acorns reached 44.9 g DM/kg0.75 BW. Arg, His and Thr showed the lowest apparent ileal digestibility (AID) values, whereas Met, the branched-chain AA and Phe had the highest coefficients. The AID of total EAA was 0.716 but only 0.222 for NEAA. Most of the digestive and absorptive processes of acorn protein occurred before the hindgut. Acorn provides (per kg DM) 2.27 g apparent ileal digestible Lys and 22.7 g apparent total digestible AA. Standardized ileal digestibility (SID) values for EAA, NEAA and total AA were 0.924 +/- 0.020, 0.784 +/- 0.041 and 0.860 +/- 0.029. In Experiment 2 fresh herbage was given to six cannulated Iberian pigs of 140 kg either as a single feed (13.7 g DM/kg0.75 BW) or as a supplement to acorns (28.4 g DM/kg0.75 BW). When only freshly cut forage was offered the AID of the EAA, NEAA and total AA was close to 0.65 and supplied (per kg DM ingested) 5.61 g AID Lys and 91.7 g digestible AA. Standardized ileal values were 0.744 +/- 0.023, 0.912 +/- 0.038 and 0.831 +/- 0.030 respectively. The addition of fresh forage to the acorns led to a significant decrease in AID of AA in acorn due to digesta transfer to the hindgut: His (p &lt; 0.01), Met (p &lt; 0.001), Phe (p = 0.092), Thr (p &lt; 0.05) and Val (p &lt; 0.05), but Arg, Lys and the branched-chain AA remained unaffected. The main contribution of herbage to AA nutrition of the grazing Iberian pig relies mainly on increasing the supply of digestible AA for pig tissues.</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;pub-location: COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA&lt;br/&gt;publisher: WILEY-BLACKWELL PUBLISHING, INC</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Pintos, Beatriz</style></author><author><style face="normal" font="default" size="100%">Manzanera, Jose a</style></author><author><style face="normal" font="default" size="100%">Bueno, M. Angeles</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Oak somatic and gametic embryos maturation is affected by charcoal and specific aminoacids mixture</style></title><secondary-title><style face="normal" font="default" size="100%">Annals of Forest Science</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">activated charcoal</style></keyword><keyword><style  face="normal" font="default" size="100%">amino acids</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">gametic embryo</style></keyword><keyword><style  face="normal" font="default" size="100%">plantlet acclimation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2010</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2010///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.springerlink.com/openurl.asp?id=doi:10.1051/forest/2009098</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">67</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">• Development of both somatic and gametic embryogenesis has many applications in clonal forestry and genetic improvement, for instance as mass-propagation of genetically improved plants and production of pure lines through doubled-haploid plant regeneration from gametic embryos. • The goal of this work was to improve growth, maturation and plantlet regeneration of cork oak (Quercus suber L.) embryos from both somatic and gametic origin. • Activated charcoal promoted a signiﬁcant increase in growth in terms of relative size and weight of both somatic and doubled-haploid embryos, as well as a more eﬃcient control of secondary somatic embryogenesis during development. A signiﬁcant interaction was also observed with amino acid nutrition. While some amino acids (i.e., glutamine, arginine or asparagine) did not show signiﬁcant diﬀerences with the controls, a mixture of these three amino acids or gamma amino butyric acid stimulated embryo growth. The highest survival rate during acclimation of plantlets from both somatic and doubled-haploid origin was obtained when the embryos had been previously cultured on basal medium with 3% sucrose and 1% activated charcoal for two months at 4 ◦ C and germinated on medium supplemented with 6-benzylaminopurine and indole-3-butyric acid. • We obtained more than 900 cork oak plantlets acclimated from several embryogenic lines, with a high survival rate, demonstrating that this methodology is applicable for large scale plantlet production. We also report the ﬁrst regeneration of doubled-haploid plantlets in cork oak.</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Aguilera, J. F.</style></author><author><style face="normal" font="default" size="100%">Nieto, R.</style></author><author><style face="normal" font="default" size="100%">Rivera, M.</style></author><author><style face="normal" font="default" size="100%">Garcıa, M. A.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Amino acid availability and energy value of acorn in the Iberian pig</style></title><secondary-title><style face="normal" font="default" size="100%">Livestock Production Science</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">acorn</style></keyword><keyword><style  face="normal" font="default" size="100%">amino acids</style></keyword><keyword><style  face="normal" font="default" size="100%">energy value</style></keyword><keyword><style  face="normal" font="default" size="100%">pig-feeding and nutrition</style></keyword><keyword><style  face="normal" font="default" size="100%">protein quality</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2002</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2002///</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">77</style></volume><pages><style face="normal" font="default" size="100%">227 - 239</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Two experiments have been performed to determine amino acid digestibility and energy value of acorn (Quercus rotundifolia L.) for Iberian pigs. In experiment 1 ileal digestibility of amino acids was determined in ﬁve ileum-cannulated growing pigs fed whole ground acorn. An estimation of endogenous protein losses was carried out by feeding a protein-free diet, added (180 g/kg) or not with acorn hulls. True ileal digestibility of all essential amino acids measured was high (0.798–1.03) except for arginine (0.523), lysine (0.601), isoleucine (0.760), leucine (0.777) and phenylalanine (0.716). The average value for the digestibility of the sum of amino acids was 0.664. Lysine was the ﬁrst limiting amino acid compared with the amino acid proﬁle of the ideal protein for pigs. In experiment 2, total tract digestibility of nutrients and N balance were determined in ﬁve, non-cannulated pigs offered whole acorns. High coefﬁcients for the apparent total tract digestibility of dry matter (ApDM) (0.830) and energy metabolizability (ME/GE; 0.805) were found. ME content for acorn kernel was 16.6 MJ/kg DM, while the apparent total tract digestibility of total N (ApTN) was as low as 0.408. However, a positive N retention was observed (3.7 g/day). Comparing experiments 1 vs. 2, inclusion of acorn hulls resulted in a marked decrease in DM intake, ApTN and in N retention (25.1 g/day, in total tract data from experiment 1). The negative effects of acorn hulls on protein digestibility and N balance were corroborated in rats given similar amounts of either whole ground acorn or ground acorn kernel. Dietary supplementation with lysine is proposed to achieve a higher efﬁciency of protein accretion in the Iberian pig when fed on acorn.</style></abstract></record></records></xml>