<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sebastiana, Mónica</style></author><author><style face="normal" font="default" size="100%">Figueiredo, Andreia</style></author><author><style face="normal" font="default" size="100%">Monteiro, Filipa</style></author><author><style face="normal" font="default" size="100%">Martins, Joana</style></author><author><style face="normal" font="default" size="100%">Franco, Catarina</style></author><author><style face="normal" font="default" size="100%">Coelho, Ana Varela</style></author><author><style face="normal" font="default" size="100%">Vaz, Fátima</style></author><author><style face="normal" font="default" size="100%">Simões, Tânia</style></author><author><style face="normal" font="default" size="100%">Penque, Deborah</style></author><author><style face="normal" font="default" size="100%">Pais, Maria Salomé</style></author><author><style face="normal" font="default" size="100%">Ferreira, Sílvia</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A possible approach for gel-based proteomic studies in recalcitrant woody plants.</style></title><secondary-title><style face="normal" font="default" size="100%">SpringerPlus</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">2-de</style></keyword><keyword><style  face="normal" font="default" size="100%">ectomycorrhizal roots</style></keyword><keyword><style  face="normal" font="default" size="100%">grapevine</style></keyword><keyword><style  face="normal" font="default" size="100%">Mass spectrometry</style></keyword><keyword><style  face="normal" font="default" size="100%">oak</style></keyword><keyword><style  face="normal" font="default" size="100%">pine</style></keyword><keyword><style  face="normal" font="default" size="100%">protein extraction</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year></dates><volume><style face="normal" font="default" size="100%">2</style></volume><pages><style face="normal" font="default" size="100%">210</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Woody plants are particularly difficult to investigate due to high phenolic, resin, and tannin contents and laborious sample preparation. In particular, protein isolation from woody plants for two-dimensional gel electrophoresis (2-DE) is challenging as secondary metabolites negatively interfere with protein extraction and separation. In this study, three protein extraction protocols, using TCA, phenol and ethanol as precipitation or extraction agents, were tested in order to select the more efficient for woody recalcitrant plant gel-based proteomics. Grapevine leaves, pine needles and cork oak ectomycorrhizal roots were used to represent woody plant species and tissues. The phenol protocol produced higher quality 2-DE gels, with increased number of resolved spots, better spot focusing and representation of all molecular mass and isoelectric point ranges tested. In order to test the compatibility of the phenol extracted proteomes with protein identification several spots were excised from the phenol gels and analyzed by mass spectrometry (MALDI-TOF/TOF). Regardless the incomplete genome/protein databases for the plant species under analysis, 49 proteins were identified by Peptide Mass Fingerprint (PMF). Proteomic data have been deposited to the ProteomeXchange with identifier PXD000224. Our results demonstrate the complexity of protein extraction from woody plant tissues and the suitability of the phenol protocol for obtaining high quality protein extracts for efficient 2-DE separation and downstream applications such as protein identification by mass spectrometry.</style></abstract><accession-num><style face="normal" font="default" size="100%">23724367</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Kesselmeier, J</style></author><author><style face="normal" font="default" size="100%">Bode, K</style></author><author><style face="normal" font="default" size="100%">Hofmann, U</style></author><author><style face="normal" font="default" size="100%">Mtjller, H</style></author><author><style face="normal" font="default" size="100%">Schafer, L</style></author><author><style face="normal" font="default" size="100%">Wolf, A</style></author><author><style face="normal" font="default" size="100%">Ciccioli, P</style></author><author><style face="normal" font="default" size="100%">Cecinato, A</style></author><author><style face="normal" font="default" size="100%">Frattoni, M</style></author><author><style face="normal" font="default" size="100%">Foster, P</style></author><author><style face="normal" font="default" size="100%">Dutaur, L</style></author><author><style face="normal" font="default" size="100%">Torreq, L</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">EMISSION OF SHORT CHAINED ORGANIC ACIDS , ALDEHYDES AND MONOTERPENES FROM QUERCUS ILEX L . AND PINUS PINEA L . IN RELATION TO PHYSIOLOGICAL ACTIVITIES , CARBON BUDGET AND EMISSION ALGORITHMS</style></title><secondary-title><style face="normal" font="default" size="100%">Atmospheric Environment</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">aldehydes</style></keyword><keyword><style  face="normal" font="default" size="100%">carbon budget</style></keyword><keyword><style  face="normal" font="default" size="100%">coniferous</style></keyword><keyword><style  face="normal" font="default" size="100%">deciduous</style></keyword><keyword><style  face="normal" font="default" size="100%">light</style></keyword><keyword><style  face="normal" font="default" size="100%">Monoterpenes</style></keyword><keyword><style  face="normal" font="default" size="100%">oak</style></keyword><keyword><style  face="normal" font="default" size="100%">organic acids</style></keyword><keyword><style  face="normal" font="default" size="100%">pine</style></keyword><keyword><style  face="normal" font="default" size="100%">Pinus pinea L</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex L</style></keyword><keyword><style  face="normal" font="default" size="100%">Temperature</style></keyword><keyword><style  face="normal" font="default" size="100%">Volatile Organic Compounds</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1997</style></year></dates><volume><style face="normal" font="default" size="100%">31</style></volume><pages><style face="normal" font="default" size="100%">119-133</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">We report on the emission of monoterpenes, short-chained organic acids and aldehydes from Mediterranean oak (Quercus ilex L.) and pine (Pinus pinea L.). All studies were done with dynamic cuvettes enclosing intact branches at the top of the canopy flushed with ambient air. Daily trends are compared with the photosynthetic active radiation (PAR), leaf temperature and the physiological activities of the enclosed branches, i.e. assimilation and transpiration, with special attention on the carbon budget. Oak emits monoterpenes in high amounts, up to 2% of the assimilated carbon. As compared with monoterpenes, short-chained organic acids and aldehydes are of minor importance for oak. However, on a leaf dry-weight basis equal amounts of acids and aldehydes are released from oak and pine. As pine emitted only low amounts of terpenes (below 0.2% of the assimilated carbon) the release of terpenes and oxygenated compounds is of equal importance for this species. A comparison of a modelled light and temperature driven emission with the observed volatile organic compounds (VOC) emissions showed good agreement for monoterpenes as well as for organic acids emitted in the case of oak. For pine only the release of acids showed an adequate relation to the algorithm data, whereas the terpene emissions seemed to be dominated by temperature effects</style></abstract></record></records></xml>