<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Rico, L</style></author><author><style face="normal" font="default" size="100%">Ogaya, R</style></author><author><style face="normal" font="default" size="100%">Terradas, J</style></author><author><style face="normal" font="default" size="100%">Penuelas, J</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Community structures of N-2-fixing bacteria associated with the phyllosphere of a Holm oak forest and their response to drought</style></title><secondary-title><style face="normal" font="default" size="100%">PLANT BIOLOGY</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Bacterial TRF richness</style></keyword><keyword><style  face="normal" font="default" size="100%">diazotrophic community</style></keyword><keyword><style  face="normal" font="default" size="100%">Drought</style></keyword><keyword><style  face="normal" font="default" size="100%">foliar phyllosphere</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">t-RFLP</style></keyword><keyword><style  face="normal" font="default" size="100%">Trees</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year></dates><publisher><style face="normal" font="default" size="100%">WILEY-BLACKWELL</style></publisher><pub-location><style face="normal" font="default" size="100%">111 RIVER ST, HOBOKEN 07030-5774, NJ USA</style></pub-location><volume><style face="normal" font="default" size="100%">16</style></volume><pages><style face="normal" font="default" size="100%">586-593</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Biological nitrogen (N) fixation is a key pathway in terrestrial ecosystems and is therefore critical for understanding the responses of ecosystems to global environmental changes. The free-living diazotrophic community is distributed along the canopy-to-soil profile, but the ecological significance of epiphyllic N-2 fixers, despite their functional relevance, on plant foliar surfaces remains very poorly understood compared with the N-2-fixing community in forest litter and soils. We assessed the community structure of N-2 fixers and overall bacteria by genetic fingerprinting (t-RFLP) to explore the seasonal successional patterns of the microbial community in the natural phyllosphere of a Holm oak (Quercus ilex) forest submitted to 12-year field experiment of rain exclusion mimicking the conditions of drought projected for the coming decades. Leaves of Holm oak were analysed in different seasons over a period of 1.5years. The bacterial community of the phyllosphere did not correspond to the surrounding soil biome in the same area. These analyses provided field evidence for the presence of free-living diazotrophs associated with the tissues of leaves of Holm oak, the dominant tree species of many Mediterranean forests. The results also revealed that the community composition is affected seasonally and inter-annually by the environment, and that the composition shifts in response to climate change. Drought treatment increased the richness of the epiphyllic microbial community, especially during the summer. These changes were associated with higher C:N ratios of leaves observed in response to drought in semiarid areas. This epiphyllic microbiota that can potentially fix N-2 extends the capacity of plants to adapt to the environment.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Penuelas, J</style></author><author><style face="normal" font="default" size="100%">Rico, L</style></author><author><style face="normal" font="default" size="100%">Ogaya, R</style></author><author><style face="normal" font="default" size="100%">Jump, A S</style></author><author><style face="normal" font="default" size="100%">Terradas, J</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Summer season and long-term drought increase the richness of bacteria and fungi in the foliar phyllosphere of Quercus ilex in a mixed Mediterranean forest</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Biology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Bacteria</style></keyword><keyword><style  face="normal" font="default" size="100%">Bacteria: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Bacteria: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Bacterial</style></keyword><keyword><style  face="normal" font="default" size="100%">Bacterial TRF richness</style></keyword><keyword><style  face="normal" font="default" size="100%">Bacterial: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Biodiversity</style></keyword><keyword><style  face="normal" font="default" size="100%">colonisation time</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Drought</style></keyword><keyword><style  face="normal" font="default" size="100%">Droughts</style></keyword><keyword><style  face="normal" font="default" size="100%">endophytes</style></keyword><keyword><style  face="normal" font="default" size="100%">epiphytes</style></keyword><keyword><style  face="normal" font="default" size="100%">foliar and epiphytic microbial diversity</style></keyword><keyword><style  face="normal" font="default" size="100%">foliar phyllosphere</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungal</style></keyword><keyword><style  face="normal" font="default" size="100%">fungal TRF richness</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungal: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">fungi</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungi: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Fungi: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Mediterranean Region</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: microbiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Polymorphism</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: microbiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Restriction Fragment Length</style></keyword><keyword><style  face="normal" font="default" size="100%">Seasonality</style></keyword><keyword><style  face="normal" font="default" size="100%">Seasons</style></keyword><keyword><style  face="normal" font="default" size="100%">Trees</style></keyword><keyword><style  face="normal" font="default" size="100%">Trees: microbiology</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year></dates><publisher><style face="normal" font="default" size="100%">Blackwell Publishing Ltd</style></publisher><volume><style face="normal" font="default" size="100%">14</style></volume><pages><style face="normal" font="default" size="100%">565-575</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">We explored the changes in richness, diversity and evenness of epiphytic (on the leaf surface) and endophytic (within leaf tissues) bacteria and fungi in the foliar phyllosphere of Quercus ilex, the dominant tree species of Mediterranean forests. Bacteria and fungi were assessed during ontogenic development of the leaves, from the wet spring to the dry summer season in control plots and in plots subjected to drought conditions mimicking those projected for future decades. Our aim was to monitor succession in microbiota during the colonisation of plant leaves and its response to climate change. Ontogeny and seasonality exerted a strong influence on richness and diversity of the microbial phyllosphere community, which decreased in summer in the whole leaf and increased in summer in the epiphytic phyllosphere. Drought precluded the decrease in whole leaf phyllosphere diversity and increased the rise in the epiphytic phyllosphere. Both whole leaf bacterial and fungal richness decreased with the decrease in physiological activity and productivity of the summer season in control trees. As expected, the richness of epiphytic bacteria and fungi increased in summer after increasing time of colonisation. Under summer dry conditions, there was a positive relationship between TRF (terminal restriction fragments) richness and drought, both for whole leaf and epiphytic phyllosphere, and especially for fungal communities. These results demonstrate that changes in climate are likely to significantly alter microbial abundance and composition of the phyllosphere. Given the diverse functions and large number of phyllospheric microbes, the potential functional implications of such community shifts warrant exploration.</style></abstract><accession-num><style face="normal" font="default" size="100%">22289059</style></accession-num></record></records></xml>